APROARCH TO RICE BACTERIAL BLIGHT RESISTANCE THROUGH MOLECULAR GENETICS

通过分子遗传学研究水稻白叶枯病抗性

• 批准号: 04660008
• 负责人: YOSHIMURA Atsushi
• 金额: $ 1.34万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660008/
• 关键词: Rice bacterial blight resistance; RFLP marker; RAPD marker; Near-isogenec ines; Chromosome walk; Combining resisntance genes; Single seed descent method; Quantitative resistnace; 染色体歩行

Genes for bacterial resistance of rice, Xa-1, Xa-3, Xa-4, xa-5, Xa-10 and Xa-11, were tagged with several RFLP and RAPD markers using near-isogenic lines and F2 populations. Xa-1 linked to several RFLP and RAPD markers with map distances of less than 5cM.Xa-3 and Xa-4 that are closely linked or allelic, linked to the same RFLP markers. Xa-10 showed the linkage with some RFLP and RAPD markers. The RAPD markeres linked to xa-5 and Xa-11 were also found. These RFLP and RAPD markers were useful for developing the combined lines of the bacterial blight resisitance genes.We developed the recombinant inbred (RI) lines as a permanent mappinng population for RFLP/RAPD markers and used them to analyze the quantitative resistance to bacterial blight. The RFLP/RAPD land mark map constructed using the RI population covered approximately 1090 cM containg 114 RFLP loci and 26 RAPD loci. Three putative loci affecting the quantitative resistance were identified in the preliminary study.

利用近等基因系和F2群体，对水稻细菌抗性基因Xa-1、Xa-3、Xa-4、xa-5、Xa-10和Xa-11进行了RFLP和RAPD标记。Xa-1与几个RFLP和RAPD标记连锁，图谱距离小于5cM。Xa-3和Xa-4是紧密相连的等位基因，与相同的RFLP标记相连。Xa-10与一些RFLP和RAPD标记有连锁关系。还发现了与xa-5和Xa-11相关的RAPD标记。这些RFLP和RAPD标记可用于开发抗白叶枯病基因组合系。我们开发了重组自交系（RI）作为RFLP/RAPD标记的永久定位群体，并利用它们对白叶枯病进行了定量抗性分析。利用RI种群构建的RFLP/RAPD地标图谱覆盖约1090 cM，包含114个RFLP位点和26个RAPD位点。初步研究确定了3个影响数量抗性的推定位点。

项目成果

Tsunematsu, H., H.Hasegawa, S.Yoshimura, A.Yoshimura and N.Iwata: "Construction of an RFLP/RAPD linkage map by using recombinant inbred lines." Rice Genetics Newsletter. 10. 89-90 (1993)

Tsunematsu, H.、H.Hasekawa、S.Yoshimura、A.Yoshimura 和 N.Iwata：“使用重组自交系构建 RFLP/RAPD 连锁图谱。”

Yoshimura.S,A.Yoshimura,R.Nelson,T.W.Mew and N.Iwata: "Tagging genes for bacterial blight resistance in rice by RFLP and RAPD markers." Agricultural Biotechnology,Proceeding of Asia-Pacific Conference on Agricultural Biotechnology,China Sci.Tech.Press.202

Yoshimura.S、A.Yoshimura、R.Nelson、T.W.Mew 和 N.Iwata：“通过 RFLP 和 RAPD 标记标记水稻白叶枯病抗性基因。”

Yoshimura, S., A.Yoshimura, A.Saito, N.Kishimoto, M.Kawase, M.Yano, M.Nakagahra, T.Ogawa and N.Iwata: "RFLP anasysis of introgressed chromosomal sgments in three near isogenic lines of rice for bacterial blight resistance genes, Xa-1, Xa-3 and Xa-4." Jpn.

Yoshimura, S., A.Yoshimura, A.Saito, N.Kishimoto, M.Kawase, M.Yano, M.Nakagahra, T.Okawa 和 N.Iwata：“RFLP 分析三个近等基因系中基因渗入的染色体片段

吉村 淳・岩田 伸夫: "育種学最近の進歩 第34集 第34回 日本育種学会シンボジウム報告28HD01:1993" 養賢堂, 6

Jun Yoshimura 和 Nobuo Iwata：“育种最新进展，第 34 卷，第 34 届日本育种学会研讨会报告 28HD01:1993” Yokendo，6

吉村 淳・斎藤 彰: "組織倍養18(4)「イネにおけるRFLP分析」" ニューサイエンス社, 6 (1992)

Jun Yoshimura 和 Akira Saito：“Tissue Expansion 18(4) ‘水稻中的 RFLP 分析’”New Science Publishing，6 (1992)