Transcriptional activation of the Drosophila melanogaster glucose-6- phosphate dehydrogenase gene by insertion of defective P elements
通过插入有缺陷的 P 元件来转录激活果蝇葡萄糖-6-磷酸脱氢酶基因
基本信息
- 批准号:04640590
- 负责人:
- 金额:$ 1.28万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1992
- 资助国家:日本
- 起止时间:1992 至 1993
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Tandem insertions of defective P elements (1.15 kb KP and 0.6 kb core P) accelerate the transcription rate of the glucose-6-phosphate dehydrogenase (G6PD) gene in Drosophila melanogaster. In this resarch, we have analyzed the activation mechanism of G6PD promoter by in vitro transcription and gel retardation assays. Results showed that on cis-acting region in the core P and two such regions in the KP are associated with the activation of G6PD promoter, and that putative transcriptional regulatory protein(s) which specifically bind to each of the cis-acting regions are present in nuclear extracts of Canton S embryos. On the other hand, the P elements do not activate the normal actin 5C promoter, but activate the promoter when the 20 bp sequence around the G6PD transcription start site is placed in front of the promoter. It seems that the GC-rich region in the 20 bp sequence is required for the activation.
串联插入有缺陷的P元件(1.15 kb KP和0.6 kb核心P)可加速果蝇葡萄糖-6-磷酸脱氢酶(G6 PD)基因的转录速率。本研究通过体外转录实验和凝胶阻滞实验分析了G6 PD启动子的激活机制。结果表明,核心P区的一个顺式作用区和KP区的两个顺式作用区与G6 PD启动子的激活有关,并且在Canton S胚胎的核提取物中存在与每个顺式作用区特异性结合的推定的转录调节蛋白。另一方面,P元件不激活正常肌动蛋白5C启动子,但当G6 PD转录起始位点周围的20 bp序列置于启动子之前时激活启动子。似乎20 bp序列中富含GC的区域是激活所需的。
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Ito Hiroki: "Transcriptional activation of the Drosophila melunogaster glucose-6-phosphate dehydrogenase gene by insertion of defective P elements" Molecular and General Genetics. 241. 637-646 (1993)
Ito Hiroki:“通过插入有缺陷的 P 元件来转录激活果蝇葡萄糖-6-磷酸脱氢酶基因”《分子与普通遗传学》。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Ito Hiroki: "Transcriptional activation of the Drosophila melanogaster glucose-6-phosphate dehydrogenase gene by insertion of defective Pelements" Molecular and General Genetics. 241. 637-646 (1993)
Ito Hiroki:“通过插入有缺陷的 P 元素来转录激活果蝇葡萄糖-6-磷酸脱氢酶基因”《分子与普通遗传学》。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Ito Hiroki: "Transcriptional activation of the Drosophila melanogaster blucose-6-phosphate dehydrogenase gene by insertion of defective P elements." Molecular and General Genetics. Vol.241. 637-646 (1993)
Ito Hiroki:“通过插入有缺陷的 P 元件来转录激活果蝇 6-磷酸脱氢酶基因。”
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- 影响因子:0
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HORI Hiroshi其他文献
HORI Hiroshi的其他文献
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{{ truncateString('HORI Hiroshi', 18)}}的其他基金
Cryotrapped reaction intermediates in cytochrome P450 and kineticsanalyses by newly developed rapid-freeze-quench EPR spectroscopy
细胞色素 P450 中的冷冻捕获反应中间体以及通过新开发的快速冷冻淬灭 EPR 光谱进行动力学分析
- 批准号:
19550165 - 财政年份:2007
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Comparative genomics of chromosome rearrangement in vertebrates based on medaka genome information
基于青鳉基因组信息的脊椎动物染色体重排的比较基因组学
- 批准号:
17018016 - 财政年份:2005
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Micro-environment of Fe-Cu binuclear center in Cytoobrome Oxidase Studied by Electron Paramagnetic Resonance Spectroscopy
电子顺磁共振波谱研究细胞溴氧化酶Fe-Cu双核中心的微环境
- 批准号:
13680741 - 财政年份:2001
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$ 1.28万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Transpososition of Tol-2 element by means of transposae mRNA injection into medakafish eggs
通过将转座 mRNA 注射到青鳉鱼卵中来转座 Tol-2 元件
- 批准号:
10640601 - 财政年份:1998
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Construction of gene tagging system by means of the medaka fish transposable element Tol-2
青鳉鱼转座元件Tol-2基因标记系统的构建
- 批准号:
09554053 - 财政年份:1997
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Crystallization and structural analysis of binary complex of P450 electron donor and its electron transfer mechanism
P450电子给体二元配合物的结晶、结构分析及其电子转移机制
- 批准号:
09680651 - 财政年份:1997
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The Tyrosinase Gene from Medakafish : Transgenic expression Rescues Albino Mutation
来自青鳉鱼的酪氨酸酶基因:转基因表达拯救白化突变
- 批准号:
08640786 - 财政年份:1996
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
TRANSGENIC ANALYSIS OF MAJOR HISTOCOMPATIBILITY COMPLEX IN MEDAKA FISH : ITS MOLECULAR EVOLUTION AND GENETIC POLYMORPHISM
青鳉鱼主要组织相容性复合体的转基因分析:分子进化和遗传多态性
- 批准号:
05404002 - 财政年份:1993
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for General Scientific Research (A)
Study of the Active Site Structure of Myeloperoxidase
髓过氧化物酶活性位点结构的研究
- 批准号:
04680270 - 财政年份:1992
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Genetics of Major Histocompotibility Complex
主要组织相容性复合体的遗传学
- 批准号:
03640539 - 财政年份:1991
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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