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Influence of the chemical form of selenium in the diet on the tipid metabolism.

饮食中硒的化学形态对微量代谢的影响。

基本信息

批准号：
06670396
负责人：
KOYAMA Hiroshi
金额：
$ 1.41万
依托单位：
Tohoku University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670396/
关键词：
Selenium Lipid Metabolism Dietary Research HDL-Cholesterol Apo A-I Apo A-II HPLC-ICP-MS

项目摘要

Selenium is an essential trace element and the association with lipid metabolism has been suggested. Blood level of selenium has been used as an indicator of nutritional status of selenium. However, the level is dependent of chemical forms of selenium in the diet and plasma selenium can be divided into several groups including some selenoproteins and non-specific selenium-containing proteins.In the present study, 1) we have estimated selenium intake by having foods classified into several food groups, 2) the distribution of selenium in human plasma has been investigated by an HPLC-ICP mass spectrometry method, and then 3) the effects of the forms of selenium in the diet and the distribution of selenium in plasma on the lipid concentrations in blood serum is now evaluated.1) We have developed a new food intake frequency questionnaire and the results obtained from 50 women show that the questionnaire can detect 70.4% of daily selenium intake and among them 46.4% of selenium is from fishes, 45.5% is from animals (containing selenocysteine), and 8.0% is from cereals (mainly selenomethionine).2) The chromatographic pattern of selenium in human plasma was investigated by an HPLC equipped with a multi-mode column (Asahipak GS-520 HQ) coupled directly to an ICP-MS.Four peaks of selenium were detected in the chromatogram. The first peak represents selenium contained in the high molecular weight fraction such as lipoproteins, and the second peak is thought to be selenoprotein-P.The retention time of the third peak was in accordance with that of albumin. The results show that the method can divide plasma selenium into three components.3) is now continued.

硒是人体必需的微量元素，与脂质代谢有关。血硒水平已被用作硒营养状况的指标。然而，血浆硒的水平取决于饮食中硒的化学形式，血浆硒可分为几类，包括一些硒蛋白和非特异性含硒蛋白。在本研究中，1)我们通过将食物分为几个食物组来估计硒的摄入量，2)用HPLC-ICP质谱法研究了硒在人血浆中的分布，然后3)评估了硒在饮食中的形式和硒在血浆中的分布对血清脂质浓度的影响。1)我们编制了新的食物摄入频率问卷，对50名妇女的调查结果表明，该问卷能检测出每日硒摄入量的70.4%，其中鱼类占46.4%，动物占45.5%（含硒半胱氨酸），谷物占8.0%（含硒代蛋氨酸）。2)采用多模式色谱柱（Asahipak GS-520 HQ）与ICP-MS直接耦合的高效液相色谱法研究了人血浆中硒的色谱图谱。在色谱中检测到4个硒峰。第一个峰代表高分子量部分如脂蛋白中所含的硒，第二个峰被认为是硒蛋白- p。第三峰的保留时间与白蛋白的保留时间一致。结果表明，该方法可将血浆硒分解为三个组分。3)现在继续。