Establishment of Limb Regeneration System in Mouse

为建立高等脊椎动物肢体再生系统，对小鼠肢体再生进行了研究，将新生小鼠的前肢进行不同程度的截肢，两天后在截肢部位局部应用生长因子蛋白，再生能力随截肢水平的不同而不同。前臂和手部水平截肢可引起残骨组织肥大。腕水平截肢可诱导残肢远端边缘软骨组织形成，截肢部位应用成纤维细胞生长因子-2、4蛋白促进骨组织肥大。局部应用成纤维细胞生长因子-8和成纤维细胞生长因子-10未观察到促进作用。BMP-2和BMP-4诱导截肢部位远端结构的形成，利用基因枪将成纤维细胞生长因子受体基因应用于截肢部位。同时注射绿色荧光蛋白表达，但软骨形态未见改变。这些结果表明，一些FGFs和BMPs被诱导了局部的增殖和分化，而已知的诱导非洲爪哇肢体再生的成纤维细胞生长因子-10没有刺激作用。

Induction of limb regeneration in mouse was studied to establish limb regeneration system in higher vertebrates.Forelimbs of new-born mouse were amputated at various level, and growth factor proteins were locally applied to the amputation sites after two days.The regeneration potency changed depending on amputation level. The limbs amputated at forearm and hand level induced the hypertrophy of stump bone tissues. The limbs amputated at wrist level induced the formation of cartilage tissues at the distal margin of stump tissues.FGF-2 and FGF-4 protein applied to the amputation sites promoted the hypertrophy of bone tissues. No promotion was observed by the local application of FGF-8 and FGF-10. BMP-2 and BMP-4 induced the formation of distal structures at the amputation site.Genes of FGF receptors were applied to the amputation sites by a gene-gun. Expression of GFP injected simultaneously was observed, but no changes in cartilage pattern occurred.These results suggest the local proliferation and differentiation was induced in some FGFs and BMPs, although no stimulation was observed by FGF-10, which is known to induce limb regeneration in Xenopus laevis.