DEVELOPMENT AND EVALUATION OF CHROMOSOME SUBSTITUTION LINES INTROGRESSED FROM ORYZA GLABERRIMA -A TRIAL FOR REVERSED TRAIT ANALYSIS-

以台中65/光叶野生稻（IRGC 104038）的BC 1F 1群体//O.sativa，构建了由101个标记组成的RFLP连锁图谱。标记的排列和标记间的距离与已报道的水稻种内杂交的RFLP图谱基本一致。在BC 2F 1群体中对抽穗期和花粉育性进行了进一步的回交QTL分析。在第1、6、10染色体上检测到抽穗期QTL。光果野生稻位于第1和第6染色体上的QTL分别延迟抽穗期。而第6染色体上来自光萼野生稻的QTL对早抽穗有影响。在第3、7和10染色体上检测到花粉不育的QTL。每个QTL上的glaberrima等位基因都能提高花粉育性，其中第10染色体上的glaberrima等位基因表现出较强的功能，目前已对BC 3F 1代进行了标记辅助选择，以培育染色体代换系。目前正在从900株BC 3F 2植株中进行选择，以建立染色体代换系。

Using a BC1F1 population from the cross of Oryza sativa (cv.Taichung 65) /O.glaberrima (IRGC104038) //O.sativa, the RFLP linkage map being consisted of 101 markers was construced. The arrangement of markers and the map distances between markers were almost the same as those of the RFLP linkage maps from intraspecific crosses of Oryza sativa previously reported. The distortion increasing the allele frequency of O.glaberrima was found on chromosime 6.Advanced backcross QTL analysis was performed in BC2F1 population with respect to heading date and pollen sterility. The QTL for heading date were detected on chromosomes 1,6 and 10. The alleles from O.glaberrima at QTL on chromosomes 1 and 6 delayd the heading date, respectively. However, the allele from O.glaberrima at QTL on chromosome 6 affected early heading. The QTL for pollen sterility was detected on chromosomes 3,7 and 10. The glaberrima allele at each QTL increased pollen sterility and that on chromosome 10 appeared to have strong function.So far, marker assisted selection was done up to the generations of BC3F1 to develop the chromosome substitution lines. The selection from 900 plants in BC3F2 is now under way to establish the chromosome substitution lines.