Study on utilization of wood extractives from Kyaraboku (Taxus cuspidata var.nana) belonging to trees of genus taxus

红豆杉属木材提取物的利用研究

• 批准号: 07556044
• 负责人: TACHIBANA Sanro
• 金额: $ 3.07万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07556044/
• 关键词: Tissue cultures; Elicitor; Taxus cuspidata var.nana; Taxol; Anti-biotic activity; Callus cultures; Chitoheptaose; Cell suspension cultures; 固定化細胞; キトオリゴ糖; 生合成前駆体; 微生物変換; ジベレリン

Utilization of wood extractives from Kyaraboku were examined.1.Taxol, a strong anti-tumor agent, in T.cuspidata was produced by callus cultures of Kyaraboku. Amount of production of taxol was increased 5.1 times larger than that of the control (no addition) when chito-oligosaccharides as a elicitor for ehhancement of taxol production were added to the calli. Chitoheptaose was found to be the best elicitor in the chito-oligosaccharides.2.Taxol was produced by cell suspension cultures of Kyaraboku. Taxol production increased 6.3 times larger than that of the control when chitoheptaose and jasmonic acid were added to the suspension cultures.3.Taxol was released into the medium during incubation when cultured cells were incorporated with sodium alginate and incubated. The maximum amount of taxol released into the medium was 0.6 mg/L.4.Taxinine in Kyaraboku and its derivatives have anti-biotic activities against Cochiliobolus miyabeanus, Altenaria kikuchiana, Colletotrichum fragariae, Botryisis cinerea, and Corynespora cassiicola.5.A trial of preparation of taxol mimic by condensation of the BaccatinIII like compound derived from taxinine and phenylisosserin was conducted. Phenylisoserin, the side chain of taxol, was stereoselectively synthesized from benzaldehyde in a total yield of 0.9%. However, preparation of the BaccationIII like compound from taxinine are now being conducted.

本文研究了东北红豆杉木材提取物的开发利用。1.东北红豆杉愈伤组织培养产生紫杉醇，紫杉醇是一种抗肿瘤活性物质。在愈伤组织中加入壳寡糖诱导紫杉醇的合成，紫杉醇的合成量比对照增加了5.1倍。结果表明，壳七糖是壳寡糖中最好的诱导子。加入壳七糖和茉莉酸后，紫杉醇的产量比对照提高了6.3倍。3.海藻酸钠与细胞共孵育时，紫杉醇释放到培养基中。紫杉醇在培养基中的最大释放量为0.6mg/L。4.京白中的紫杉碱及其衍生物对宫城拟盘孢霉、菊地链格孢霉、草莓炭疽菌、灰葡萄孢和烟草棒孢霉具有一定的抗菌活性。以苯甲醛为原料，立体选择性地合成了紫杉醇侧链苯基异丝氨酸，总收率为0.9%。然而，现在正在进行从紫杉碱制备BaccationIII样化合物。

项目成果

上野 淳一郎: "イチイ科樹木の抽出成分(VI)イチイ葉の抽出成分について" 第46回日本木材学会大会研究発表要旨集. 407-407 (1996)

Junichiro Ueno：“红豆杉树的提取成分（六）关于红豆杉叶的提取成分”第46届日本木材学会会议摘要407-407（1996）。

K.Kurose: "Extractives of Taxus cuspidata Sieb.et.Zucc.Leaves and chemoeaxonomy of Trees of Genus Taxus" Bull.Ehime University Forest. 第33号. 1-16 (1995)

K.Kurose：“红豆杉提取物 Sieb.et.Zucc.Leaves 和红豆杉属树木的化学分类学”Bull.Ehime University Forest No. 33. 1-16 (1995)

吉田 真弓: "イチイ科樹木の組織培養による抗ガン性物質,タキソ-ルの生産の試み" 第46回日本木材学会大会研究発表要旨集. 7-7 (1996)

Mayumi Yoshida：“尝试通过红豆杉树的组织培养来生产紫杉醇，一种抗癌物质”第 46 届日本木材学会会议上的研究报告摘要 7-7（1996 年）。

Katsuhiko Kurose et al.: "Extractives of Taxus cuspidata Sieb.et Zucc. Leaves and Chemotaxonomy of Trees of Genus Taxus." Bull.Ehime Univ.Forest. No.33. 1-16 (1995)

Katsuhiko Kurose 等人：“红豆杉 Sieb.et Zucc 的提取物。红豆杉属树木的叶子和化学分类学”。

村中俊夫: "キャラボク葉中の成分、及びその誘導体の抗菌性" 第47回日本木材学会大会研究発表要旨集. 591-591 (1997)

Toshio Muranaka：“Charaboku 叶及其衍生物中成分的抗菌特性”第 47 届日本木材学会会议上的研究报告摘要 591-591（1997 年）。