权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Degradation and purification of dioxins by bioremediation with fungi having ability for degradation of dioxins

利用具有二恶英降解能力的真菌进行生物修复对二恶英的降解和纯化

基本信息

批准号：
11694214
负责人：
TACHIBANA Sanro
金额：
$ 4.35万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

Screening of fungi having ability for degradation of dioxins from the nature was conducted in temperate and tropical zone, and selected several fungi. Degradation of dioxins in soils, incinerated ashes and water by bioremediation with the selected fungi was conducted. And, furthermore, formation of fusants having higher ability for degradation of dioxins was conducted by protoplast fusion between the two selected fungi.1. Dioxins (2,7-DCDD, 2,8-DCDD, 2,4,8-TCDF, 2,3,7,8-TCDD, 2,3,4,7,8-PCDF and OCDD) were degraded by the selected fungi from the nature in a liquid medium. The degree of degradation of each dioxin was about 95%, 85%, 70%, 60%, 95% and 40%, respectively for 30 days incubation.2. Biodegradation of dioxins (2,7-DCDD, 2,4,8-TCDF and 2,3,7,8-TCDD) in soils could be degraded by bioremediation with the selected fungi. Each dioxin was degraded about 70%, 60% and 50%, respectively for 30 days.3. About 70% of dioxins in the soils (contamination level: about 1300pg-TEQ/g) was degraded by bioremediation with the selected fungi for 30 days. About 70% of the toxicity of dioxins in the soils was also removed by the bioremediation. And furthermore, the degree of degradation of the dioxins was increased about 10% by addition of a surfactant when the bioremediation was conducted.4. About 65% of dioxins in incinerated ashes was degraded by bioremediation with the selected fungi for 60 days. It was found that the degree of degradation of dioxins in the ashes could be almost completely degraded by the repeated treatment of the bioremediation.5. Dioxins in aqueous environment could be degraded considerably by bioremediation with the selected fungi after adsorption of dioxins by activated carbons and mixing of non-polluted soils with the adsorbed carbons. It was found that dioxins in water were almost completely adsorbed on to activated carbons.

从温带和热带地区自然界中筛选具有降解二恶英能力的真菌，筛选出几株真菌。利用所选真菌对土壤、焚烧灰和水中二恶英进行生物修复降解。此外，通过原生质体融合，筛选出了降解二恶英能力较强的融合子.在液体培养基中，利用自然界中筛选出的真菌对二恶英（2，7-DCDD，2，8-DCDD，2，4，8-TCDF，2，3，7，8-TCDD，2，3，4，7，8-PCDF和OCDD）进行降解。培养30 d，各二恶英的降解率分别为95%、85%、70%、60%、95%和40%.利用所选真菌对土壤中二恶英（2，7-DCDD、2，4，8-TCDF和2，3，7，8-TCDD）进行生物修复，可有效降解土壤中的二恶英。30 d后，各二恶英的降解率分别为70%、60%和50%.土壤中二恶英的污染水平约为1300 pg-TEQ/g，经30 d的生物修复，降解率约为70%。土壤中二恶英的毒性也被生物修复去除了约70%。此外，在生物修复过程中，添加表面活性剂可使二恶英的降解率提高约10%.经60 d的生物修复试验，焚烧灰中二恶英的降解率约为65%。研究发现，通过生物修复的重复处理，可以使灰渣中二恶英的降解程度几乎完全降解.活性炭吸附二恶英后，与非污染土壤混合，利用筛选出的真菌进行生物修复，可显著降解水体中的二恶英。结果发现，水中的二恶英几乎完全吸附在活性炭上。