DEVELOPMENT AND EVALUATION OF CHROMOSOME SUBSTITUTION LINES INTROGRESSED FROM ORYZA GLABERRIMA -A TRIAL FOR REVERSED TRAIT ANALYSIS-

利用水稻（Oryza sativa）杂交的BC1F1群体。台中65)/O。glaberrima (IRGC104038)；构建了由101个标记组成的RFLP连锁图谱。标记的排列和标记间的距离与以往报道的稻种内杂交RFLP连锁图谱基本一致。在6号染色体上发现变异增加等位基因频率的现象。对BC2F1群体的抽穗日期和花粉不育性进行了高级回交QTL分析。抽穗日期的QTL在第1、6和10号染色体上进行了检测。1号和6号染色体上的等位基因分别延迟了抽穗日期。6号染色体QTL上的等位基因对早熟抽穗有影响。在第3、7、10染色体上检测到花粉不育QTL。glaberrima等位基因在每个QTL上都增加了花粉不育性，其中第10染色体上的glaberrima等位基因具有较强的功能。到目前为止，标记辅助选择一直进行到BC3F1代，以形成染色体替代系。目前正在对900株BC3F2植物进行筛选，以建立染色体替代系。

Using a BC1F1 population from the cross of Oryza sativa (cv.Taichung 65) /O.glaberrima (IRGC104038) //O.sativa, the RFLP linkage map being consisted of 101 markers was construced. The arrangement of markers and the map distances between markers were almost the same as those of the RFLP linkage maps from intraspecific crosses of Oryza sativa previously reported. The distortion increasing the allele frequency of O.glaberrima was found on chromosime 6.Advanced backcross QTL analysis was performed in BC2F1 population with respect to heading date and pollen sterility. The QTL for heading date were detected on chromosomes 1,6 and 10. The alleles from O.glaberrima at QTL on chromosomes 1 and 6 delayd the heading date, respectively. However, the allele from O.glaberrima at QTL on chromosome 6 affected early heading. The QTL for pollen sterility was detected on chromosomes 3,7 and 10. The glaberrima allele at each QTL increased pollen sterility and that on chromosome 10 appeared to have strong function.So far, marker assisted selection was done up to the generations of BC3F1 to develop the chromosome substitution lines. The selection from 900 plants in BC3F2 is now under way to establish the chromosome substitution lines.