Screening of new KL-6-associated mucins useful in clinical diagnosis of interstitial pneumonitis

筛选可用于间质性肺炎临床诊断的新型 KL-6 相关粘蛋白

• 批准号: 06670618
• 负责人: KOHNO Nobuoki
• 金额: $ 1.41万
• 依托单位: EHIME UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670618/
• 关键词: KL-6; KL-6 sub-molecules; interstitial pneumoitis; EK-7 antibody; PEP-76; モノクローナル抗体; 血清マーカー; ムチン; MUC1; クラスター9

Screening of murine monoclonal antibodies which react with novel antigenic deternlinants on KL-6 molecules was performed last year.EK-7 monoclonal antibody was finally selected for the purpose this year.EK-7 antibody was IgM and reacted with an antigenic determinant different from the determinant recognized with KL-6 antibody. The antigenic determinant was peptide portion of KL-6 molecules.The PEP-76 molecule in serum which was detected by a sandwich type of enzyme immunoasssay using EK-7 antibody as a catcher and KL-6 antibody as a tracer was designated as PEP-76. The method using 96-well EIA plates was similar to that for the measurement of KL-6.Study population was 51 healthy individuals, 13 patients with idiopathic interstitial pneumonia and 12 patients with sarcoidosis.PEP-76 level was 0.9 (0.9-3.9) U/ml [median (range)] in healthy individuals, 54 (0.9-580) U/ml in idiopathic interstitial pneumonia, and 1 (0.9-13) U/ml in sarcoidosis. Both the levels in idiopathic interstitial pneumonia and sarcoidosis were statistically higher than that in healthy individuals. when the cut-off level of PEP-76 was determined at 2.7 U/ml from the distribution of the levels of healthy individuals, the positive rate was 92% in idiopathic interstitial pneumonia and 33% in sarcoidosis. The sensitivity of PEP-76 was higher than that of KL-6 in same sera from patients with interstitial pneumonitis.These observations indicate that PEP-76 is a novel serum marker for the diagnosis of interstitial pneumonitis.

去年筛选了能与KL-6分子上的新抗原决定簇反应的鼠单克隆抗体，今年最终选择了EK-7单克隆抗体，EK-7抗体为IgM，与KL-6抗体识别的抗原决定簇不同。用EK-7抗体作捕捉剂，KL-6抗体作示踪剂，用夹心酶免疫法检测血清中PEP-76分子，命名为PEP-76。采用96孔酶免疫分析板测定PEP-76，方法与KL-6测定法相似，研究对象为51例健康人，13例特发性间质性肺炎和12例结节病患者，PEP-76水平为0.9（0.9-3.9）U/ml [中位数（范围）]，特发性间质性肺炎为54（0.9-580）U/ml，结节病为1（0.9-13）U/ml。特发性间质性肺炎和结节病患者血清中的P-gp水平明显高于健康人。根据健康人PEP-76水平的分布确定PEP-76的临界水平为2.7U/ml时，特发性间质性肺炎的阳性率为92%，结节病为33%。PEP-76对间质性肺炎的敏感性高于KL-6，提示PEP-76是诊断间质性肺炎的一种新的血清标志物。

项目成果

横山 彰仁、他: "間質性肺炎における血清トロンボモジュリンの検討" 日本胸部疾患学会雑誌. 32. 951-955 (1994)

Akihito Yokoyama 等：“间质性肺炎中血清血栓调节蛋白的检查”日本胸科疾病学会杂志 32. 951-955 (1994)。

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Yokoyama A 等人：“支气管哮喘患者的循环白细胞介素 6 水平”Am J Respir Crit Care Med。

Yokoyama A,Kohno N,Fujino S,Hamada H,Inoue Y,Fujioka S,Ishida S,Hiwada K.: "Circulating interleukin-6 levels in patients with bronchial asthma" Am J Respir Crit Care Med. 151. 1354-1358 (1995)

Yokoyama A、Kohno N、Fujino S、Hamada H、Inoue Y、Fujioka S、Ishida S、Hiwada K.：“支气管哮喘患者的循环白细胞介素 6 水平”Am J Respir Crit Care Med。

Yokoyama A,et al.: "Evaluation of soluble interleukin-6 receptor concentration in serum and epithelial lining fluid from patients with interstitial lung diseases" Clin Exp Immunol. 100. 325-329 (1995)

Yokoyama A 等人：“间质性肺病患者血清和上皮内衬液中可溶性白细胞介素 6 受体浓度的评估”Clin Exp Immunol。

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