Molecular biologic and histopathologic study on the expression status of Ca^<2+> binding protein genes in oral carcinoma

点击翻译按钮获取中文摘要

We examined many oral carcinoma, precancerous lesions, and normal tissues for the expression status of Ca^<2+> binding protein genes using our in-house cDNA microarray consisting 2201 cDNA clones Affymetrix GeneChip^<TM> consisting 48000 probes. The data of microarray analyses were confirmed by quantitative real time PCR.Twenty seven genes showed differential over-expression and 83 genes showed differential down expression in oral carcinoma, compared with those in normal tissue. Out of Ca^<2+> pump genes of endoplasmic reticulum ATP2a family genes were detected their suppressed expression. Expression of ATP2b family genes, which work as Ca^<2+> pump on the cell membrane, and calsequestrin-1 gene, which maintains the concentration of Ca^<2+> in the endplasmic reticulum, were also detected to be suppressed. The suppression of gene expression of S100 family genes, which play many roles, were also revealed.These results suggested that the carcinogenesis may resulting in slow switching of many systems in the carcinoma cells.