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Molecular mechanisms of cell adhesion corresponding to positional values in limb bud

肢芽位置值对应的细胞粘附的分子机制

基本信息

批准号：
06404084
负责人：
IDE Hiroyuki
金额：
$ 13.63万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06404084/
关键词：
cell adhesion molecule cell sorting cadherin limb bud pattern formation 位置価

项目摘要

In vertebrate limb development, limb mesenchyme cells in the progress zone form cartilage pattern depending on the positional values allotted to them. The limb bud cells are though to use cell surface molecules to recognize the positional values of their surrounding cells.We have found the difference in cell surface molecules from the cell sorting experiment using chick limb mesenchyme with different positional values. The sorting out in monolayr culture occurred when the mesenchymal cells at different developmental stages or from different positions were mixed. The sorting out occurred independently of chondrogenic potency and cartilage nodules were formed in the center of each sorted area. The mesenchymal cells with different positional values were sorted even on agar where cells could not attach and form aggregates, suggesting the sorting out occurs depending on the difference on not cell-substratum but cell-cell affinity.To identify cell adhesion molecules which control the positional value-related cell adhesion, we first cloned cDNA of cadherin superfamily genes expressing in chick limb bud with RT-PCR.Protocadherin 2, a homologue of human protocadherin Pcdh2, was identified. The expression pattern of protocadherin 2 suggests its close relationship to cell sorting.We have raisd a monoclonal antibody to the cell surface molecular of chick limb bud mesenchyme, which inhibited the sorting out between the progress zone cells at different developmental stages.

在脊椎动物肢体发育中，发育区中的肢体间充质细胞根据分配给它们的位置值形成软骨图案。肢芽细胞是通过细胞表面分子来识别周围细胞的位置值的，我们通过对不同位置值的鸡肢间充质细胞进行分选实验，发现了细胞表面分子的差异。在单层培养中，不同发育阶段或不同部位的间充质细胞混合时，会出现分选现象。分选发生独立的软骨形成的潜力和软骨结节形成在每个分选区域的中心。为了鉴定控制与位置值相关的细胞粘附的细胞粘附分子，我们首先用RT-PCR方法克隆了鸡肢芽中表达的钙粘素超家族基因的cDNA，原钙粘素2（Protocadherin 2，Protocadherin 2），鉴定了人原钙粘蛋白Pcdh 2的同源物。原钙粘素2的表达模式表明其与细胞分选密切相关，我们制备了鸡肢芽间充质细胞表面分子的单克隆抗体，该抗体可抑制不同发育阶段发育区细胞之间的分选。