Induction of oral dryness and endocannabinoid

诱导口腔干燥和内源性大麻素

• 批准号: 23592743
• 负责人: INENAGA Kiyotoshi
• 金额: $ 3.41万
• 依托单位: Kyushu Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592743/
• 关键词: 内因性カンナビノイド; 脳弓下器官; 飲水行動; エタノール; のどの渇き; 飲水; ナトリウム摂取; パッチクランプ; γアミノ酪酸

This study was to clarify a relationship between endocannabinoid and thirst sensation. Male Wistar rats were used in the experiment. First, the effects of endocannabinoids on IPSCs of neurons of the subfornical organ (SFO), which is a thirst center of the brain, recorded from brain slice preparations were investigated with patch clamp method. The CB1 receptor agonist WIN 55,221-2 (WIN) decreased significantly the frequency but not the amplitude of IPSCs. The effect was disappeared with the CB1 antagonist. Next, the endocannabinoids on neural activity of the SFO were investigated with the multi-electrode array (MEA) method. Application of the endocannabinoid anandamide and WIN affected unit activities in only the small number of neurons. The i.c.v. injection of WIN had no effects on water and salt intakes. These suggest that while endocannabinoids affect GABA release in the SFO, they do not play any important role in induction of thirst sensation, if present, in the normal condition.

本研究旨在阐明内源性大麻素与口渴感之间的关系。实验中使用雄性Wistar大鼠。首先，用膜片钳方法研究了内源性大麻素对穹窿下器官（SFO）神经元IPSC的影响，SFO是大脑的口渴中心，记录自脑片制备物。CB 1受体激动剂WIN 55，221 -2（WIN）显著降低IPSC的频率，但不降低振幅。CB 1拮抗剂的作用消失。接下来，内源性大麻素对SFO的神经活动的多电极阵列（MEA）方法进行了研究。内源性大麻素anandamide和WIN的应用仅在少数神经元中影响单位活动。静脉注射WIN对水和盐的摄入量没有影响。这些表明，虽然内源性大麻素影响GABA释放的SFO，他们不起任何重要的作用，在诱导口渴的感觉，如果存在，在正常条件下。

项目成果

Neuronal effects of neurokinin B on the rat subfornical organ

神经激肽B对大鼠穹窿下器官的神经元作用

• DOI: 10.1097/wnr.0b013e3283469645
• 发表时间: 2011
• 期刊: Neuroreport
• 影响因子: 1.7
• 作者: Ono K;Asami R;Miyahara N;Nakanishi O;Inenaga K.
• 通讯作者: Inenaga K.

研究者総覧

研究员名单

Dopamine inhibits neuronal excitability in the rat subfornical organ

多巴胺抑制大鼠穹窿下器官神经元兴奋性

• 作者: Inenaga K;Miyahara N;Ono K.
• 通讯作者: Ono K.

Pre-and post-synaptic modulation by dopamine in rat subfornical organ neurons

多巴胺对大鼠穹窿下器官神经元的突触前和突触后调节

• 作者: Inenaga K;Miyahara N;Ono K;Hitomi S.
• 通讯作者: Hitomi S.

九州歯科大学・研究者総覧

九州齿科大学/研究员名单