Effects of retinoic acid on limb bud pattern formation and limb blastema pattern formation

视黄酸对肢芽模式形成和肢芽基模式形成的影响

• 批准号: 04640650
• 负责人: IDE Hiroyuki
• 金额: $ 1.15万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04640650/
• 关键词: Retinoic Acid; Limb bud; Regeneration; Pettern formation; 細胞親和性

To examine common mechanisms of Retinoic acid (RA)-induced pattern formation in the developing limb bud and regenerating limb blastema, chick limb bud at satge 20 was treated with RA for 10 hours by implanting RA-bead and then the mesenchymal tissue near the bead was transplanted to the anterior margin of otherlimb bud at stage 18-19. The RA-treated and transplanted tissues formed all the cartilage elements from humerus to degits, although the control transplants formed only digits according to their fate. This "proximalization" occurred independently of posteriorization, that is, induction of ZPA.The expression pattern of HoxA, which was known to change corresponding to the proximodistal positional value in normal limb development, changed to show the proximalization in RA-treated transplants. The cell affinity also changes according to the positional values. The dissociated cells of RA-treated mesenchyme showed the proximalization to segregate from those of untreated tissues in monolayr culture. These results suggest that the cells of RA-treated mesenchyme of developing limb changes their positional value to show proximalization other than posteriorization as shown previously in regeneration blastema of salamander limb.

为探讨维甲酸（RA）诱导发育中的肢芽和再生肢芽模式形成的共同机制，将RA珠植入20龄鸡肢芽10 h，然后将RA珠附近的间充质组织移植到18 ~ 19龄鸡肢芽前缘。RA处理和移植的组织形成了从肱骨到手指的所有软骨成分，尽管对照移植物根据其命运仅形成手指。这种“近端化”的发生独立于后向化，即ZPA的诱导。HoxA的表达模式，这是已知的改变相应于proximodistal位置值在正常肢体发育，改变显示在RA处理的移植物的近端化。细胞亲和性也根据位置值而改变。在单层培养中，RA处理的间充质细胞与未处理的间充质细胞表现出接近分离。这些结果表明，RA处理的发育肢体间充质的细胞改变其位置值，以显示proximalization而不是posteriorization如先前所示的蝾螈肢体再生芽基。

项目成果

Ide, H., Wade, N.and Uchiyama, K.: "Sorting out of cells from different parts and stages of the chick limb bud" Developmental Biology. 162. 71-76 (1994)

Ide, H.、Wade, N. 和 Uchiyama, K.：“从鸡肢芽的不同部位和阶段中分选细胞”发育生物学。

Ide,H.,Wada,N., Uchiyama,K.and Kameyame,T.: "Position-specific chondrogenesis of chick limb bud cells in culture. 章名 Limb Regeneration and Development 本名" Wiley, (1993)

Ide, H.、Wada, N.、Uchiyama, K. 和 Kameyame, T.：“培养中鸡肢芽细胞的位置特异性软骨形成。章节名称肢体再生和发育真名”Wiley，(1993)

Watanabe,A.: "Basic FGF maintains some characteristics of the progress zone of chick limb bud in cell culture" Developmental Biology. 159. 223-231 (1993)

Watanabe,A.：“基本 FGF 保持了细胞培养中小鸡肢芽发育区的一些特征”《发育生物学》。

Tamura, K.and Ide, H.: "The function of retinoic acid in chick limb pattern formation : posterorization and proximalization by RA" Limb Development and Regeneration. B. 827-836 (1993)

Tamura, K. 和 Ide, H.：“视黄酸在小鸡肢体模式形成中的功能：RA 的后序化和近端化”肢体发育和再生。

Tamura,K., Aoki,Y. and Ide,H.: "Induction of polarizing activity by retinoic acid occurs independently of duplicate formation in developing chick limb buds." Developmental Biology. (1993)

田村，K.，青木，Y.