Research and development on purification methods for dioxins, environmental ha-manes and indnerated ashes by use of a minnbial preparations and an enzyme preparations prepared from a fungus capable of degradation of dioxins

利用一种能降解二恶英的真菌制备的微生物和酶制剂，研究了二恶英、环境激素和焚烧灰的净化方法，取得了以下结果：通过对微生物制剂的添加量、处理时间等方面的研究，建立了利用微生物制剂净化二恶英和环境激素污染土壤的方法。使用5%的制剂，84%的ddt污染土壤可以用该方法净化30天。用该方法对35 ~ 66%的二恶英（2,8- dcdd和2,4,8- tcdf）污染土壤进行了30天的净化。此外，还研究了在土壤中添加营养物促进真菌生长的方法。从真菌中提取粗酶，经胶囊化法制备酶制剂。利用该制剂开发了污染土壤的净化方法，同时也开发了污染水体的净化方法。滴滴涕和二恶英（TCB）污染土壤分别有27%和15%用该方法净化30天。使用该制剂3 d， DIM污染水体的净化率为35%，tcb污染水体的净化率为26%。此外，通过澄清稳定因子可以稳定制剂中的酶活性。当用该方法净化污染水时，所制备的制剂可重复使用至少两次。并对其降解性能进行了评价。研究了利用具有pH缓冲能力的土壤和促进真菌生长的表面活性剂的微生物制剂净化焚烧灰的方法。灰分中约40%的二恶英（PCDD/Fs）用该方法纯化30天。然而，酶制剂对灰分中PCDD/Fs的降解几乎不能以灰分碱度导致酶失活为前提。土壤中原本存在的微生物和与土壤微生物活性相关的脱氢酶活性对微生物制剂和酶制剂处理土壤没有影响。植物栽培对土壤处理的影响也未被认识到。少

The purification methods for dioxins, environmental hormones, and incinerated ash were tried to develop by use of microbial and enzyme preparations made from a fungus capable of degradation of dioxins, and the following results were obtained.1. The purification method of dioxin- and environmental hormone-contaminated soils by use of the microbial preparations was developed by investigating the added amount of the preparations and treatment time and so on. Eight four percent of the DDT-contaminated soil could be purified by the method for 30 days with 5% of the preparations. And, 35 to 66% of the dioxins (2,8-DCDD and 2,4,8-TCDF)-contaminated soils were purified by the method for 30 days with 2 to 5% of the preparations. Furthermore, the method for enhancement of growth of the fungus on the preparations in soil was also developed by addition of nutrients.2. Enzyme preparations were made from a crude enzyme from the fungus by capsulation. The purification methods of the contaminated soil … More s as well as those waters were developed by using the preparations. Twenty seven and 15% of DDT- and dioxin (TCB)-contaminated soils were purified by the method for 30 days, respectively. And, thirty five and 26% of DIM' and TCB-contaminated waters were also purified by using the preparations for 3 days, respectively. Furthermore, the enzyme activity in the preparations could be stabilized by clarification of the stabilizing factors. The preparations could be reused at least two times when the contaminated waters were purified by the method. The evaluation method for degradation capability of the preparations was also developed.3. The purification method for an incinerated ash was developed by using the microbial preparations with soil having pH buffer capacity and a surfactant enhancing growth of the fungus. About 40% of dioxins (PCDD/Fs) in the ash were purified by the method for 30 days. However, degradation of PCDD/Fs in the ash with the enzyme preparations could not be almost preceded by the inactivation of enzymes for the alkalinity of ash.4. There was no effect of microorganisms originally existed in the soil and dehydrogenase activity related to the activity of microorganisms in soil on the treatment of soil with the microbial and enzyme preparations. Effect of plant cultivation on the soil treatment was not also recognized. Less