ELECTROPHYSIOLOGICAL PROPERTIES AND OSMOSENSITIVITIES OF ISOLATED HYPOTHALAMIC NEURONS.

1.本研究应用膜片钳和浓度钳技术，观察了下丘脑神经元对多巴胺的敏感性。全细胞记录获得的神经元的前腹侧第三脑室（AV3V），其中酶和机械分离7至15日龄大鼠的区域。我们分析了渗透压激发下电压依赖性钠电流的机会。实验结果表明，渗透压刺激对AV3V区神经元的作用是通过改变其膜对钠离子的通透性而实现的，其动力学过程缓慢.为了阐明下丘脑视上神经元对阿片的反应，采用了切片制备和细胞外记录。结果表明，阿片类药物优先作用于κ受体抑制加压素和催产素神经元，而δ和μ受体激动剂主要抑制催产素神经元。

1. To test osmosensitivity of hypothalamic neurons, the patch clamp method and the concentration clamp were used in the present study. Whole cell recordings were obtained from neurons in the regions of the anteroventral third ventricle (AV3V) which were isolated enzymatically and mechanically from 7 to 15-day-old rats. We analyzed chance of voltage-dependent sodium current with osmotic challenge. The results demonstrated the view that osmotic stimulation acts on neurons located in the AV3V region by modifying their membrane permeability for sodium ions with a slow kinetics.2. To clarify opioid-response of supraoptic neurons in the hypothalamus, slice preparation and extracellular recordings were used. The results suggest that opioids acting preferentially at kappa-receptors inhibit both vasopressin and oxytocin neurons while delta- and mu-receptor agonists inhibit primarily oxytocin neurons.