Cellulase System and Microflora on Cellulose Materials in Soil.

在本研究的第一部分中，采用刚果红染色技术对土壤中好氧纤维素分解者进行计数。该方法较好地克服了原有CMC制版方法存在的重叠、清晰度差、清带消失等缺点。在第二部分，研究了施用杀菌剂百菌清对土壤中纤维素在25和13价位下的分解过程的影响。农药引起了纤维素分解的阻滞和纤维素片上真菌菌群的变化。土壤中土壤枯丝核菌（Rhizoctonia solani）的相对优势度和频率在施用土壤中较高，在施用土壤中较低。研究了一种从土壤中纤维素材料中提取游离纤维素酶的简便方法。用聚丙烯酰胺凝胶电泳法对提取的纤维素酶进行分析。在不同的孵育温度和不同的农药用量下，检测到几种同工酶模式，即纤维素片上微真菌菌群的差异。这些结果部分发表在《土壤科学》杂志上。植物减轻。[j] .中国农药科学，1993,19(3):361-365。

In the first part of this study, a method in which the Congo red staining technique was applied was adopted for enumerating aerobic cellulose decomposers in soil. By this new method, the shortcomings of the original method of CMC plate such as overlapping, lack of definition and disappearance of clear zones were overcome considerably.In the next part, the effect of a fungicide, chlorothalonil application on cellulose decomposing process in soil at 25ﾟC and 13ﾟC.The pesticide caused the retardation of cellulose decomposition and the shifts in the fungal flora on the cellulose sheets. Relative dominance and frequency of a fungal species, Rhizoctonia solani, at 13ﾟC were high in the control soil but low in the pesticide applied soil.The simple method which can be used to extract free cellulases from cellulose materials in soil was developed. The cellulases, especially CMCases to be extracted by the method were analyzed by poly acrylamide gel electrophoresis. Several isozyme patterns were detected in compliance with the difference of incubation temperature and the pesticide dosage, that is, the difference of microfungal flora on the cellulose sheet.These results in part were published in Soil Sci. Plant Nutr., 39,361-365,1993, J.Pestiscide Sci. 18,225-230,285-292 (1993).