Effects of selenium nutritional status for atherosclerosis prevention in hyperlipidemic mice.

硒是人体必需的微量元素，一些流行病学研究表明，血清硒水平与闭塞性血管疾病的发生呈反比关系。血清中至少存在白蛋白、细胞外谷胱甘肽过氧化物酶和硒蛋白p三种含硒蛋白，Koyama建立了一种分离血清样品中硒的新方法（HPLC-ICP-MS）。该方法可以快速、直接地色谱测定血清中GPx、白蛋白和硒蛋白p中硒的含量。本研究的目的是评估这三种含硒蛋白中哪一种对血管疾病的发展最负责，特别是在高脂血症小鼠中动脉粥样硬化。所有的动物都被安置在一个12小时交替亮暗的房间里。自来水是免费提供的。制备了类似吉田等人报道的缺硒饮食。饲粮由torula酵母…More (KR酵母；Oriental yeast, Tokyo, Japan)（40.0%）、蔗糖（47.3%）、大豆油（8.0%）、无硒矿物混合物AIN 93M（3.5%）、维生素混合物AIN 93VX（1.0%）和双酒石酸胆碱（0.2%）组成。荧光分析表明，饲料中硒含量为0.004 mg /kg。其余小鼠饲喂酵母饲料，添加0.4 mg硒/kg亚硒酸钠。在这些缺硒和足硒饮食4周后，小鼠禁食4小时，用乙醚麻醉，并使用含有Na2EDTA作为抗凝血剂的塑料注射器从下腔静脉抽血放血。离心分离血浆。整个大脑、肝脏、肾脏和肌肉都被从动物身上切除了。所有组织样品在解剖后立即冷冻，保存在- 70℃，直到检测。我们现在正在测量这些小鼠样品中硒的含量和GPx的活性，并分析硒营养状况与血管疾病特别是动脉粥样硬化的发展之间的统计关系。少

Selenium in an essential trace element and some epidemiological studies have shown that selenium levels in serum have an inverse relationship with the development of occlusive vascular disease. There sre at least three selenium-containing proteins in serum, i.e., albumin, extracellular glutathione peroxidase and selenoprotein P. Anew method (HPLC-ICP-MS) for separating selenium in serum samples was developed by Koyama. The method allows a rapid and direct chromatographic determination of serum selenium among GPx, albumin and selenoprotein P. The objective of the present study was to assess which of these three selenium-containing proteins was the most responsible for the development of vascular diseases, especially atherosclerosis in hyperlipidemic mice. All animals were housed in a room with alternating 12-hour light and dark periods. Tap water was provided ad libitum. A selenium-deficient diet similar to that reported by Yoshida et al. was prepared. The diet consisted of torula yeast … More (KR yeast ; Oriental Yeast, Tokyo, Japan) (40.0%), sucrose (47.3%), soybean oil (8.0%), selenium-free mineral mixture AIN 93M (3.5%), vitamin mixture AIN 93VX (1.0%) and choline bitartrate (0.2%). A fluorometric analysis indicated that the diet contained 0.004 mg selenium/kg. The remaining mice were fed with the torula yeast diet with 0.4 mg selenium/kg diet as sodium selenite. After 4 weeks of these selenium deficient and sufficient diets, the mice were fasted for 4 hours, anesthetized with diethyl ether, and exsanguinated by drawing blood from the vena cava inferior using a plastic syringe containing Na2EDTA as an anticoagulant. Plasma was separated by centrifugation. The entire brain, liver, kidney, muscle were removed from the animals. All tissue samples were frozen immediately after dissection and stored at - 70 oC until assayed. We, now, are measuring the contents of selenium and the activities of GPx in these mouse samples and analyzing the statistical relationship between selenium nutritional status and the development of vascular diseases, especially atherosclerosis. Less