Cryotrapped reaction intermediates in cytochrome P450 and kineticsanalyses by newly developed rapid-freeze-quench EPR spectroscopy
Cryotrapped reaction intermediates in cytochrome P450 and kineticsanalyses by newly developed rapid-freeze-quench EPR spectroscopy
批准号:
19550165
负责人:
HORI Hiroshi
金额:
$2.41万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2007
资助国家:
日本
项目状态:
已结题
起止时间:
2007 至 2008
中文摘要
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英文摘要
サブミリ秒領域で酵素反応の進行を停止させ、捕捉した反応中間体を電子スピン共鳴法で検出・同定するため、再現性の良い急速混合凍結システムを開発した(不感時間 0.2 ms)。酸化型シトクロムP450camと過酢酸の反応でポルフィリンπカチオンラジカル中間体分子種の捕捉・同定に成功した。還元型P450cam・電子供与体複合体と酸素分子との急速混合凍結実験で還元型ヘム鉄に結合している酸素分子が1電子還元されたペルオキシド型反応中間体分子種の捕捉に初めて成功した
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
金属タンパク質の電子常磁性共鳴(EPR)測定をしようとする人のために
对于那些尝试使用电子顺磁共振 (EPR) 测量金属蛋白的人
DOI:
--
发表时间:
2008
期刊:
蛋白質科学会アーカイブ(Web公開誌) 1#037
影响因子:
--
作者:
[堀洋]
通讯作者:
堀洋
急速混合凍結EPR法によるP450cam反応サイクルにおける新しい反応中間体の観測
利用快速混合冷冻EPR法观察P450cam反应循环中新的反应中间体
DOI:
--
发表时间:
2007
期刊:
影响因子:
--
作者:
[D. Sugiyama, Y. Hirakawa, S. Osada, I. Fujita, Y. Hamasaki, and H. Kodama, 堀洋]
通讯作者:
堀洋
Comparative genomics of chromosome rearrangement in vertebrates based on medaka genome information
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批准号:17018016
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项目类别:Grant-in-Aid for Scientific Research on Priority Areas
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资助金额:$59.84万
-
财政年份:2005
-
负责人:HORI Hiroshi
-
依托单位:
Micro-environment of Fe-Cu binuclear center in Cytoobrome Oxidase Studied by Electron Paramagnetic Resonance Spectroscopy
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批准号:13680741
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.11万
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财政年份:2001
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负责人:HORI Hiroshi
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依托单位:
Transpososition of Tol-2 element by means of transposae mRNA injection into medakafish eggs
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批准号:10640601
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.54万
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财政年份:1998
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负责人:HORI Hiroshi
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依托单位:
Construction of gene tagging system by means of the medaka fish transposable element Tol-2
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批准号:09554053
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$5.44万
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财政年份:1997
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负责人:HORI Hiroshi
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依托单位:
Crystallization and structural analysis of binary complex of P450 electron donor and its electron transfer mechanism
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批准号:09680651
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.66万
-
财政年份:1997
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负责人:HORI Hiroshi
-
依托单位:
The Tyrosinase Gene from Medakafish : Transgenic expression Rescues Albino Mutation
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批准号:08640786
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.34万
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财政年份:1996
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负责人:HORI Hiroshi
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依托单位:
TRANSGENIC ANALYSIS OF MAJOR HISTOCOMPATIBILITY COMPLEX IN MEDAKA FISH : ITS MOLECULAR EVOLUTION AND GENETIC POLYMORPHISM
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批准号:05404002
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项目类别:Grant-in-Aid for General Scientific Research (A)
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资助金额:$10.18万
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财政年份:1993
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负责人:HORI Hiroshi
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依托单位:
Transcriptional activation of the Drosophila melanogaster glucose-6- phosphate dehydrogenase gene by insertion of defective P elements
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批准号:04640590
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.28万
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财政年份:1992
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负责人:HORI Hiroshi
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依托单位:
Study of the Active Site Structure of Myeloperoxidase
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批准号:04680270
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.22万
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财政年份:1992
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负责人:HORI Hiroshi
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依托单位:
Genetics of Major Histocompotibility Complex
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批准号:03640539
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.22万
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财政年份:1991
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负责人:HORI Hiroshi
-
依托单位:
A novel fluorescent method for detection of subpicogram quantities of nucleic acids
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批准号:02559002
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项目类别:Grant-in-Aid for Developmental Scientific Research (B)
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资助金额:$4.29万
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财政年份:1990
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负责人:HORI Hiroshi
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依托单位:
Positive regulation of the Drosophila melanogaster G6PD gene by a transposable element: its structure and function.
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批准号:63540499
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.22万
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财政年份:1988
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负责人:HORI Hiroshi
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依托单位:
Molecular evolution of algae and emergence of chloroplasts as deduced from 5S ribosomal RNA sequences.
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批准号:60540406
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$0.96万
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财政年份:1985
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负责人:HORI Hiroshi
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依托单位:
海外基金