Structural and functional basis of the complex of mono-ADP-ribosylating toxin and substrate protein

单ADP核糖基化毒素与底物蛋白复合物的结构和功能基础

• 批准号: 21570121
• 负责人: TSUGE Hideaki
• 金额: $ 3.08万
• 依托单位: Kyoto Sangyo University (2010-2011)Tokushima Bunri University (2009)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21570121/
• 关键词: C3; RhoA; Ia; アクチン; モノADPリボシル化; 結晶構造解析; 特異性; 複合体結晶構造解析; ADPリボシルトランスフェラーゼ; 細菌毒素; X線結晶構造解析; 複合体解析; C3毒素

To reveal the structural and functional mechanism of C. perfringens iota toxin(Ia) and actin, we successfully crystallized and solved the structure of the apo-Ia-actin and apo-Ia. We revealed the structural change upon the ADP-ribosylation by comparing the structures together with NADH-Ia and βTAD-Ia-actin complex.C. Botulinum C3 toxin ADP-ribosylates RhoA Asn41. For the structure analysis of C3-RhoA complex, RhoA was expressed in E. coli and the stable mutant of RhoAF25N was obtained using His-tag and GST-tag, independently. We are currently searching the crystallization condition for the complex.

为了揭示产气荚膜梭菌iota毒素(Ia)和肌动蛋白的结构和作用机制，我们成功地结晶并解决了apo-Ia-肌动蛋白和apo-Ia的结构。通过与NADH-Ia和βTAD-Ia-肌动蛋白复合体的结构比较，揭示了ADP-核糖化过程中的结构变化。C.肉毒C3毒素ADP-核糖化RhoA Asn41。在C3-RhoA复合体的结构分析中，我们在大肠杆菌中表达了RhoA，并分别利用His-Tag和GST-Tag获得了稳定的突变体RhoAF25N。我们目前正在寻找该络合物的结晶条件。

项目成果

Structural basis for the kexin-like serine protease from Aeromonas sobria as a sepsiscausing factor

温和气单胞菌中的科欣样丝氨酸蛋白酶作为败血症致病因子的结构基础

• 发表时间: 2009
• 期刊: J Biol Chem. 284-40
• 作者: Kobayashi H.;Tsuge H.;等
• 通讯作者: 等

The catalytic mechanism of dye‐decolorizing peroxidase DyP may require the swinging movement of an aspartic acid residue

染料脱色过氧化物酶 DyP 的催化机制可能需要天冬氨酸残基的摆动运动

• 发表时间: 2011
• 期刊: The FEBS Journal
• 作者: Toru Yoshida;H. Tsuge;H. Konno;T. Hisabori;Y. Sugano
• 通讯作者: Y. Sugano

Nucleolin as cell surface receptor for tumor necrosis factor-alpha inducing protein : a carcinogenic factor of Helicobacter pylori.

核仁素作为肿瘤坏死因子-α诱导蛋白的细胞表面受体：幽门螺杆菌的致癌因子。

• 发表时间: 2010
• 期刊: J Cancer Res Clin Oncol.
• 作者: Watanabe T;Tsuge H;Imagawa T;Kise D;Hirano K;Beppu M;Takahashi A;Yamaguchi K;Fujiki H;Suganuma M.
• 通讯作者: Suganuma M.

Non-native alpha-helix formation is not necessary for folding of lipocalin : comparison of burst-phase folding between tear lipocalin and beta-lactoglobulin

非天然 α 螺旋形成对于脂质运载蛋白的折叠来说不是必需的：泪液脂质运载蛋白和 β-乳球蛋白之间爆发相折叠的比较

• 发表时间: 2009
• 期刊: Proteins
• 影响因子: 2.9
• 作者: Tsukamoto S;Yamashita T;Yamada Y;Fujiwara K;Maki K;Kuwajima K;Matsumura Y;Kihara H;Tsuge H;Ikeguchi M
• 通讯作者: Ikeguchi M

ADPリボシル化毒素構造研究の最前線

ADP核糖基化毒素结构研究前沿

• 发表时间: 2011
• 作者: Shiozaki;K.;et al;津下英明
• 通讯作者: 津下英明