Molecular biologic and histopathologic study on the expression status of Ca^<2+> binding protein genes in oral carcinoma

口腔癌Ca^2结合蛋白基因表达状态的分子生物学和组织病理学研究

• 批准号: 16591820
• 负责人: OGAWARA Katsunori
• 金额: $ 2.24万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591820/
• 关键词: oral carcinoma; Ca^<2+> binding protein; microarray; gene expression profile; predictive carcinogenesis; Ca結合タンパク; マイクロアレイ解析; ATP2A遺伝子群; ATP2B遺伝子群; S100遺伝子群

We examined many oral carcinoma, precancerous lesions, and normal tissues for the expression status of Ca^<2+> binding protein genes using our in-house cDNA microarray consisting 2201 cDNA clones Affymetrix GeneChip^<TM> consisting 48000 probes. The data of microarray analyses were confirmed by quantitative real time PCR.Twenty seven genes showed differential over-expression and 83 genes showed differential down expression in oral carcinoma, compared with those in normal tissue. Out of Ca^<2+> pump genes of endoplasmic reticulum ATP2a family genes were detected their suppressed expression. Expression of ATP2b family genes, which work as Ca^<2+> pump on the cell membrane, and calsequestrin-1 gene, which maintains the concentration of Ca^<2+> in the endplasmic reticulum, were also detected to be suppressed. The suppression of gene expression of S100 family genes, which play many roles, were also revealed.These results suggested that the carcinogenesis may resulting in slow switching of many systems in the carcinoma cells.

我们使用由2201个cDNA克隆组成的cDNA微阵列（AffysseGeneChip），包括48000个探针，检测了许多口腔癌、癌前病变和正常组织中Ca ^2+结合蛋白基因的表达状态。<TM>实时荧光定量PCR结果显示，与正常组织相比，27个基因在口腔癌组织中呈差异性过表达，83个基因呈差异性下调表达。内质网Ca ^2+泵基因中ATP 2a家族基因表达被抑制。ATP 2b家族基因（作为细胞膜上的Ca ^2+泵）和钙螯合蛋白-1基因（维持内质网中Ca ^2+浓度）的表达也被检测到受到抑制。S100家族基因的表达也受到抑制，提示肿瘤的发生可能导致肿瘤细胞内多个系统的缓慢转换。

项目成果

Plasma membrane Ca^<2+> ATPase isoform 1 down-regulated in human oral cancer

质膜 Ca^<2 > ATPase 亚型 1 在人类口腔癌中下调

• 发表时间: 2006
• 期刊: Oncol Rep 15
• 作者: Saito K;Uzawa K;Endo Y;Kato Y;Nakashima D;Ogawara K;Shiba M;Bukawa H;Yokoe H;Tanzawa H
• 通讯作者: Tanzawa H

Sarcoendoplasmic reticulum Ca2+ ATPase type 2 downregulated in human oral squamous cell carcinoma

• DOI: 10.1002/ijc.20118
• 发表时间: 2004-06-10
• 期刊: INTERNATIONAL JOURNAL OF CANCER
• 影响因子: 6.4
• 作者: Endo, Y;Uzawa, K;Tanzawa, H
• 通讯作者: Tanzawa, H

Chromosome 1 open reading frame 10(Clorf10) gene is frequently down-regulated and inhibits cell proliferation in oral squamous cell carcinoma

口腔鳞状细胞癌中1号染色体开放阅读框10(Clorf10)基因频繁下调并抑制细胞增殖

• 发表时间: 2005
• 期刊: Int J Biochem Cell Biol. 37
• 作者: Imai FL;Uzawa K;Nimura Y;Moriya T;Imai MA;Shiiba M;Bukawa H;Yokoe H;Tanzawa H.
• 通讯作者: Tanzawa H.

Chromosome 1 open reading frame 10 (C1orf10) gene is frequently down-regulated and inhibits cell proliferation in oral squamous cell carcinoma

• DOI: 10.1016/j.biocel.2005.02.005
• 发表时间: 2005-08-01
• 期刊: INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
• 影响因子: 4
• 作者: Imai, FL;Uzawa, K;Tanzawa, H
• 通讯作者: Tanzawa, H

Chromosome 1 open reading frame 10(Clorf10) gene is frquently down-regulated and inhubits cell proliferation in oral squamous cell carcimoma

口腔鳞状细胞癌中 1 号染色体开放阅读框 10 (Clorf10) 基因经常下调并抑制细胞增殖

• 发表时间: 2005
• 期刊: Int J Biochem Cell Biol. 37
• 作者: Imai FL;Uzawa K;Nimura Y;Moriya T;Imai MA;Shiiba M;Bukawa H;Yokoe H;Tanzawa H
• 通讯作者: Tanzawa H