Analysis of thirst mechanisms: osmosis and sodium sensing

口渴机制分析：渗透和钠传感

• 批准号: 16591862
• 负责人: INENAGA Kiyotoshi
• 金额: $ 2.3万
• 依托单位: Kyushu Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591862/
• 关键词: Dry mouth; Osmotic perception; Sodium sensing; Nax receptor; Drinking; Slice patch; Mouse

Thirst sensation is induced by stimulating the thirst center, including the circumventricular organs. The electrical activities were recorded from mouse slice preparation, by using patch clam recordings. SFO neurons were depolarized by hypertonic stimulation, under current clamp mode. The depolarization amplitude in the 30 mM NaC1 stimulation was large than that in the 60 mM mannitol stimulation. The same stimuli induced inward currents under voltage clamp mode. The inward currents were also larger in the NaC1 than mannitol. As for postsynaptic currents, the frequencies of EPSCs were increased and those of IPSCs were decreased by the same hypertonic stimuli. The increment and decrement in synaptic currents were also larger in the NaC1 than mannitol. The amplitude of their synaptic currents was unaffected. There suggest that SFO neurons sense to both hypertonicity and sodium ions and the sodium sensing is superior to the osmotic perception.

口渴的感觉是由刺激口渴中心，包括脑室周围器官引起的。在制备小鼠脑片的基础上，采用斑片法记录其电活动。在电流钳模式下，高渗刺激使SFO神经元去极化。30 mM氯化钠刺激的去极化幅度大于60 mM甘露醇刺激的去极化幅度。在电压钳模式下，相同的刺激产生内向电流。NaC_1的内向电流也大于甘露醇。对于突触后电流，相同的高张刺激可使EPSCs频率增加，IPSCs频率降低。Nc1区突触电流的增减幅度也大于甘露醇组。它们的突触电流的幅度没有受到影响。提示SFO神经元对高渗和钠离子均有感觉，钠离子感觉优于渗透压感觉。

项目成果

Transient outward K+ currents in rat dissociated subfornical organ neurones and angiotensin II effects.

大鼠分离穹窿下器官神经元中的瞬时外向 K 电流和血管紧张素 II 效应。

• 发表时间: 2005
• 期刊: Journal of Physiology 568(3)
• 作者: K.Ono;T.Toyono;E.Honda;K.Inenaga
• 通讯作者: K.Inenaga

Central nicotinic stimulation reduces vascular conductance in the gingival in anesthetized rats.

中枢烟碱刺激降低了麻醉大鼠牙龈中的血管电导。

• 发表时间: 2005
• 期刊: J Periodontal Research 40
• 作者: Nakamura T;Ono K;Honda E;Yokota M;Inenaga.
• 通讯作者: Inenaga.

Activation of subfomical organ neurons in rats through pre-and post-synaptic

通过突触前和突触后激活大鼠窝下器官神经元

• 期刊: Am. J. Physiol. (in press)
• 作者: Honda E;Ono K;Kataoka S;Inenaga K.
• 通讯作者: Inenaga K.

The functional evaluation of salivary glands using dynamic MR sialograph following citric acid stimulation : a preliminary study.

柠檬酸刺激后使用动态 MR 唾液记录仪对唾液腺进行功能评估：初步研究。

• 发表时间: 2005
• 期刊: Oral Surg Oral Med Oral Pathol Oral Radiol Endod 100
• 作者: Morimoto Y;Ono K;Tanaka T;Kito S;Inoue H;Shinohara Y;Yokota M;Inenaga K;Ohba T.
• 通讯作者: Ohba T.

Down regulation of AQP2 expression in the kidney of polydipsic STR/N mice.

烦渴 STR/N 小鼠肾脏 AQP2 表达下调。

• 发表时间: 2005
• 期刊: Am. J. Physiol. 290
• 作者: Tsumura K;Li X;Murdiastuti K;Nahid M P;Akamatsu T;Yao C;Kanamori N;Inenaga K;Yamashita H;Hosoi K.
• 通讯作者: Hosoi K.