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Bioremediation of dioxins with dioxins degradable fungi and its fusants formed by protoplast fusion of the fungi

二恶英降解真菌及其原生质体融合体对二恶英的生物修复

基本信息

批准号：
11660166
负责人：
TACHIBANA Sanro
金额：
$ 2.11万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660166/
关键词：
Dioxins Screening Bioremediation Protoplast fusion Incinerated ashes Contaminated soils Purification of Environment Dioxins degradable fungi 土壌

项目摘要

Biodegradation of dioxins in soils and incinerated ashes by bioremediation was conducted by use of dioxins degradable fungi and its fusants formed by protoplast fusion of the fungi. And, formation of fusants having higher ability for degradation of dioxins formed by the fusion of the fungi were also conducted.1. Each dioxin (2, 7-DCDD, 2, 4, 8-TCDF and 2, 3, 7, 8-TCDD) in soils could be degraded by bioremediation with dioxins degradable fungi. Each dioxin was degraded about 70%, 65% and 60%, respectively, for 30 days.2. About 90% of dioxins in paddy filed soils was degraded by bioremediation with dioxins degradable fungi for 30 days. The toxicity of the dioxins in the solis was alos removed by the bioremediation. And furthermore, the degree of degradation of the dioxins was increased by addition of a surfactant during the bioremediation.3. About 50% of dioxins in nicinerated ashes was degraded by bioremediation with dioxins degradable fungi for 60 days. It was found that the degree of the dioxins in the ashes was considerably increased by repetition of the bioremediation.4. Dioxins in paddy filed soils could be degraded by bioremediation with fusants formed by prptoplast fusion of the fungi. The degree of degradation of the dioxins was a little higher than that by the bioremediation with the fungi. In addition, the ability of fusants for degradation of dioxins decreased in the repeated subcultures.

利用二恶英降解菌及其原生质体融合融合子对土壤和焚烧灰中二恶英进行生物修复降解。并且，还进行了融合子的形成，融合子对真菌融合形成的二恶英具有更高的降解能力.土壤中的各种二恶英（2，7-DCDD、2，4，8-TCDF和2，3，7，8-TCDD）均可通过二恶英降解菌的生物修复作用降解。30 d后，各二恶英的降解率分别为70%、65%和60%.利用二恶英降解菌对稻田土壤进行生物修复30 d后，二恶英降解率可达90%左右。生物修复还可以去除土壤中二恶英的毒性。此外，在生物修复过程中加入表面活性剂，提高了二恶英的降解程度.用二恶英降解菌对焚烧灰进行生物修复60 d，二恶英降解率约为50%。研究发现，生物修复的重复使用，大大增加了灰渣中二恶英的含量.利用真菌原生质体融合形成的融合子进行生物修复，可降解稻田土壤中的二恶英。二恶英的降解程度略高于真菌的生物修复。此外，融合子降解二恶英的能力下降，在重复的继代培养。