Induction mechanism of oral dryness by the sialogogue pilocarpine

唾液酸毛果芸香碱诱导口腔干燥的机制

• 批准号: 20592178
• 负责人: INENAGA Kiyotoshi
• 金额: $ 3.08万
• 依托单位: Kyushu Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592178/
• 关键词: ピロカルピン; セビメリン; 唾液分泌; 口渇; 昇圧; 昇圧反応; 喉の渇き; 口腔乾燥; ムスカリン受容体; 口渇中枢; 催唾剤

Pilocarpine and cevimeline induce salivary secretion and get wet in the oral cavity. We showed that intraperitoneally and intracerebroventricularly injected pilocarpine elicited water intake in rats and the induced behavior was suppressed by intracerebroventricularly preadministered atropine. The same applications of pilocarpine increased the number of c-Fos positive neurons in the subfornical organ, median nucleus of preoptic area, and organum vasculosum of lamina terminalis, which may constitute the thirst center in the brain. Pilocarpine directly affected and depolarized neurons in the subfornical organ of slice preparations. On the other hand, intraperitoneally injected cevimeline did not increase water intake but rather suppressed angiotensin II-induced water intake. Pilocarpine and cevimeline increased intracellular calcium concentration in acinar cells of the salivary gland in vitro preparations. The increment of intracellular calcium concentration with in vitro application was rather short-lasting than with in vivo application. Thus, pilocarpine and cevimeline show actions on acinar cells of the salivary gland through the same intracellular second messenger system. From these results, it is suggested that pilocarpine and cevimeline have different actions between the central nervous system and the peripheral organs.

毛果芸香碱和西维美林诱导唾液分泌并在口腔中润湿。我们发现，腹腔内和侧脑室注射毛果芸香碱引起大鼠的饮水量和诱导的行为被抑制的侧脑室预先给药阿托品。结果表明，在穹窿下器、视前区正中核和终板血管器内c-Fos阳性神经元的数量明显增加，这些部位可能构成了脑内的口渴中枢。毛果芸香碱直接影响和去极化神经元在穹窿下器官的切片制备。另一方面，腹腔注射西维美林并没有增加水的摄入，而是抑制血管紧张素II诱导的水的摄入。毛果芸香碱和西维美林增加细胞内钙离子浓度在腺泡细胞的唾液腺体外制剂。细胞内钙离子浓度的增加，在体外应用是相当短暂的，而不是在体内应用。因此，毛果芸香碱和西维美林通过相同的细胞内第二信使系统对唾液腺的腺泡细胞显示作用。结果提示，毛果芸香碱和西维美林对中枢和外周器官的作用不同。

项目成果

Mechanism of drinking induced by the sialogogue pilocarpine in rats

唾液酸毛果芸香碱诱导大鼠饮酒的机制

• 发表时间: 2009
• 作者: 稲永清敏;小野堅太郎;宮原宣高;若林-佐藤奈緒
• 通讯作者: 若林-佐藤奈緒

ラット耳下腺細胞のコリン性・アドレナリン性刺激による多様な反応

大鼠腮腺细胞对胆碱能和肾上腺素能刺激的各种反应

• 发表时间: 2008
• 作者: 稲垣智浩;小野堅太郎;増田渉;細川隆司;稲永清敏
• 通讯作者: 稲永清敏

Effects of pilocarpine and cevimeline on Ca2+ mobilization in rat parotid acini and ducts.

毛果芸香碱和西维美林对大鼠腮腺腺泡和导管中 Ca2+ 动员的影响。

• DOI: 10.2152/jmi.56.375
• 发表时间: 2009
• 期刊: The journal of medical investigation : JMI
• 作者: K. Ono;T. Inagaki;Taichi Iida;R. Hosokawa;K. Inenaga
• 通讯作者: K. Inenaga

Nicotinic receptor agonist-induced salivation and its cellular mechanism in parotid acini of rats

• DOI: 10.1016/j.autneu.2011.01.003
• 发表时间: 2011-04-26
• 期刊: AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL
• 影响因子: 2.7
• 作者: Iida, Taichi;Ono, Kentaro;Inenaga, Kiyotoshi
• 通讯作者: Inenaga, Kiyotoshi

Mechanism of drinking behavior by central activation of nicotinic receptor in rat.

大鼠烟碱受体中枢激活的饮酒行为机制。

• 发表时间: 2009
• 作者: Inenaga K;Ono K;Miyahara N.
• 通讯作者: Miyahara N.