EVALUATION AND DEVELOPMENT OF RICE BREEDING METHOD USING DNA MARKERS.

使用 DNA 标记的水稻育种方法的评价和开发。

• 批准号: 07556133
• 负责人: YOSHIMURA Atsushi
• 金额: $ 4.93万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07556133/
• 关键词: Rice; QTL analysis; recombinant inbred lines (RI lines); RFLP marker; breeding method; rapid advenced generation; RAPD marker; population

Using recombinant inbred lines (RI lines), F2 population and backcross population, QTL analysis for several traits, such as heading date, culm length, panicle length and etc., was performed. Many putative QTLs for some traits were detected. In QTL analysis specially for heading date, repeated and detailed examination was done. In addition, the linkage and substitution mapping of QTLs for heading date were done using the derivatives of the chromosome segment substitution and near-isogenic lines and gave the clear information about the QTLs.The rapid advanced generation at Okinawa in rice cross breeding was evaluated through monitoring the RFLP gene frequency in F3, F4 and F5 generations from the cross of Asominori and IR24. Section pressure near the region of semidwarf gene (sd1) was apparently observed.

利用重组自交系（RI系）、F2群体和回交群体，对抽穗期、秆长、穗长等性状进行了QTL分析，被执行。检测到多个可能的QTL。在抽穗期QTL分析中，进行了反复而详细的检测。利用染色体片段置换衍生系和近等基因系对抽穗期QTL进行了连锁和置换定位，明确了QTL的定位信息，并通过对Asominori与IR24杂交F3、F4和F5代RFLP基因频率的监测，对水稻杂交育种中冲绳的快速高代进行了评价。在半矮秆基因（sd1）附近观察到明显的断面压力。

项目成果

Tsunematsu, H.et al.: "RFLP framework map using recombinant inbred lines in rice." Breeding Science. 46. 279-284 (1996)

Tsunematsu, H.et al.：“使用水稻重组自交系的 RFLP 框架图。”

Yano, M.et al.: "Identification of quantitative trait loci controlling heading date in rice using a high-density Iinkage map." Theoretical and Applied Genetics. 95. 1025-1032 (1997)

Yano, M.et al.：“使用高密度连锁图识别控制水稻抽穗日期的数量性状基因座。”

Yamamoto, T., T.Sasaki and M.Yano: "Genetic analysis of spreading stub using indica/japonica backcrossed progenies in rice" Breeding Science. 47. 141-144 (1997)

Yamamoto, T.、T.Sasaki 和 M.Yano：“利用水稻籼稻/粳稻回交后代进行传播残株的遗传分析”育种科学。

Yoshimura, A., J.X.Lei, T.Matsumoto, H.Tsunematsu, S.Yoshimura, N.Iwata, M.R.Baraoidan, T.W.Mew and R.J.Nelson: "Analysis and pyramiding of bacterial blight resistance genes in rice by using DNA markers" Rice Genetics III. 577-581 (1996)

Yoshimura, A.、J.X.Lei、T.Matsumoto、H.Tsunematsu、S.Yoshimura、N.Iwata、M.R.Baraoidan、T.W.Mew 和 R.J.Nelson：“利用 DNA 标记对水稻白叶枯病抗性基因进行分析和金字塔化” 水稻