Elucidation of existence situation of amylose in starch granule

关于直链淀粉和支链淀粉在淀粉粒中的分子组成及其空间分布情况，目前尚无公认的理论。为了解决这个问题，我们尝试开发了这两种方法。首先，对淀粉颗粒进行球磨处理。也就是说，我们发现，在不加热和不加水的情况下，球磨处理很容易使淀粉颗粒糊化。这一事实表明，通过调整球磨条件可以很容易地控制糊化程度。因此，如果能找到良好的球磨条件，支链淀粉结晶区的部分溶解或熔融是可能的，从而在保持原有颗粒结构不变的情况下，使颗粒膨胀。膨化颗粒用与直链淀粉特异反应的特殊试剂处理，淀粉酶或该酶处理的颗粒与支链淀粉特异反应。这些处理可以修复直链淀粉在颗粒中的位置或显示直链淀粉。在我们的螺柱…中实践证明，球磨处理非常有利于解决这一问题。并通过超高压处理，成功地引入了脂肪酸作为特定试剂的模型。我们证明了通过改变淀粉的品种，这种脂肪酸被特异性地诱导为直链淀粉。这一事实表明直链淀粉是从支链淀粉中分离出来的。第二，支链淀粉分子从外到外的顺序水解。这将通过固定化淀粉酶来实现，该酶具有一定的限制性酶作用区。这个反应也会揭示直链淀粉，我们可以通过改变区域来控制反应。在这种情况下，应先用球磨机将颗粒糊化，然后再进行酶处理。我们成功地在磁性载体上制备了固定化普鲁兰酶，这使得酶很容易从反应混合物中的淀粉颗粒中分离出来。在下一阶段，我们尝试了支链淀粉在溶液中的顺序水解，并取得了成功。根据这些结果，我们成功地开发了一些用于本研究的方法，但我们无法得到这个问题的最终溶解。较少

There is no approval theory concerning to existence situation of molecular components < space distribution of amylose and amylopectin > in starch granule. To dissove this problem, we tried to develope the two methods. The first, ball-mill treatment of starch granule. That is, we found that starch granule is easily gelatinized by ball-mill traetment without heat and water. This fact suggests that we can easily control gelatization degree by regulation of ball-mill condition. Hence, it would be possible to be partial dissolution or melting of crystal region of amylopectin if we could find good ball-mill condition, and as the result, the granule would be expanded with retaining its original construction of granule. The expanded granule would be treated with special reagent which specificially react to amylose, and the granule treated with amylase or with the enzyme specificially react to amylopectin. These treatment could fix amylose situation in the granule or reveal amylose. In our stud … More y, it is proved that ball-mill treatment is very favorable to dissolve this problem. And we succeeded in introduction of fatty acid as the model of specific reagent by ultra high pressure treatment. We proved that the fatty acid is specificially induced to amylose by changing starch vareity. This fact suggest amylose being isolated from amylopectin. The second, sequential hydrolysis of amylopectin molecule from outside. This would be performed by immobilized amylase which has some extent of restricted enzyme action region. This reation would also reveal amylose and we could control the reaction by changing the region. In this case, we should gelatinized the granule by ball-mill before enzyme treatment. We succeeded in making immobilized pullulanase on magnetic support, and this makes possible quite easy separation from the enzyme from starch granule in reation mixture. In next stage, we tried to sequential hydrolysis of amylopectin in solution, and we succeeded.From these results, we succeeded in development of some methods for this study, however, we could not obtain the final dissolution of this problem. Less