Toward the reconstruction of genetics and breeding of bacterial blight resistance in ric

水稻抗白叶枯病遗传育种的重建

• 批准号: 20380007
• 负责人: YOSHIMURA Atsushi
• 金额: $ 11.23万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20380007/
• 关键词: 植物; 育種学; 病理学; ゲノム; 細菌病; イネ白葉枯病; R遺伝子; 非病原性遺伝子; マップベースクローニング; 感染生理; 新抵抗性遺伝子資源; 野生イネコアコレクション; マッピング

Two kinds of experiments were conducted in this study ;1. Map-based cloning of Xa7 gene : The region in which Xa7 was located was narrowed down to the 128kb region on the donor DNA. There are 20 kinds of predicted genes in the region, where previously-known R genes are not included. Transformation of 6 subdivided DNA fragments was performed, but it was difficult to check gain-of function because the recipient used for transformation showed somewhat resistance. The experiment of complementation test would be done in IRRI, because IRRI can handle the many T1 plants for inoculation test.2. Bacterial blight resistance in wild species of rice and gene mapping of Bacterial blight resistance : We evaluated about 300 strains of wild species. One-third of them showed the resistance to two Japanese races 1 and 3, demonstrating that wild species are apparently important materials as gene resource for bacterial blight resistance. The mapping study of Xa11 was published in JARQ.

本研究进行了两种实验：1. Xa 7基因的图位克隆：将Xa 7基因定位的区域缩小到供体DNA上的128 kb区域。在该区域中有20种预测基因，其中不包括以前已知的R基因。进行了6个细分DNA片段的转化，但由于用于转化的受体表现出一定的抗性，因此难以检查功能获得性。互补试验在IRRI进行，因为IRRI可以处理大量的T1代植株进行接种试验.水稻野生种的白叶枯病抗性和白叶枯病抗性基因定位：我们评估了大约300个野生种菌株。有1/3的野生种对日本1号和3号生理小种表现抗性，表明野生种是重要的抗白叶枯病基因资源。Xa 11的定位研究发表在JARQ上。

项目成果

Mapping of Bacterial Blight Resistance Gene Xa11 on Rice Chromosome 3

• DOI: 10.6090/jarq.43.221
• 发表时间: 2009-07-01
• 期刊: JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY
• 影响因子: 0.4
• 作者: Goto, Takahiro;Matsumoto, Tadayuki;Yoshimura, Atsushi
• 通讯作者: Yoshimura, Atsushi

Mapping of Bacterial Blight Resistance Gene Xall on Rice Chromosome 3

水稻3号染色体上白叶枯病抗性基因Xall的定位

• 发表时间: 2009
• 期刊: JARQ 43
• 作者: T.GOTO;T.MATSUMOTO;N.FURUYA;K.TSUCHIYA;A.YOSHIMURA
• 通讯作者: A.YOSHIMURA

イネ白葉枯病抵抗性遺伝子Xallのマッピング

水稻白叶枯病抗性基因Xall的定位

• 发表时间: 2008
• 作者: 後藤高弘;松本忠之;古屋成人;土屋健一;吉村 淳
• 通讯作者: 吉村 淳