Effects of selenium nutritional status for atherosclerosis prevention in hyperlipidemic mice.

硒是人体必需的微量元素，一些流行病学研究表明，血清硒水平与闭塞性血管病的发生发展呈负相关。血清中至少有三种含硒蛋白，白蛋白、细胞外谷胱甘肽过氧化物酶和硒蛋白P。HPLC-ICP-MS法分离血清样品中的硒。该方法允许一个快速和直接的色谱测定血清硒GPx，白蛋白和硒蛋白P。本研究的目的是评估这三种含硒蛋白质是最负责的血管疾病的发展，特别是动脉粥样硬化在高血脂症小鼠。将所有动物圈养在交替12小时光照和黑暗期的房间中。自由饮用自来水。制备了与Yoshida等报道的相似的缺硒饲料。日粮由圆酵母组成 关于我们 (KR酵母;东方酵母，东京，日本）（40.0%）、蔗糖（47.3%）、大豆油（8.0%）、无硒矿物质混合物AIN 93 M（3.5%）、维生素混合物AIN 93 VX（1.0%）和胆碱酒石酸氢盐（0.2%）。荧光分析表明，饲料中含有0.004毫克硒/公斤。其余的小鼠用酵母饲料喂养，其中以亚硒酸钠形式的硒为0.4mg/kg饲料。在这些硒缺乏和足够的饮食4周后，将小鼠禁食4小时，用乙醚麻醉，并通过使用含有Na 2 EDTA作为抗凝剂的塑料注射器从下腔静脉抽血来放血。通过离心分离血浆。从动物中取出整个脑、肝、肾、肌肉。所有组织样品在解剖后立即冷冻，并储存在-70 ℃下直至测定。本研究通过测定小鼠体内硒含量和GPx活性，分析硒营养状况与血管疾病尤其是动脉粥样硬化发生发展的统计学关系。少

Selenium in an essential trace element and some epidemiological studies have shown that selenium levels in serum have an inverse relationship with the development of occlusive vascular disease. There sre at least three selenium-containing proteins in serum, i.e., albumin, extracellular glutathione peroxidase and selenoprotein P. Anew method (HPLC-ICP-MS) for separating selenium in serum samples was developed by Koyama. The method allows a rapid and direct chromatographic determination of serum selenium among GPx, albumin and selenoprotein P. The objective of the present study was to assess which of these three selenium-containing proteins was the most responsible for the development of vascular diseases, especially atherosclerosis in hyperlipidemic mice. All animals were housed in a room with alternating 12-hour light and dark periods. Tap water was provided ad libitum. A selenium-deficient diet similar to that reported by Yoshida et al. was prepared. The diet consisted of torula yeast … More (KR yeast ; Oriental Yeast, Tokyo, Japan) (40.0%), sucrose (47.3%), soybean oil (8.0%), selenium-free mineral mixture AIN 93M (3.5%), vitamin mixture AIN 93VX (1.0%) and choline bitartrate (0.2%). A fluorometric analysis indicated that the diet contained 0.004 mg selenium/kg. The remaining mice were fed with the torula yeast diet with 0.4 mg selenium/kg diet as sodium selenite. After 4 weeks of these selenium deficient and sufficient diets, the mice were fasted for 4 hours, anesthetized with diethyl ether, and exsanguinated by drawing blood from the vena cava inferior using a plastic syringe containing Na2EDTA as an anticoagulant. Plasma was separated by centrifugation. The entire brain, liver, kidney, muscle were removed from the animals. All tissue samples were frozen immediately after dissection and stored at - 70 oC until assayed. We, now, are measuring the contents of selenium and the activities of GPx in these mouse samples and analyzing the statistical relationship between selenium nutritional status and the development of vascular diseases, especially atherosclerosis. Less