Construction of gene tagging system by means of the medaka fish transposable element Tol-2

青鳉鱼转座元件Tol-2基因标记系统的构建

• 批准号: 09554053
• 负责人: HORI Hiroshi
• 金额: $ 5.44万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09554053/
• 关键词: medaka; Oryzias latipes; transposable element; Tol-2; tagging

Several transposable elements of the terminal-inverted-repeat class have been described in vertebrate genomes. Some of them are considered to be active because of the presence in genic regions or polymorphic situations of inserted elements. However, detection of de novo excision or insertion of the elements has not been successfully done. We have identified a novel terminal-inverted-repeat class transposable element in the medaka fish Oryzias latipes. The element was designated Tol-2. Excision of Tol-2 during embryogenesis was detected by a PCR analysis of a genomic region containing a Tol-2 copy. Tol-2 is 4681 bp in length. Tol-2 contains 4 ORFs which have amino acid sequence similarity with Ac of maize, hobo of Drosophila and Tam3 of snapdragon, which are all active transposable elements. Tol-2 is thus a unique material for establishing a gene tagging system in fish.

在脊椎动物基因组中已经描述了几个末端反向重复类的转座因子。其中一些被认为是活跃的，因为在基因区域或多态性的插入元件的存在。然而，从头切除或插入的元件的检测尚未成功完成。我们已经确定了一个新的终端反向重复类转座因子青鳉鱼。该元素被命名为Tol-2。通过PCR分析含有Tol-2拷贝的基因组区域来检测胚胎发生期间Tol-2的切除。Tol-2全长4681 bp。Tol-2含有4个ORF，与玉米的Ac、果蝇的流浪汉和金鱼草的Tam 3氨基酸序列相似，均为活性转座因子。因此，Tol-2是建立鱼类基因标签系统的独特材料。

项目成果

Hori H: "Excision of the Tol-2 transposable element of the medakafish,Oxzias latipes,in zebrafish,Danio rerio." Gene. 225(1). 17-22 (1998)

Hori H：“在斑马鱼、斑马鱼中切除青鳉鱼 Oxzias latipes 的 Tol-2 转座元件。”

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Ozato K: "An embryological study of centralization of dorsal structures in the tail of medaka (Oryzios latipes) Da Mutants" Develop.Growth Differ. 39. 531-538 (1997)

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Koga A: "Homogeneity in the structure of the medakafish transposable element" Genetical Research. 73. 7-14 (1999)

Koga A：“青鳉鱼转座元件结构的同质性”遗传学研究。

Makita R,Mizuno T,Koshida S,Kuroiwa A,Takeda H.: "Zebrafish wnt11 : pattern and regulation of the expression by the yolk cell and No tail activity." Mech.Dev.71(1-2). 165-76 (1998)

Makita R、Mizuno T、Koshida S、Kuroiwa A、Takeda H.：“斑马鱼 wnt11：卵黄细胞表达的模式和调节以及无尾活性。”