Nutritional epidemiology of selenium by chemical forms

硒的化学形态营养流行病学

• 批准号: 13557032
• 负责人: KOYAMA Hiroshi
• 金额: $ 8.96万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13557032/
• 关键词: selenium; speciation analysis; glutathione peroxidase; selenoprotein P; albumin; food frequency questionare; グルタチオンペルオキシターゼ; アルプミン; 簡易食品摂取頻度調査票; 簡易食事接種頻度調査票; セレン化学形別分析; KKマウス

To analyze which of the three selenium-containing proteins (albumin, glutathione peroxidase(GPx) and selenoprotein P) was responsible for the development of stroke. We conducted a population-based case-control study of 30 first-ever stroke cases who had undergone health examinations and blood sampling in 1992 to 1994 before the stroke, and 30 controls matched for their sex, age, total cholesterol, and the year of blood sampling. Total selenium in the serum was measured fluorometrically. Selenium-containing proteins were separated using two HPLC columns in tandem, and selenium was detected by ICP-MS.The serum concentration-of selenium tended to be lower in the stroke cases than in the controls (105.2 vs 116.5 μg/l;p=0.054). Although the selenium contents in GPx and albumin did not show any significant difference, selenoprotein P was significantly lower in the stroke cases than in the controls (54.5 vs 63.0 μg/l;p=0.006). A multiple logistic analysis showed that selenoprotein P can reduce the risk of stroke (Odds Ratio=0.28;95% Confidence Interval:0.10-0.8).The results of this study show that Selenoprotein P may play an important role in development of stroke.

分析三种含硒蛋白(白蛋白、谷胱甘肽过氧化物酶(GPX)和硒蛋白P)中哪一种与卒中的发生有关。我们进行了一项基于人群的病例对照研究，研究对象为1992年至1994年首次中风患者，他们在中风前接受过健康检查和采血，并与30名性别、年龄、总胆固醇和采血年份相匹配的对照组。用荧光法测定血清总硒。用两个高效液相色谱柱串联分离含硒蛋白质，电感耦合等离子体质谱(ICPMS)测定硒。中风组血清硒浓度(105.2μg/L)明显低于对照组(116.5 mgg/L；p=0.054)。尽管GPx和白蛋白中的硒含量无显著差异，但卒中组的硒蛋白P显著低于对照组(54.5vs63.0μg/L；p=0.006)。多因素Logistic回归分析显示，硒蛋白P可降低卒中风险(优势比=0.28，95%可信区间：0.10~0.8)，提示硒蛋白P可能在卒中的发生发展中起重要作用。

项目成果

Dietary habits and selenium intake of residents in mountain and coastal communities in Japan

日本山区和沿海社区居民饮食习惯及硒摄入量

• 发表时间: 2004
• 期刊: Journal of Nutritional Science and Vitaminology 50
• 作者: Y.Miyazaki;H.Koyama;Y.Sasada;H.Satoh;M.Nojiri;S.Suzuki
• 通讯作者: S.Suzuki

Relationship of dietary intake of fish and non-fish selenium to serum

膳食鱼类和非鱼类硒摄入量与血清的关系

• 发表时间: 2002
• 期刊: Journal of Trace Elements in Medicine and Biology 16
• 作者: Y.Miyazaki;H.Koyama;M.Nojiri;S.Suzuki.
• 通讯作者: S.Suzuki.

中森洋介, 小山洋: "グルタチオンペルオキシターゼ活性測定法の精度管理:グルタチオンレダクターゼを用いた間接的精度管理の試み"群馬保健学紀要. 22. 63-69 (2001)

中森洋介、小山洋：“谷胱甘肽过氧化物酶活性测定方法的精度控制：利用谷胱甘肽还原酶进行间接质量控制的尝试”群马健康科学通报22. 63-69 (2001)。

Yukiko Miyazaki, Hiroshi Koyama, Masami Nojiri, Shosuke Suzuki: "Relationship of dietary intake of fish and non-fish selenium to serum lipids in Japanese rural coastal community"J.Trace Elem.Med.Biol.. 16. 83-90 (2002)

Yukiko Miyazaki、Hiroshi Koyama、Masami Nojiri、Shosuke Suzuki：“日本农村沿海社区鱼类和非鱼类硒的膳食摄入量与血脂的关系”J.Trace Elem.Med.Biol.. 16. 83-90 (2002)

蜂須賀 理絵, 小山 洋: "カドミウムの細胞障害作用に対するセレンの影響"群馬保健学紀要. 23巻(印刷中). (2003)

Rie Hachisuka、Hiroshi Koyama：“硒对镉细胞毒性作用的影响”群马健康科学通报第 23 卷（印刷中）。