Expression and localization of vascular endothelial growth factor in the endocrine system

血管内皮生长因子在内分泌系统中的表达和定位

• 批准号: 09670006
• 负责人: ISEKI Shoichi
• 金额: $ 2.05万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670006/
• 关键词: vascular endothel-ial growth factor; VEGF; endocrine cells; mast cells; immunohistochemistry; rat; 内分泌腺

Vascular endothelial growth factor (VIEGE) is a potent angiogenic mitogen and also increases vascular permeability. Immunohistochemical localization of VEGF in the entire body of healthy adult rats was investigated at light and electron microscopic levels using a specific antibody. Intense to moderate immunoreactivities for VEGF were detected in certain endocrine cell types, namely, the parafollicular cell of thyroid gland, B cell of endocrine pancreas, N cell of adrenal medulla and a minority of the TSH cell of anterior pituitary gland. At the ultrastructural level, VEGF immunoreactivity was localized exclusively in the secretory granules of all immunopositive endocrine ells. These results provided immunohistochemical evidence for the occurrence of VIEGF in subsets of endocrine cells of normal adult rats, suggesting that these secretory cells regulate local vascular permeability through a paracrine action of VEGF (Reference 1).The results also showed solitary cells with strong VEGF im … More munoreactivity scattered in the epithelium of the respiratory tract as well as in the lamina propria and epithelium of the small and large intestines. By ultrastructural features of their large cytoplasmic granules, these VEGF-positive cells were identified as globule leukocytes (GL), also called mucosal mast cells (MMC). The immunoreactivity was localized exclusively in the cytoplasmic granules of GIIMMC.When examined in serial sections, GL/MMC in the respiratory and digestive tracts showed only weak reactivity to anti-histamine antibody. In contrast, connective tissue mast cells (CTMC), which were located in the submucosa of digestive tract and in the connective tissue of respitratory tract and a variety of other organs, were intensely immunopositive for histamine, whereas they showed no reactivity for VBGF.The specific appearance of VEGF immunoreactivity in GL/MMC suggested that this cell type is involved in the paracrine regulation of the permeability of nearl5y microvessels, and that VEGE can be used as a histochemical marker to distinguish GL/MMC from CTMC(Reference2). Less

血管内皮生长因子（VIEGE）是一种有效的促血管生成的有丝分裂原，也增加血管通透性。VEGF在健康成年大鼠全身的免疫组织化学定位进行了研究，在光镜和电子显微镜水平，使用特异性抗体。在某些内分泌细胞类型中检测到VEGF的强至中等免疫反应性，即甲状腺的滤泡旁细胞、内分泌胰腺的B细胞、肾上腺髓质的N细胞和少数垂体前叶的TSH细胞。在超微结构水平上，VEGF免疫反应只局限于所有免疫阳性内分泌细胞的分泌颗粒。这些结果为正常成年大鼠内分泌细胞亚群中VIEGF的存在提供了免疫组化证据，表明这些分泌细胞通过VEGF的旁分泌作用调节局部血管通透性（参考文献1）。 ...更多信息 免疫反应性分散在呼吸道上皮以及小肠和大肠的固有层和上皮中。根据其胞质大颗粒的超微结构特征，这些VEGF阳性细胞被鉴定为球白细胞（GL），也称为粘膜肥大细胞（MMC）。免疫反应仅定位于GIIMMC的胞浆颗粒，连续切片观察，呼吸道和消化道的GL/MMC对抗组胺抗体呈弱反应。而位于消化道粘膜下层、呼吸道结缔组织及其它器官的结缔组织肥大细胞（CTMC）对组胺呈强阳性反应，而对VBGF无反应。GL/MMC中VEGF免疫反应的特异性出现提示该细胞参与近15年微血管通透性的旁分泌调节。VEGE可作为鉴别GL/MMC与CTMC的组织化学标志物（参考文献2）。少

项目成果

Fan,L and Iseki,S: "Immunohistochemical localization of vascular endothelial growth factor in the endocrine glands of the rat" Archives of Histology and Cytology. 61・1. 17-28 (1998)

Fan, L 和 Iseki, S：“大鼠内分泌腺中血管内皮生长因子的免疫组织化学定位”，组织学和细胞学档案 61・1（1998）。

Fan,L. & Iseki,S.: "Immunohistochemical localization of vascular endothelial growth factor in the endocrine glands of the rat" Arch.Histal.Cytol.61(1)(印刷中). (1998)

Fan, L. 和 Iseki, S.：“大鼠内分泌腺中血管内皮生长因子的免疫组织化学定位”Arch.Histal.Cytol.61(1)（出版中）。

Fan,L.and Iseki,S.: "Immunohistochemical localization of vascular endothelial growth factor in the globule leukocyte / mucosal mast cell of the rat" Histochem.Cell Biol.111・1. 13-21 (1999)

Fan, L. 和 Iseki, S.：“大鼠球状白细胞/粘膜肥大细胞中血管内皮生长因子的免疫组织化学定位”Histochem.Cell Biol.111・1（1999）。

Fan,L.and Iseki,S.: "Immunohistochemical localization of vascular endothelial growth factor in the endocrine glands of the rat" Arch,Histol.Cytol.61・1. 17-28 (1998)

Fan, L. 和 Iseki, S.：“大鼠内分泌腺中血管内皮生长因子的免疫组织化学定位”Arch，Histol 61·1（1998）。

Fan,L and Iseki,S: "Immunohistochemical localization of vascular endothelial growth factor in the globule leukocyte/mucosal mast cells of the rat respiratory and digestive tracts" Histochemistry and Cell Biology. 111・1. 13-21 (1999)

Fan, L 和 Iseki, S：“大鼠呼吸道和消化道球状白细胞/粘膜肥大细胞中血管内皮生长因子的免疫组织化学定位”《组织化学和细胞生物学》111・1（1999）。