Transcriptional activation of the Drosophila melanogaster glucose-6- phosphate dehydrogenase gene by insertion of defective P elements

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Tandem insertions of defective P elements (1.15 kb KP and 0.6 kb core P) accelerate the transcription rate of the glucose-6-phosphate dehydrogenase (G6PD) gene in Drosophila melanogaster. In this resarch, we have analyzed the activation mechanism of G6PD promoter by in vitro transcription and gel retardation assays. Results showed that on cis-acting region in the core P and two such regions in the KP are associated with the activation of G6PD promoter, and that putative transcriptional regulatory protein(s) which specifically bind to each of the cis-acting regions are present in nuclear extracts of Canton S embryos. On the other hand, the P elements do not activate the normal actin 5C promoter, but activate the promoter when the 20 bp sequence around the G6PD transcription start site is placed in front of the promoter. It seems that the GC-rich region in the 20 bp sequence is required for the activation.