Genetics of Major Histocompotibility Complex

主要组织相容性复合体的遗传学

• 批准号: 03640539
• 负责人: HORI Hiroshi
• 金额: $ 1.22万
• 依托单位: Dept. Biol., Facl. Sci., Nagoya Univ. (1992)Hiroshima University (1991)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03640539/
• 关键词: MHC; Genetic Polymorphism; HLA; SSCP; Matching; Transplantation; 主要組織適合抗原系; DQB1; DRB1; デ-タベ-ス

The MHC(Major Histocompatibility Complex, HLA in human) antigens are highly polymorphic glycoproteins expressed on the cell surface of all tissues (class I MHC) and antigen-presenting cells (class II MHC). These antigens play a key role in immune recognition of self and non-self antigens by T cells. Allologous MHC antigens are involved in graft rejection in tissue transplantation.While MHC can be serologically defined in common clinical cases, recent advances in molecular biological techniques, especially the development of polymerase chain reaction (PCR), have facilitated more accurate analysis of the polymorphism in MHC at the DNA level. In this project, we have developed a rapid typing method based on the detection of single-stand conformation polymorphism, and formation of heteroduplexes in PCR products.

MHC（主要组织相容性复合物，人类中的HLA）抗原是在所有组织（I类MHC）和抗原呈递细胞（II类MHC）的细胞表面上表达的高度多态性糖蛋白。这些抗原在T细胞对自身和非自身抗原的免疫识别中起关键作用。组织移植排斥反应的发生与MHC抗原的等位基因有关，临床上常见的MHC抗原可通过血清学方法确定，而近年来分子生物学技术的发展，特别是聚合酶链反应（PCR）技术的发展，使得在DNA水平上对MHC抗原多态性的分析更为准确。本研究以PCR产物中单链构象多态性和异源双链形成为基础，建立了一种快速分型方法。

项目成果

Hori,H.,Satow,Y.,Inoue,I. Chihara,M.: "Archaebacteria us Metabocteria : phylogenetic tree of Organisms indicated by comparison of 5S rRNA sequences" Evolution of Life (Osawa,S.,Honjo,T.eds.) Springer,Tokyo/Berlin. 325-336 (1991)

堀H.、佐藤Y.、井上I.

Hori,H.: "Evolutionary outline of living organisms as deduced from 5S rRNA sequences" Bio diuersity and Global Change (S.lbrig,OT.et al.eds.),IUBS,Paris. 95-104 (1992)

Hori,H.：“从 5S rRNA 序列推导出的生物体的进化轮廓”生物多样性和全球变化（S.lbrig，OT.et al.eds.），IUBS，巴黎。

Kimura,A.,Fukuda,Y.,Hori,H.,Hoshino,S.,Sasazuki,T.,Dohi,Y.: "Polymerase chain reaction -single-strand conformation polymorphism analysis of HLA-DP genes and its application in transplantation." Transplantation Proceedings. 25. 199-202 (1993)

Kimura,A.,Fukuda,Y.,Hori,H.,Hoshino,S.,Sasazuki,T.,Dohi,Y.：“聚合酶链式反应-HLA-DP基因的单链构象多态性分析及其应用

Kimura,A.,Fukuda,Y.,Hori.,H,Hoshino,S.,Sasazuki,T: "Polymerase chain reaction-single-strand conformation polymorphism analysis of HLA-DPgenes and its application in transplantotion" Transplantation proceedings. 25. 199-202 (1993)

Kimura,A.,Fukuda,Y.,Hori.,H,Hoshino,S.,Sasazuki,T：“HLA-DP基因的聚合酶链反应-单链构象多态性分析及其在移植中的应用”移植程序。

Hori,H.,Satow,Y.: "Dead-end evolution of the Cnidaria as deduced from 5S ribosomal RNA sequences" Hydrobiologia. 216. 505-508 (1991)

Hori,H.,Satow,Y.：“从 5S 核糖体 RNA 序列推论出的刺胞动物的死胡同进化”Hydrobiologia。