权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The mechanism of androgen-dependent differentiation of the duct system in the submandibular gland

颌下腺导管系统雄激素依赖性分化机制

基本信息

批准号：
15590156
负责人：
ISEKI Shoichi
金额：
$ 2.3万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

In regard to the differentiation of rodent submandibular glands (SMG), we have obtained the following results :1)In the mouse SMG, the expression of the transcription factor JunD is localized to the duct system and much more abundant in the female than in the male gland. During the postnatal development and testosterone-induced GCT cell differentiation, JunD expression is temporarily induced in the nuclei of striated duct cells and dispappeares as they differentiated into GCT cells (Reference 1).2)The expression and localization of menin, a nuclear protein that binds JunD, showed the same pattern in the mouse SMG as that of JunD, suggesting that JunD-menin complex is responsible for the duct differentiation.3)In the SMG of hypophysectomized rats, the induction of the protease inhibitor cystatin S by the b-adrenergic agonist isoproterenol is much less than that in the control. Administration of one of the pituitary-dependent hormones, i.e., testosterone, estradiol, dexamethasone and thyroxine, together with isoproterenol results in marked recovery of cystatin S expression (Reference 2).These results suggest the role of cross-talks between multiple signaling pathways, including that of androgens, in the differentiation of rodent SMG.4)We have cloned a novel animal lectin specifically expressed in the rat sublingual gland and named it STAMP (sublingual gland acinar membrane protein). SLAMP is localized to the membrane of ER-Golgi intermediate compartment (ERGIC), suggesting its role in the early secretory pathway of mucin glycoproteins.

关于啮齿类下颌下腺（SMG）的分化，我们得到了以下结果：1）在小鼠SMG中，转录因子JunD的表达定位于导管系统，并且雌性下颌下腺的表达量远高于雄性腺体。在出生后发育和睾酮诱导的 GCT 细胞分化过程中，JunD 表达在横纹管细胞核中暂时被诱导，并在分化为 GCT 细胞时消失（参考文献 1）。2）与 JunD 结合的核蛋白 menin 的表达和定位在小鼠 SMG 中表现出与 JunD 相同的模式，表明 JunD-menin 复合物负责导管分化。3）在在垂体切除的大鼠中，b-肾上腺素能激动剂异丙肾上腺素对蛋白酶抑制剂半胱氨酸蛋白酶抑制剂S的诱导作用比对照组小得多。给予一种垂体依赖性激素，即睾酮、雌二醇、地塞米松和甲状腺素，与异丙肾上腺素一起使用，可导致胱抑素 S 表达显着恢复（参考文献 2）。这些结果表明，包括雄激素在内的多种信号通路之间的串扰在啮齿动物 SMG 的分化中发挥着作用。4）我们专门克隆了一种新型动物凝集素。在大鼠舌下腺中表达，并将其命名为 STAMP（舌下腺腺泡膜蛋白）。 SLAMP 定位于 ER-高尔基体中间室 (ERGIC) 的膜，表明其在粘蛋白糖蛋白的早期分泌途径中的作用。