Research and development on purification methods for dioxins, environmental ha-manes and indnerated ashes by use of a minnbial preparations and an enzyme preparations prepared from a fungus capable of degradation of dioxins

利用降解二恶英的真菌制剂和酶制剂净化二恶英、环境哈尼和焚烧灰方法的研究与开发

• 批准号: 18580166
• 负责人: TACHIBANA Sanro
• 金额: $ 2.39万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580166/
• 关键词: Purification; Dioxins; Environmental hormones; White-rot fungi; Microbial preparations; Enzyme preparations; Dioxin-contaminated water; ダイオキシン汚染土壌; バイオリアクター

The purification methods for dioxins, environmental hormones, and incinerated ash were tried to develop by use of microbial and enzyme preparations made from a fungus capable of degradation of dioxins, and the following results were obtained.1. The purification method of dioxin- and environmental hormone-contaminated soils by use of the microbial preparations was developed by investigating the added amount of the preparations and treatment time and so on. Eight four percent of the DDT-contaminated soil could be purified by the method for 30 days with 5% of the preparations. And, 35 to 66% of the dioxins (2,8-DCDD and 2,4,8-TCDF)-contaminated soils were purified by the method for 30 days with 2 to 5% of the preparations. Furthermore, the method for enhancement of growth of the fungus on the preparations in soil was also developed by addition of nutrients.2. Enzyme preparations were made from a crude enzyme from the fungus by capsulation. The purification methods of the contaminated soil … More s as well as those waters were developed by using the preparations. Twenty seven and 15% of DDT- and dioxin (TCB)-contaminated soils were purified by the method for 30 days, respectively. And, thirty five and 26% of DIM' and TCB-contaminated waters were also purified by using the preparations for 3 days, respectively. Furthermore, the enzyme activity in the preparations could be stabilized by clarification of the stabilizing factors. The preparations could be reused at least two times when the contaminated waters were purified by the method. The evaluation method for degradation capability of the preparations was also developed.3. The purification method for an incinerated ash was developed by using the microbial preparations with soil having pH buffer capacity and a surfactant enhancing growth of the fungus. About 40% of dioxins (PCDD/Fs) in the ash were purified by the method for 30 days. However, degradation of PCDD/Fs in the ash with the enzyme preparations could not be almost preceded by the inactivation of enzymes for the alkalinity of ash.4. There was no effect of microorganisms originally existed in the soil and dehydrogenase activity related to the activity of microorganisms in soil on the treatment of soil with the microbial and enzyme preparations. Effect of plant cultivation on the soil treatment was not also recognized. Less

本研究利用一种降解二恶英的真菌制备的微生物和酶制剂，对二恶英、环境激素和焚烧灰的净化方法进行了研究，取得了以下成果.通过考察微生物制剂的添加量、处理时间等因素，建立了微生物制剂对二恶英和环境污染土壤的净化方法，用5%的微生物制剂处理30 d，可净化84%的DDT污染土壤。并且，35 - 66%的二恶英（2，8-DCDD和2，4，8-TCDF）污染的土壤被净化30天的方法与2 - 5%的制剂。此外，还研究了通过添加营养物质促进该菌在土壤中生长的方法.酶制剂由来自真菌的粗酶通过胶囊化制备。污染土壤的净化方法 ...更多信息 s以及那些沃茨通过使用这些制剂来开发。分别对27%和15%的DDT和二恶英（TCB）污染土壤进行了30天的净化。并且，通过使用制剂3天，还分别净化了35%和26%的DIM和TCB污染的沃茨。此外，通过澄清稳定因素，可以稳定制剂中的酶活性。该方法净化污染沃茨时，制剂可重复使用至少两次。建立了制剂降解性能的评价方法.利用具有pH缓冲能力的土壤微生物制剂和促进真菌生长的表面活性剂开发了焚烧灰的净化方法。30 d的净化效果表明，煤灰中二恶英（PCDD/Fs）的去除率可达40%左右。但是，煤灰中PCDD/Fs的降解几乎不能在酶失活之前进行.土壤中原有的微生物和与土壤中微生物活性相关的脱氢酶活性对微生物和酶制剂处理土壤没有影响。植物栽培对土壤处理的影响也没有得到承认。少

项目成果

Trails for degradation of DDT-contaminated waste water by an ezyme Preparations from white-rot fungi

白腐真菌制剂降解DDT污染废水的试验

• 发表时间: 2007
• 作者: Satoshi;Somamoto;Keita;Fijii;Kazutaka;Itoh;Sanro;Tachibana
• 通讯作者: Tachibana

白色腐朽菌からの微生物製剤作製の試み

尝试从白腐真菌中制备微生物制剂

• 发表时间: 2006
• 期刊: 第51回リグニン討論会講演集
• 作者: Hiromitsu Kisanuki;Arisa Nakai;Akiko Nadamoto;Masako Wakino;木佐貫博光・中村由実・中井亜理沙・石田清;木佐貫博光・灘本明子・林佐智子・脇野雅子・中井亜理沙;木佐貫博光;木佐貫博光;佐々木 良輝
• 通讯作者: 佐々木 良輝

数種の担体を用いた微生物製剤作製の試み

尝试使用多种类型的载体生产微生物制剂

• 发表时间: 2007
• 作者: 塔村真一郎;宮本康太;石川敦子;井上明生;古山 健太
• 通讯作者: 古山 健太

ダイオキシン分解菌からの酵素を用いた酵素製剤作製の試み

尝试使用二恶英降解菌的酶生产酶制剂

• 发表时间: 2006
• 期刊: 第51回リグニン討論会講演集
• 作者: Hiromitsu Kisanuki;Arisa Nakai;Akiko Nadamoto;Masako Wakino;木佐貫博光・中村由実・中井亜理沙・石田清;木佐貫博光・灘本明子・林佐智子・脇野雅子・中井亜理沙;木佐貫博光;木佐貫博光;佐々木 良輝;塔村真一郎;藤井 啓太
• 通讯作者: 藤井 啓太

白色腐朽菌からの酵素製剤を用いた水中のDDT分解の試み

尝试使用白腐真菌酶制剂降解水中的DDT

• 发表时间: 2007
• 作者: 宮本康太;井上明生;塔村真一郎;杣本 聡
• 通讯作者: 杣本 聡