Influence of the chemical form of selenium in the diet on the tipid metabolism.

硒是人体必需的微量元素，与脂质代谢密切相关。血硒水平已被用作硒营养状况的指标。本研究通过将食物分为不同的食物组来估算硒的摄入量，采用高效液相色谱-电感耦合等离子体质谱法（HPLC-ICP-MS）研究了硒在人体血浆中的分布，3）探讨了硒在膳食中的存在形式及血浆中硒的分布对血脂水平的影响：1）设计了一种新的食物摄入频率调查表，对50名妇女的调查结果表明，该调查表可检出每日硒摄入量的70.4%，其中46.4%的硒来自鱼类，2）采用Asahipak GS-520 HQ多模式色谱柱与电感耦合等离子体质谱联用技术，研究了人血浆中硒的色谱特征。第一个峰代表高分子量组分如脂蛋白中所含的硒，第二个峰被认为是硒蛋白-P。第三个峰的保留时间与白蛋白的保留时间一致。结果表明，该方法可将血浆硒分为三个组分。

Selenium is an essential trace element and the association with lipid metabolism has been suggested. Blood level of selenium has been used as an indicator of nutritional status of selenium. However, the level is dependent of chemical forms of selenium in the diet and plasma selenium can be divided into several groups including some selenoproteins and non-specific selenium-containing proteins.In the present study, 1) we have estimated selenium intake by having foods classified into several food groups, 2) the distribution of selenium in human plasma has been investigated by an HPLC-ICP mass spectrometry method, and then 3) the effects of the forms of selenium in the diet and the distribution of selenium in plasma on the lipid concentrations in blood serum is now evaluated.1) We have developed a new food intake frequency questionnaire and the results obtained from 50 women show that the questionnaire can detect 70.4% of daily selenium intake and among them 46.4% of selenium is from fishes, 45.5% is from animals (containing selenocysteine), and 8.0% is from cereals (mainly selenomethionine).2) The chromatographic pattern of selenium in human plasma was investigated by an HPLC equipped with a multi-mode column (Asahipak GS-520 HQ) coupled directly to an ICP-MS.Four peaks of selenium were detected in the chromatogram. The first peak represents selenium contained in the high molecular weight fraction such as lipoproteins, and the second peak is thought to be selenoprotein-P.The retention time of the third peak was in accordance with that of albumin. The results show that the method can divide plasma selenium into three components.3) is now continued.