Establishment of Limb Regeneration System in Mouse

为了建立高等脊椎动物的肢体再生系统，研究了诱导小鼠肢体再生的方法。在不同程度上切除新生小鼠前肢，2天后局部应用生长因子蛋白于截肢部位。再生能力随截肢程度的不同而变化。前臂和手部水平截肢引起残肢骨组织肥大。腕部水平截肢诱导残肢远端边缘软骨组织形成。FGF-2和FGF-4蛋白应用于截肢部位促进骨组织肥厚。局部应用FGF-8和FGF-10未观察到促进作用。BMP-2和BMP-4诱导截肢部位远端结构的形成。通过基因枪将FGF受体基因应用于截肢部位。同时注射GFP观察其表达，但软骨形态未发生变化。这些结果表明，尽管FGF-10未观察到刺激作用，但某些fgf和bmp可诱导局部增殖和分化，而已知FGF-10可诱导非洲爪蟾肢体再生。

Induction of limb regeneration in mouse was studied to establish limb regeneration system in higher vertebrates.Forelimbs of new-born mouse were amputated at various level, and growth factor proteins were locally applied to the amputation sites after two days.The regeneration potency changed depending on amputation level. The limbs amputated at forearm and hand level induced the hypertrophy of stump bone tissues. The limbs amputated at wrist level induced the formation of cartilage tissues at the distal margin of stump tissues.FGF-2 and FGF-4 protein applied to the amputation sites promoted the hypertrophy of bone tissues. No promotion was observed by the local application of FGF-8 and FGF-10. BMP-2 and BMP-4 induced the formation of distal structures at the amputation site.Genes of FGF receptors were applied to the amputation sites by a gene-gun. Expression of GFP injected simultaneously was observed, but no changes in cartilage pattern occurred.These results suggest the local proliferation and differentiation was induced in some FGFs and BMPs, although no stimulation was observed by FGF-10, which is known to induce limb regeneration in Xenopus laevis.