乳幼児腫瘍の自然退縮機構に関する細胞病理学及びアポトーシス関連遺伝子の研究

婴儿肿瘤自然消退机制的细胞病理学和凋亡相关基因研究

• 批准号: 06670197
• 负责人: 三杉 和章
• 金额: $ 1.02万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670197/
• 关键词: 乳幼児腫瘍; 神経芽細胞腫; マス・スクリーニング; TrkA; LNGFR; アポトーシス

1.研究内容の要約マス・スクリーニングで発見される神経芽腫のLNGFR(低親和性神経成長因子受容体)、tissue transglutaminase(TG)の遺伝子発現を検討した結果、スクリーニング例のうち少なからずのものがLNGFRを介したアポトーシスによる退縮機構が関与している可能性が示唆さた。2.研究目的プログラムされた細胞死の概念は腫瘍細胞の増殖様式を考えるうえで、細胞分裂と並んで不可欠のものとなりつつある。本研究では、ヒト腫瘍の自然退縮例をモデルとして、実際の腫瘍の増殖、退縮におけるアポトーシスの関与を分子レベルで明らかにすることを目的とした。3.研究方法とその結果小児の神経系腫瘍である神経芽腫では、生後6カ月の乳児の尿中カテコールアミンの増加を指標とするマス・スクリーニングが行われているが、スクリーニング例ではしばしば腫瘍の自然退縮が観察される。スクリーニング例43例、非スクリーニング例14例、LNGFR、TGの遺伝子発現を比較検討した結果、TGの発現亢進がスクリーニング例18例と化学療法後の非スクリーニング例1例で見られた。LNGFRの高発現はスクリーニング例の13例で見られたが、このうちTG高発現例は約85%の11例を占め、我々が実際に自然退縮を観察した1例はこれに含まれた。4.考察神経芽腫スクリーニング例に見られる自然退縮では、FAS抗原と相同性を持つLNGFRを介するアポトーシスが起こっているものと考えられる。今後、LNGFRの発現レベルを指標に、手術を施さずに自然退縮を持つことができる可能性が示唆されると同時に、進行例におけるアポトーシス誘導療法の研究にも有用な結果と思われる。

1. The content of the study is very important. Please see the LNGFR (low and sexual growth factor acceptor), the tissue transglutaminase (TG) growth factor, the results of the study, and the results of the study. The results show that there is a significant difference between the results of the study and the results of the study. The results show that there is a significant difference between the results of the study and the results of the study. The results show that there is a significant difference between the results of the study and the results of the study. The results show that there is a significant difference between the results of the study and the results of the study. The results show that there is a significant difference between the results of the study and the results of the study. The results show that there is a significant difference between the results of the study and the results of the study. The results show that there is a possibility that you will not be able to obtain information about the possibility that you may be involved in the treatment of LNGFR. two。 The purpose of this study is to study the concept of cell death, cell death, cell division and cell division. In this study, examples of natural withdrawal are reported in this study. The results show that there is no significant difference between the two groups. 3. The results of the study were as follows: the results of this study were as follows: the results of this study were as follows: the results of the study showed that the baby was born 6 months after birth, and the urine of the breast milk at 6 months after birth. There were 43 cases of chronic hepatitis, 14 cases of non-infectious diseases, 18 cases of abnormal symptoms in LNGFR and TG, and 18 cases of hyperactivity in TG. After chemical method, there were 1 case of abnormal symptoms. There are 13 cases of abnormal symptoms in LNGFR, 11 cases in 11 cases of TG, and 1 case in the world. 4. In the study of the sprouts and buds of the gods, the examples show that the FAS antigens are naturally degenerated, and the FAS antigens are homologous. The LNGFR antigens are similar to each other. From now on, the LNGFR will show that it is possible to use the guidance method for the study of the useful results of the study on the possibility of abetting the use of the guidance method for the study of the disease.

项目成果

Miyagi,Y.,Misugi,K.et al: "Studies for genes expressed in neuroblastomas detected by mass screening" International J Surg.Pathol.2. 446- (1994)

Miyagi,Y.,Misugi,K.等人：“通过大规模筛选检测到的神经母细胞瘤中表达的基因的研究”International J Surg.Pathol.2。

Miyagi,Y.,Misugi,K.et al: "Studies for genes expressed in neuroblastomas detected by mass screening" Proceedings,3rd Int.Symp.Neuroblastoma Screening.148-151 (1994)

Miyagi,Y.,Misugi,K.等人：“通过大规模筛选检测到的神经母细胞瘤中表达的基因的研究”Proceedings，第 3 届 Int.Symp.Neuroblastoma Screening.148-151 (1994)