Nutritional epidemiology of selenium by chemical forms

分析三种含硒蛋白（白蛋白、谷胱甘肽过氧化物酶（GPx）和硒蛋白P）中哪一种与脑卒中的发生有关。我们进行了一项基于人群的病例对照研究，纳入了30例首次卒中病例，这些病例在卒中前于1992年至1994年进行了健康检查和血液采样，并与30例对照组进行了性别、年龄、总胆固醇和血液采样年份的匹配。血清中的总硒用荧光法测定。含硒蛋白质用两个串联的HPLC柱分离，硒用ICP-MS检测。中风病例的血清硒浓度倾向于低于对照组（105.2 vs 116.5 μg/l;p=0.054）。虽然GPx和白蛋白中的硒含量没有显示出任何显著差异，但脑卒中患者的硒蛋白P显著低于对照组（54.5 vs 63.0 μg/l;p=0.006）。多因素Logistic回归分析显示，硒蛋白P可降低脑卒中的危险性（OR =0.28，95%可信区间：0.10-0.8），提示硒蛋白P可能在脑卒中的发生发展中起重要作用。

To analyze which of the three selenium-containing proteins (albumin, glutathione peroxidase(GPx) and selenoprotein P) was responsible for the development of stroke. We conducted a population-based case-control study of 30 first-ever stroke cases who had undergone health examinations and blood sampling in 1992 to 1994 before the stroke, and 30 controls matched for their sex, age, total cholesterol, and the year of blood sampling. Total selenium in the serum was measured fluorometrically. Selenium-containing proteins were separated using two HPLC columns in tandem, and selenium was detected by ICP-MS.The serum concentration-of selenium tended to be lower in the stroke cases than in the controls (105.2 vs 116.5 μg/l;p=0.054). Although the selenium contents in GPx and albumin did not show any significant difference, selenoprotein P was significantly lower in the stroke cases than in the controls (54.5 vs 63.0 μg/l;p=0.006). A multiple logistic analysis showed that selenoprotein P can reduce the risk of stroke (Odds Ratio=0.28;95% Confidence Interval:0.10-0.8).The results of this study show that Selenoprotein P may play an important role in development of stroke.