High content screening microscope with life cell imaging unit

带生命细胞成像装置的高内涵筛选显微镜

• 批准号: 440386083
• 金额: --
• 依托单位: Heinrich-Heine-Universität Düsseldorf
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/440386083?language=en
• 关键词: content; screening; microscope; life; cell

For many years, the Willbold group has been investigating a wide variety of questions in in vitro cell cultures. These include studies on signalling pathways in autophagy or the elucidation of the mode of action of drug candidates developed by Willbold group for the therapy of neurodegenerative diseases. With the currently available instrumentation, these projects are limited in terms of experimental design. Living cells cannot be assayed for longer than a couple of minutes, since all microscopes in the institute lack a life cell imaging unit, which provides a constant temperature of 37 °C at 5 % CO2. Without this unit, the cells are therefore under stress at the time of analysis, which negates the significance of the results obtained. Time-dependent analysis, e. g. of vesicle transport processes, absolutely requires a microscope with a life cell imaging unit. A confocal system is mandatory as well, since it allows Z-stacking and minimizes background fluorescence from out-of-focus light. Moreover, all applications benefit from a high degree of automation, high throughput and high speed. Especially, the diagnostic sFIDA (surface-based fluorescence intensity distribution analysis) technology requires an automated microscope for high throughput screening of large sample numbers. The existing devices do not meet these requirements in any way. The applied high-throughput microscope with life cell imaging unit is therefore essential for the projects of the Willbold group.

多年来，威尔博德小组一直在研究体外细胞培养中的各种问题。其中包括对自噬信号通路的研究，或阐明 Willbold 小组开发的用于治疗神经退行性疾病的候选药物的作用方式。使用目前可用的仪器，这些项目在实验设计方面受到限制。活细胞的分析时间不能超过几分钟，因为该研究所的所有显微镜都缺乏生命细胞成像装置，该装置无法在 5% CO2 的条件下提供 37°C 的恒温。如果没有这个单元，细胞在分析时就会受到压力，从而否定了所获得结果的意义。时间相关分析，e。 g。囊泡运输过程的研究绝对需要带有生命细胞成像装置的显微镜。共焦系统也是必需的，因为它允许 Z 轴堆叠并最大限度地减少离焦光的背景荧光。此外，所有应用都受益于高度自动化、高吞吐量和高速度。特别是，诊断 sFIDA（基于表面的荧光强度分布分析）技术需要自动显微镜来对大量样本进行高通量筛选。现有设备无论如何都不能满足这些要求。因此，具有生命细胞成像装置的高通量显微镜对于 Willbold 小组的项目至关重要。