ELECTROPHYSIOLOGICAL PROPERTIES AND OSMOSENSITIVITIES OF ISOLATED HYPOTHALAMIC NEURONS.

孤立的下丘脑神经元的电生理特性和渗透敏感性。

• 批准号: 63570072
• 负责人: INENAGA Kiyotoshi
• 金额: $ 1.34万
• 依托单位: UNIVERSITY OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH, SCHOOL OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570072/
• 关键词: HYPOTHALAMUS; PATCHCLAMP METHOD; OSMOSENSITIVITY; SLICE; OPIOID; パッチ・クランプ法; 単離した視床下部神経細胞; 視索上核; 第3脳室側壁部; 瞬時外液交換法; ナトリウム電流

1. To test osmosensitivity of hypothalamic neurons, the patch clamp method and the concentration clamp were used in the present study. Whole cell recordings were obtained from neurons in the regions of the anteroventral third ventricle (AV3V) which were isolated enzymatically and mechanically from 7 to 15-day-old rats. We analyzed chance of voltage-dependent sodium current with osmotic challenge. The results demonstrated the view that osmotic stimulation acts on neurons located in the AV3V region by modifying their membrane permeability for sodium ions with a slow kinetics.2. To clarify opioid-response of supraoptic neurons in the hypothalamus, slice preparation and extracellular recordings were used. The results suggest that opioids acting preferentially at kappa-receptors inhibit both vasopressin and oxytocin neurons while delta- and mu-receptor agonists inhibit primarily oxytocin neurons.

1. 采用膜片钳法和浓度钳法检测下丘脑神经元的渗透敏感性。对7 ~ 15日龄大鼠前腹侧第三脑室（AV3V）区域的神经元进行酶和机械分离，获得全细胞记录。我们分析了电压依赖性钠电流与渗透挑战的机会。研究结果表明，渗透刺激作用于AV3V区神经元是通过改变其对钠离子的膜透性来实现的。为了阐明下丘脑视上神经元的阿片反应，我们采用了切片制备和细胞外记录的方法。结果表明，阿片类药物优先作用于kappa受体，抑制抗利尿激素和催产素神经元，而delta和mu受体激动剂主要抑制催产素神经元。

项目成果

Inenaga K, Imura H, Yanaihara H, Yamashita H: "Kappa-selective opioid receptor agonists leumorphin and dynorphin inhibit supraoptic neurons in rat hypothalamic slice preparations" J of Neuroendocrinology.

Inenaga K、Imura H、Yanaihara H、Yamashita H：“Kappa 选择性阿片受体激动剂亮吗啡和强啡肽抑制大鼠下丘脑切片制剂中的视上神经元”《神经内分泌学杂志》。

Inenaga,K.,Akaike,N.and Yamashita,H.: "Osmotic influence on sodium current in isolated AV3V neurons" 産業医科大学雑誌. 11. 117-118 (1989)

Inenaga, K.、Akaike, N. 和 Yamashita, H.：“渗透对分离 AV3V 神经元中钠电流的影响”《职业与医学科学大学杂志》11. 117-118 (1989)。

Inenaga,K.,Imura,H.Yanaihara,H.and Yamashita,H.: "Kappaーselective opioid receptor agonists leumorphin and dynorphin inhibit suprnoptic neurons in rat hypothalamic slice preparations" J.Neuroendocrinology. (1990)

Inenaga, K.、Imura, H. Yanaihara, H. 和 Yamashita, H.：“κ 选择性阿片受体激动剂亮吗啡和强啡肽抑制大鼠下丘脑切片制剂中的视上切片神经元”J.Neuroendocrinology (1990)。

Inenaga,K.,Akaike,N.and Yamashita,H.: "Osmotic influence on sodium current in isolated AV3V neurous" 産業医科大学雑誌. 11. 117-118 (1989)

Inenaga, K.、Akaike, N. 和 Yamashita, H.：“渗透对孤立 AV3V 神经元中钠电流的影响”《职业与医学科学大学杂志》11. 117-118 (1989)。

Yamashita,H.Inenaga,K.and Dyball,REJ: "Chemical control of neurosevretory neurons in the SON" Recent Progress in Posterior Pitaitary Hormones. 3-10 (1988)

Yamashita，H.Inenaga，K. 和 Dyball，REJ：“SON 中神经锁神经元的化学控制”垂体后叶激素的最新进展。