Responses of subfornical organ neurons to cardiovascular-related peptides and their intracellular second messenger system

穹窿下器官神经元对心血管相关肽及其细胞内第二信使系统的反应

• 批准号: 04670104
• 负责人: INENAGA Kiyotoshi
• 金额: $ 1.28万
• 依托单位: University of Occupational & Environmental Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670104/
• 关键词: Subfornical Organ; Angiotensin; Gamma-amino butyric acid; Glutamate; Slice; Intracellular recording; Anomalous rectification; アンヂオテンシン; ガンマ-アミノ酪酸; 脳弓下器管; GABA

The subfornical organ(SFO) takes an important role in central cardiovascular control and drinking behavior, and modulates release of arginine vasopressin and oxytocin. This study is designed to elucidate(1) basic electrophysiological properties of the neurons,(2) synaptic inputs to the SFO,(3) effects of angiotensin II(AII) and(4) effects of gamma-animobutyric acid (GABA) on the SFO neurons. For these purposes, slice preparation of the SFO of rats and conventional intracellular recordings were used. Half of SFO neurons fired spontaneously and most neurons had anomalous inward rectification and low threshold calcium spike. The SFO received excitatory and inhibitory synaptic inputs, through glutamic acid and GABA, respectively. All excited most SFO neurons with either closing of potassium channels or opening of non-selective cation channels. The blood-born All seems to affect the SFO neurons, but not the neuronal All. GABA sends inhibitory synaptic inputs through GABAA receptors and activates chloride channels.

穹窿下器（SFO）在中枢心血管控制和饮酒行为中起重要作用，并调节精氨酸加压素和催产素的释放。本研究旨在阐明（1）神经元的基本电生理特性，（2）SFO的突触输入，（3）血管紧张素II（AII）的作用，（4）γ-氨基丁酸（GABA）对SFO神经元的作用。为了这些目的，使用大鼠SFO的切片制备和常规细胞内记录。半数SFO神经元自发放电，大多数神经元有异常内向整流和低阈值钙峰。SFO分别通过谷氨酸和GABA接受兴奋性和抑制性突触输入。所有兴奋大多数SFO神经元关闭钾通道或开放的非选择性阳离子通道。血液中的All似乎影响SFO神经元，但不影响神经元的All。GABA通过GABAA受体发送抑制性突触输入并激活氯离子通道。

项目成果

山下 博、稲永 清敏 長友 敏寿: "視床下部ー下重体系におけるインビトロ研究の現状と展開(II)ースライスパッチ法ー" ブレインサイエンス. 3. 97-101 (1992)

Hiroshi Yamashita、Kiyotoshi Inanaga 和 Toshihisa Nagatomo：“下丘脑下移植系统体外研究的现状和发展（II）-切片补片法”《脑科学》3. 97-101（1992）。

Inenaga, K.: "Kappa-selective agonists decrease postsynaptic potentials and calcium components of action potentioals in the supraoptic nucleus of rat hypothalamus in vitro." Neuroscience. 58. 331-340 (1993)

Inenaga, K.：“Kappa 选择性激动剂可在体外降低大鼠下丘脑视上核的突触后电位和动作电位的钙成分。”

Yamamoto,S.: "The actions of endothelin on single cells in the anteroventral third ventricular region and supraoptic nucleus in rat hypothalamic slices." J.Neuroendocrinology. 5. 427-434 (993)

Yamamoto,S.：“内皮素对大鼠下丘脑切片中前腹侧第三脑室区域和视上核的单个细胞的作用。​​”

Inenaga,K.: "Kappa-selective agonists decrease postsynaptic potentials and calcium components of action potentials in the supraoptic nucleus of rat" Neuroscience. 58. 331-340 (1994)

Inenaga,K.：“Kappa 选择性激动剂降低大鼠视上核的突触后电位和动作电位的钙成分”《神经科学》。

Tribollet,E.,Li,Z.,Inenaga,K.,et al: "Functional neuronal binding sites for oxytocin in the ventromedial hypothalamus of the guinea-pig after gonadectomy." Brain Research. 588. 346-350 (1992)

Tribollet，E.，Li，Z.，Inenaga，K.，等人：“性腺切除术后豚鼠腹内侧下丘脑中催产素的功能性神经元结合位点。”