Photorhabdus Toxin Complex (PTC) as variable protein injection apparatus

光杆菌毒素复合物 (PTC) 作为可变蛋白质注射装置

• 批准号: 441232035
• 负责人: Professorin Dr. Gudula Schmidt
• 金额: --
• 依托单位: Abteilung I
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/441232035?language=en
• 关键词: Photorhabdus; Toxin; Complex; PTC; variable

The project aims to further characterize and to engineer the trimeric, stand alone toxin complex (Photorhabdus luminescens toxin complex: PTC), which allows injection of cargo proteins into mammalian cells. We already showed that loading and injection of different cargo molecules, including a very small luciferase for quantification was possible. Our recently published data show that packaging and injection of proteins with a size of up to 30 kDa is possible. The project aims to study, whether injection of mammalian proteins, for example regulators of small GTPases is possible. We plan to optimize the efficiency of the injection. Therefore, we plan to analyze the requirements of a cargo molecule to be actively released into mammalian cells. Our aim is to engineer the toxin complex as universal protein injection machinery for cell biological and possibly pharmacological use. To do so, it is necessary to identify the cellular structures allowing toxin binding (receptors), to mutate the corresponding amino acids of the toxin and to attach a peptide or small protein allowing more specific cell binding.

该项目旨在进一步表征和设计三聚体的独立毒素复合物（Photorhabdus luminescens toxin complex：PTC），该复合物允许将货物蛋白注射到哺乳动物细胞中。我们已经表明，装载和注射不同的货物分子，包括用于定量的非常小的荧光素酶是可能的。我们最近发表的数据表明，包装和注射的蛋白质的大小高达30 kDa是可能的。该项目旨在研究是否可以注射哺乳动物蛋白质，例如小GTP酶的调节剂。我们计划优化注射效率。因此，我们计划分析货物分子主动释放到哺乳动物细胞中的要求。我们的目标是将毒素复合物设计成用于细胞生物学和可能的药理学用途的通用蛋白质注射机器。为此，有必要鉴定允许毒素结合的细胞结构（受体），突变毒素的相应氨基酸，并连接允许更特异性细胞结合的肽或小蛋白。