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Studies on Pattern Formation in Chick Limb Bud.

鸡肢芽图案形成的研究。

基本信息

批准号：
61540510
负责人：
IDE Hiroyuki
金额：
$ 1.09万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

1. Growth factor of ZPA.(1) By the use of organ culture of chick limb bud fragments, the presence of growth factor(s) in the posterior region including ZPA and the responsiveness of the anterior region to the factor have been demonstrated.(2) The limb bud was divided into four regions along the anteroposterior axis, and dissociated cells of each region were cocultured with the fragments of the posterior region. The proliferation of the cells from the anterior region was promoted by the coculture, and a gradient of responsiveness to the growth promoting factor was found. No promotion was observed when cultured with anterior fragments.(3) The presence of the gradient of responsiveness was examined on some known growth factors. Fibroblast growth factor(FGF) showed similar pattern of growth promotion, suggesting that ZPA produces and releases FGF-like growth factor.(4) Growth promotion effect of retinoic acid, a canidate of morphogen in limb bud morphogenesis, was examined. In retina-conditoned medium, the retinoic acid at 10-100ng/ml promoted the proliferation and cartilage nodule formation of distal mesodermal cells.2. Limb bud specific protein.Using the method of two dimensional electrophoresis and silver staining. a limb specific protein, L-1, has been found. The study on the distribution of the protein in various limb bud region is now in progress.3. Limb bud region specific antigen, AV-1.The position specific antigen, AV-1, was found to be a glycoprotein with a molecular weight of 135kD.

1. ZPA生长因子。(1)利用鸡肢芽片段的器官培养，证实了生长因子(s)在鸡肢后区（包括ZPA）的存在以及鸡肢前区对该因子的反应性。(2)将肢芽沿前后轴分成4个区域，每个区域的游离细胞与后区域的碎片共培养。共培养能促进前区细胞的增殖，对生长促进因子的反应呈梯度。与前片培养时未观察到促进作用。(3)对一些已知的生长因子进行了反应性梯度的检验。成纤维细胞生长因子（Fibroblast growth factor， FGF）表现出类似的促生长模式，提示ZPA产生并释放FGF样生长因子。(4)考察了维甲酸在肢体芽形态发生中的促生长作用。在视网膜条件培养基中，10-100ng/ml维甲酸促进远端中胚层细胞增殖和软骨结节形成。肢芽特异性蛋白。采用二维电泳和银染色的方法。发现了肢体特异性蛋白L-1。目前对该蛋白在不同肢芽区分布的研究正在进行中。肢芽区特异性抗原AV-1。位置特异性抗原AV-1是一个分子量为135kD的糖蛋白。