Establishment of highly gene transcriptional regulation mediated by artificial microRNA in soybean
Establishment of highly gene transcriptional regulation mediated by artificial microRNA in soybean
批准号:
23580002
负责人:
YAMADA Tetsuya
金额:
$3.58万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2013
中文摘要
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英文摘要
Artificial mircoRNA (amiRNA) was designed using the precursor sequence of the endogenous soybean miRNA to suppress the expression of target gene efficiently in soybean plants. The constructed amiRNA precursor contains a complementary sequence to the transcript of target gene. Transgenic soybean plants expressing the amiRNA were generated to suppress the gene expression of seed storage protein. The expression of these genes and accumulation of storage protein were significantly decreased in seeds of transgenic soybean. In addition, the function of glycosyltransferase gene for DDMP saponin, which is known to be one of functional chemical compounds in soybean seeds, was characterized by the amiRNA expression system. I demonstrated that the knock-down system established in this study was useful for the functional analysis of genes in soybean plant.
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ダイズDDMPサポニンのラムノース転移に関わる遺伝子の同定
大豆DDMP皂苷鼠李糖转移相关基因的鉴定
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
[澤田純明, 平田和明, 香月遼・川崎翔太・山下祐佳・塚本知玄・喜多村啓介・阿部純・山田哲也]
通讯作者:
香月遼・川崎翔太・山下祐佳・塚本知玄・喜多村啓介・阿部純・山田哲也
野生ダイズにおけるDDMPサポニンのラムノース転移に関わる変異体の分子遺伝学的解析
野生大豆DDMP皂苷鼠李糖转移突变体的分子遗传学分析
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Than Myint Htun, Takashige Ishii, Ryo Ishikawa, 香月遼・川崎翔太・山下祐佳・塚本知玄・喜多村啓介・阿部純・山田哲也]
通讯作者:
香月遼・川崎翔太・山下祐佳・塚本知玄・喜多村啓介・阿部純・山田哲也
人口マイクロRNAを介したダイズ貯蔵タンパク質の質的改変
通过人工 microRNA 对大豆储存蛋白进行定性修饰
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
[松浦梨恵, 鶴切真由美, 花神彩香, 岸本利彦, イン・ベイウェン, 四方哲也, 森芳弘・安江一穂・丸山伸之・阿部純・山田哲也]
通讯作者:
森芳弘・安江一穂・丸山伸之・阿部純・山田哲也
ダイズDDMPサポニンのラムノース転移に関わる候補遺伝子の解析
大豆DDMP皂苷鼠李糖转移候选基因分析
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[安達登, 篠田謙一, 梅津和夫, 安達登, 安達 登, 香月遼・川崎翔太・山下祐佳・塚本知玄・喜多村啓介・阿部純・山田哲也]
通讯作者:
香月遼・川崎翔太・山下祐佳・塚本知玄・喜多村啓介・阿部純・山田哲也
DOI:
10.1270/jsbbs.61.480
发表时间:
2012-01
期刊:
Breeding science
影响因子:
2.4
作者:
[Yamada T, Takagi K, Ishimoto M]
通讯作者:
Ishimoto M
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