Effects of selenium nutritional status for atherosclerosis prevention in hyperlipidemic mice.

硒营养状况对预防高脂血症小鼠动脉粥样硬化的影响。

• 批准号: 09470107
• 负责人: KOYAMA Hiroshi
• 金额: $ 8.32万
• 依托单位: Gunma University (1998-1999)Tohoku University (1997)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470107/
• 关键词: SELENIUM; HYDROPEROXIDES; LIPID METABOLISM; ATHEROSCLEROSIS; GLUTATHIONE PEROXIDASE; PH-GPx; SELENOPROTEIN P; HPLC-ICP-MS

Selenium in an essential trace element and some epidemiological studies have shown that selenium levels in serum have an inverse relationship with the development of occlusive vascular disease. There sre at least three selenium-containing proteins in serum, i.e., albumin, extracellular glutathione peroxidase and selenoprotein P. Anew method (HPLC-ICP-MS) for separating selenium in serum samples was developed by Koyama. The method allows a rapid and direct chromatographic determination of serum selenium among GPx, albumin and selenoprotein P. The objective of the present study was to assess which of these three selenium-containing proteins was the most responsible for the development of vascular diseases, especially atherosclerosis in hyperlipidemic mice. All animals were housed in a room with alternating 12-hour light and dark periods. Tap water was provided ad libitum. A selenium-deficient diet similar to that reported by Yoshida et al. was prepared. The diet consisted of torula yeast … More (KR yeast ; Oriental Yeast, Tokyo, Japan) (40.0%), sucrose (47.3%), soybean oil (8.0%), selenium-free mineral mixture AIN 93M (3.5%), vitamin mixture AIN 93VX (1.0%) and choline bitartrate (0.2%). A fluorometric analysis indicated that the diet contained 0.004 mg selenium/kg. The remaining mice were fed with the torula yeast diet with 0.4 mg selenium/kg diet as sodium selenite. After 4 weeks of these selenium deficient and sufficient diets, the mice were fasted for 4 hours, anesthetized with diethyl ether, and exsanguinated by drawing blood from the vena cava inferior using a plastic syringe containing Na2EDTA as an anticoagulant. Plasma was separated by centrifugation. The entire brain, liver, kidney, muscle were removed from the animals. All tissue samples were frozen immediately after dissection and stored at - 70 oC until assayed. We, now, are measuring the contents of selenium and the activities of GPx in these mouse samples and analyzing the statistical relationship between selenium nutritional status and the development of vascular diseases, especially atherosclerosis. Less

硒是一种必需的微量元素，一些流行病学研究表明，血清中的硒水平与闭塞性血管疾病的发生发展呈负相关。血清中至少含有三种含硒蛋白质，即白蛋白、胞外谷胱甘肽过氧化物酶和硒蛋白P。该方法可以快速、直接地测定血清中GPX、白蛋白和硒蛋白P中的硒。本研究的目的是评估这三种含硒蛋白中哪一种是导致高脂血症小鼠血管疾病，特别是动脉粥样硬化的最主要原因。所有的动物都被关在一个12小时明暗交替的房间里。自来水是临时提供的。一种与吉田等人报告的类似的缺硒饮食。已经做好了准备。日粮由酵母…组成。更多(KR酵母；东方酵母，日本东京)(40.0%)、蔗糖(47.3%)、大豆油(8.0%)、无硒矿物质混合物An 93M(3.5%)、维生素混合物An 93VX(1.0%)和酒石酸胆碱(0.2%)。荧光分析表明，该饮食中的硒含量为0.004毫克/公斤。其余小鼠喂饲含硒0.4 mg/kg的酵母饲料。低硒充足饲料喂养4周后，禁食4h，乙醚麻醉，用含Na2EDTA抗凝剂的塑料注射器从下腔静脉抽血放血。采用离心法分离血浆。从动物身上取出了整个大脑、肝脏、肾脏和肌肉。所有组织标本在解剖后立即冷冻，并保存在-70oC直到检测。现在，我们正在测量这些小鼠样本中的硒含量和GPX活性，并分析硒营养状况与血管疾病，特别是动脉粥样硬化的发展的统计关系。较少

项目成果

Koyama H.,et al.: "Separation of selenium-contaaining proteins in human and mouse plasma using tandem high-performance liquid chromatography columns coupled with inductively coupled plasma-mass spectrometry." Analytical Biochemistry. 267(1). 84-91 (1999)

Koyama H. 等人：“使用串联高效液相色谱柱与电感耦合等离子体质谱法分离人和小鼠血浆中的含硒蛋白质。”

Koyama H., et al.: "Serum selenium distributions and the risk of stroke among rural Japanese men and women : A nested case-control analysis in Ohasama, Japan"

Koyama H. 等人：“日本农村男性和女性的血清硒分布和中风风险：日本奥哈萨马的嵌套病例对照分析”

小山 洋 他: "農村地区住民を対象とした糖負荷試験とセレン栄養状態(GPX活性)との関連"日本公衆衛生学雑誌. 46(10). 458 (1999)

Hiroshi Koyama 等人：“农村居民葡萄糖耐量测试与硒营养状况（GPX 活性）之间的关系”《日本公共卫生杂志》46（10）458（1999）。

Koyama H.et al.: "Serum Selenium distributions and the subsequent risk of stroke among rural Japanese = anested case- Control study"Epidemiology for sustainble Health (abstract book). 2. 341 (1999)

Koyama H.等人：“日本农村地区血清硒分布和随后的中风风险=麻醉病例对照研究”可持续健康流行病学（摘要书）。