Rice domestication from the view of seed shattering genes

从落粒基因角度看水稻驯化

• 批准号: 13460004
• 负责人: YOSHIMURA Atsushi
• 金额: $ 8万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13460004/
• 关键词: Rice; Wild relative; Domestication; Seed shattering; Introgression lines; Gene isolation; Non-allelic interaction; Intragenic variation; 染色体断片導入系統

There are two cultivated rice species, Oryza sativa L. and O.glaberrima Steud. O.sativa is believed to be domesticated from a wild species, O.rufipogon Griff., whereas O.glaberrima has been differentiated probably from O.barthii Chev. et Roehr. Seed shattering habit is one of the most important traits for rice domestication. Therefore, the genes involved in seed shattering arc analyzed to elucidate the process of rice domestication. The project focused on a gene, Shattering3(Sh3), because the preliminary study revealed that Sh3 commonly existed in A-genome wild relatives of rice. The project consists of two parts : (1)Map-based cloning of Sh3 and (2)Clarification of the process of rice domestication.In the item (1),high-resolution mapping allowed us to narrow the genomic region of Sh3 to less than 5kb. There is one candidate ORF predicted in that region. However, transformation and sequencing analyses revealed that the ORF was not Sh3. We reviewed die results thoroughly and continuing … More the experiment.In the item (2),linkage mapping revealed that all of the wild species tested possessed Sh3. While O.glaberrima retains a functional Sh3 allele, it also carries a recessive sh9(iSh3) gene. The homozygotes for the recessive sh9 showed non-shattering despite of the background of functional Sh3. High-resolution mapping of Sh3 and sh9 showed that these two genes are tightly linked but on different loci. Moreover, O.glaberrima has the nucleotide change that potentially cause a premature stop codon in the ORF appeared in (1). This suggested that the ORF is a likely candidate of Sh9.The results of this project are summarized as follows :-:Loss-of-function of Sh3 was tightly related with rice domestication.-:Non-shattering O.glaberrima retains a functional Sh3 allele.-:sh9 gene tightly linked to Sh3 was identified. Loss-of-function of sh9 may be a possible cause of the domestication of O.glaberrima.We envision isolating Sh3 and sh9 to clarify the mechanism of rice domestication in the nucleotide level. Less

有两种栽培稻，Oryza sativa L。和光萼藓O.glaberrima Steud.栽培稻被认为是从野生种普通栽培稻驯化而来的，而O.glaberrima可能与O.barthii Chev.和罗尔。落种习性是水稻驯化的重要性状之一。因此，对水稻落粒相关基因的分析有助于阐明水稻的驯化过程。该项目的重点是一个基因，Shattering 3（Sh 3），因为初步研究表明，Sh 3普遍存在于水稻的A-基因组野生亲属中。本项目包括两个部分：（1）Sh 3的图位克隆和（2）阐明水稻驯化过程。在第（1）项中，高分辨率定位使我们能够将Sh 3的基因组区域缩小到小于5 kb。在该区域预测有一个候选ORF。然而，转化和测序分析显示ORF不是Sh 3。我们彻底审查了结果， ...更多信息 在第（2）项中，连锁作图显示所有测试的野生种都具有Sh 3。虽然O.glaberrima保留了功能性Sh 3等位基因，但它也携带隐性sh 9（iSh 3）基因。尽管有功能性Sh 3的背景，但隐性sh 9的纯合子表现出非破碎性。Sh 3和sh 9的高分辨率定位表明，这两个基因紧密连锁，但在不同的位点。此外，O.glaberrima具有可能导致（1）中出现的ORF中的提前终止密码子的核苷酸变化。本研究的结果如下：-：Sh 3基因的功能丧失与水稻的驯化密切相关。-：非破碎O.glaberrima保留了功能性Sh 3等位基因。鉴定了与Sh 3紧密连锁的sh 9基因。sh 9基因的功能缺失可能是光壳稻驯化的原因之一，我们设想通过分离Sh 3和sh 9基因，从核苷酸水平阐明水稻驯化的机制。少

项目成果

Nagai et al.: "A seed shattering gene, Sh3, and its inhibitor gene found in an African rice, Oryza glaberrima Steud."Rice Genetics Newsletter. 19. 76-77 (2002)

Nagai 等人：“在非洲水稻 Oryza glaberrima Steud 中发现了一种种子落粒基因 Sh3 及其抑制基因。”水稻遗传学通讯。

Y.S.Nagai, K.Doi, A.Yoshimura: "A seed shattering gene, Sh3, and its inhibitor gene found in an African rice, Oryza glaberrima Steud."Rice Genetics Newsletter. 19. 76-77 (2002)

Y.S.Nagai、K.Doi、A.Yoshimura：“在非洲水稻 Oryza glaberrima Steud 中发现了一种种子落粒基因 Sh3 及其抑制基因。”水稻遗传学通讯。

Nagai et al.: "A seed shattering gene, Sh3, and its inhibitor gene found in an African cultivated rice, Oryza glaberrima Steud"Rice Genetics Newsletter. 19(in press). (2002)

Nagai 等人：“在非洲栽培稻 Oryza glaberrima Steud 中发现的落粒基因 Sh3 及其抑制基因”水稻遗传学通讯。

Nagai et al.: "Sh3, a gene for seed shattering, commonly found in wild rices"Rice Genetics Newsletter. 19(in press). (2002)

Nagai 等人：“Sh3，一种种子落粒基因，常见于野生稻中”水稻遗传学通讯。

Nagai et al.: "Sh3, a gene for seed shattering, commonly found in wild rices"Rice Genetics Newsletter. 19. 74-76 (2002)

Nagai 等人：“Sh3，一种种子落粒基因，常见于野生稻中”水稻遗传学通讯。