Studies on the Molecular Mechanisms of Cartilage Pattern Formation in Chick Limb Bud

鸡肢芽软骨模式形成的分子机制研究

• 批准号: 01480023
• 负责人: IDE Hiroyuki
• 金额: $ 3.14万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480023/
• 关键词: cartilage pattern; retinoic acid; molecular mechanism; 肢芽; パタ-ン形成; 軟骨分化

To elucidate the molecular mechanisms of cartilage pattern formation of chick limb bud, retinoic acid (RA), which was known to induce limb duplication, was locally applied to the various regions of the limb bud or added to the limb mesodermal cells in culture. RA applied to the antero-distal region of the limb bud induced ZPA activity and stimulated the expression of RARbeta mRNA. The induction of ZPA activity was independently of duplicate formation and depended on the presence of AER at the distal top of the limb bud.RA induced the formation of 18kD protein in limb bud cell culture. This induction occurred only in the cells of anterior and distal region. Induction of ZPA activity in cell culture also occurred only in these cells, suggesting the role of 18kD protein in ZPA induction.RA promotes the proliferation and chondrogenesis in cultured distal mesodermal cells of limb bud. This promotion also occurred in the cells of antero-distal region.These results suggest a common mechanism in the induction of ZPA activity in vivo and in vitro and the promotion of cell proliferation and chondrogenesis by RA. .

为了阐明鸡肢芽软骨形态形成的分子机制，我们将维甲酸（retinoic acid， RA）局部应用于鸡肢芽的不同区域或添加到培养的鸡肢中胚层细胞中。RA作用于肢芽前远端区，诱导ZPA活性，刺激RARbeta mRNA的表达。ZPA活性的诱导不依赖于重复的形成，而依赖于肢芽远端顶端AER的存在。RA诱导肢体芽细胞18kD蛋白的形成。这种诱导只发生在前区和远区细胞。在细胞培养中，ZPA活性的诱导也只发生在这些细胞中，提示18kD蛋白在ZPA诱导中的作用。RA能促进培养的肢芽远端中胚层细胞的增殖和软骨形成。这种促进也发生在前远端区细胞。这些结果提示RA在体内和体外诱导ZPA活性，促进细胞增殖和软骨形成的共同机制。

项目成果

Ide H., Ohkubo Y., Noji S. Tanicuchi S.: "Retinoic Acid and Limb Pattern Formation in Cell Culture." Developmental Patterning of the Vertebrate Limb. eds. Hinchliffe et al.105-113 (1991)

Ide H.、Ohkubo Y.、Noji S. Tanicuchi S.：“细胞培养中的视黄酸和肢体模式形成”。

Noji,S.,Nohno,T.,Koyama,E.,Muto,K.,Ohyama,K.,Aoki,Y.,Tamura,K.,Ohsugi,K.,Ide,H.,Taniguchi,S.,and Saito,T.: "Retinoic acid induces polarizing activity but is unlikelyto be a morphogen in the chick limb bud." Nature. 350. 83-86 (1991)

野司，S.，野野，T.，小山，E.，武藤，K.，大山，K.，青木，Y.，田村，K.，大杉，K.，井出，H.，谷口，S.，

Ide,H.: "Growth and differentiation of limb bud cells in vitro:Implications for limb pattern formation." Development,Growth and differentiation. 32. 1-8 (1990)

Ide,H.：“肢芽细胞的体外生长和分化：对肢体模式形成的影响。”

Noji S., Nohno T., Koyama E., Muto K., Ohyama K., Aoki Y., Tamura K., Ohsugi K., Ide H., Taniguchi S., Saito T.: "Retinoic Acid Induces Polarizing Activity But is Unlikely To Be a Morphogen in the Chick Limb Bud." Nature. 350. 83-86 (1991)

Noji S.、Nohno T.、Koyama E.、Muto K.、Ohyama K.、Aoki Y.、Tamura K.、Ohsugi K.、Ide H.、Taniguchi S.、Saito T.：“视黄酸诱导偏振活性

Yokouchi Y.: "Control of the expression of AVー1 protein,a positionーspecific antigen,by ZPA factor in the chick limb bud." Develop.Growth & differ.33. 173-180 (1991)

Yokouchi Y.：“鸡肢芽中 ZPA 因子控制 AV-1 蛋白（一种位置特异性抗原）的表达。” Develop.Growth & Difference.33 (1991)。