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Expression and localization of intestine-derived faty acid-binding proteins in the rat

大鼠肠源性脂肪酸结合蛋白的表达和定位

基本信息

批准号：
04670009
负责人：
ISEKI Shoichi
金额：
$ 1.28万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

A family of homologous, low-molecular-weight cytosolic proteins with high affinity for fatty acidsis expressed in mammalian tissues and called fatty acid-binding protein (FABP). Rat small intestine is knownto express several distinct FABPs, including newly-discovered intestinal 15 kDa protein (1-15P). We studied the expresion and localization of I-15P in the entire rat body, using the immunocytochemistry to localize protein and in situ hybridization to localize mRNA.Both techniques demonstrated the expression of I-15P in the enterocytes of ileum, luteal cells of ovary and a subpopulation of steroid-endocrine cells of adrenal gland. Immuno-electron microscopy further demonstrated that I-15P is localized in both the cytoplasmic and nuclear matrix regions of above cells. These results suggested the roles of I-15P not only in the transport of bile salts by ileal epithelium but also in the metabolisms of certain steroid hormones. Next, the immuohistohemical localizations of two types of FABPs, i.e., I-15P and heart FABP (H-FABP), were examined in the developing rat overies and in the rat overies induced to ovulate by gonadotropins. In the postnatal development of ovary, I-15P-immunoreactivity was localized temporarilyin a small subpopulation of theca/interstitial cells from 2 weeks to 4 weeks and then in the luteal cells from 5 weeks, in coincidence with the onset of cycles, into adulthood. In the immature (3 week old) ovaries inducedto ovulate by treatment with pregnant mare serum gonadotropin and human chorionic gonadotropin, I-15P-immunoreactivity first appeard in the luteinizing granulosa cells of large preovulatory follicles. In contrast, H-FABP-immunoreactivity was present temporarily in the follicular epithelial (granulosa) cells during the initial 2 weeks and thereafter in the theca/interstitial cells from 2 weeks postpartum into adulthood. These results suggested that two types of FABPs are expressed in distinct steroid cell populations of rat ovary, implecatin

一个同源、低分子量胞质蛋白家族，对脂肪酸中毒具有高亲和力，在哺乳动物组织中表达，称为脂肪酸结合蛋白 (FABP)。已知大鼠小肠表达几种不同的 FABP，包括新发现的肠道 15 kDa 蛋白 (1-15P)。我们研究了 I-15P 在整个大鼠体内的表达和定位，使用免疫细胞化学定位蛋白质和原位杂交定位 mRNA。两种技术都证明了 I-15P 在回肠肠上皮细胞、卵巢黄体细胞和肾上腺类固醇内分泌细胞亚群中的表达。免疫电镜进一步证明I-15P定位于上述细胞的细胞质和核基质区域。这些结果表明 I-15P 不仅在回肠上皮转运胆汁盐中发挥作用，而且在某些类固醇激素的代谢中也发挥作用。接下来，在发育中的大鼠上皮和促性腺激素诱导排卵的大鼠上皮中检查了两种类型 FABP（即 I-15P 和心脏 FABP (H-FABP)）的免疫组织化学定位。在卵巢的出生后发育过程中，I-15P 免疫反应性在 2 周至 4 周内暂时位于卵泡膜/间质细胞的一小部分亚群中，然后从 5 周开始位于黄体细胞中，与周期开始一致，直到成年。在用孕马血清促性腺激素和人绒毛膜促性腺激素处理诱导排卵的未成熟（3周龄）卵巢中，I-15P-免疫反应性首先出现在大排卵前卵泡的黄素化颗粒细胞中。相比之下，H-FABP 免疫反应性在最初 2 周内暂时存在于滤泡上皮（颗粒）细胞中，此后从产后 2 周到成年期间存在于卵泡膜/间质细胞中。这些结果表明两种类型的 FABP 在大鼠卵巢的不同类固醇细胞群中表达，