Study on depression of light-induced color reversion by treatment of mechanical pulps with extracellular crude enzymes from fungi.

本研究旨在通过真菌胞外粗酶处理抑制机械浆(MP)的光诱导返色。1.筛选具有抑制返色能力的真菌。建立了用两种还原指示剂筛选具有抑制返色能力的真菌的方法。从自然界分离到4株具有抗抑郁能力的真菌(编号：801、1304、501和1701)。真菌1701对Mp返原率的抑制率为56%。2.通过真菌原生质体融合形成具有抑制能力的融合子，由黄孢原毛平革菌和茄病镰刀菌原生质体融合形成2个融合子(1和2)。融合子1和融合子2分别能抑制181%的MP颜色还原，抑制能力一直保持到3次传代，但经过4次传代后，抑制能力大大降低。3.抑制MP颜色还原的酶的分离。1~4分对抑郁的预测能力分别为60、43、35、54%。4.以苄基乙酰香草酮为原料，经8步反应合成了具有彩色reversion.Guiacylglyceryl-alpha-keto-beta-0-4-coniferylaldehyde醚官能团的模型化合物。以苄基乙酰香草酮为原料，经4步反应制得关键化合物，总收率45%。最终化合物的合成正在进行中。

The aim of the study was depression of light-induced color reversion of mechanical pulps (MP) by treatment with extracellular crude enzymes from fungi.1.Screening of fungi having ability for the depression of the color reversion.Method of screening of fungi having ability for the depression of the color reversion with two reductive indicators was developed. Four fungi (No.801,1304,501 and 1701) having the ability for the depression were isolated from natural world. Fungus 1701 depressed 56% of the color reversion of MP.2.Formation of fusants having ability for the depression by protoplast fusion of fungi.Two fusants (1 and 2) were formed by protoplast fusion of Phanerochaete chrysosporium and Fusarium solani. One hundred and eighty one percent of the color reversion of MP could be depressed by fusants 1 and 2 respectively.The ability for the depression has been maintaied until three subcultures, however, the ability was largely decreased after four subcultures.3.Fractionation of enzymes responsible for depression of the color reversion of MP.Extracellular crude enzymes prepared from culture medium of Trametes serialis were separated into four fractions (Fr.1-Fr.4) by ion-exchange chromatography. Each ability of Fr.1 to 4 for the depression was 60,43,35,54%, respectively. Further fractionation of Fr.1 and 4 are being conducted.4.Synthesis of medel compound having functional groups for the color reversion.Guiacylglyceryl-alpha-keto-beta-0-4-coniferylaldehyde ether was tried to synthesize from benzyl acetovanillone through 8 steps. The key compound for the synthesis was prepared from benzy1 acetovanillone through 4 steps in the total yield of 45%. The synthesis of the final compound is being conducted.