Anti KL-6 antibody enhances the effects of molecular targeted drug

抗KL-6抗体增强分子靶向药物疗效

• 批准号: 23659433
• 负责人: KOHNO Nobuoki
• 金额: $ 2.25万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659433/
• 关键词: KL-6; 分子標的治療薬; 肺癌

The aim of the present study was to investigate the additive effect of anti-KL-6 antibody on the therapeutic antibody-induced ADCC (Antibody-Dependent Cellular-Cytotoxicity) activity. The expression of HER2 and KL-6 were measured using FACS analysis in 4 cancer cell lines (SK-BR-3, ZR75-1, PC3, YMB-S), and both HER2 and KL-6 were highly expressed in YMB-S cells. Accordingly, the effects of anti KL-6 antibody on the ADCC activity of PBMCs evoked by Trastuzumab were examined using Cr-51 release assay. Enhanced cytotoxicity was observed in an effector-to-target ratio manner when YMB-S cells were treated with anti-KL-6 antibody. These findings indicate that anti KL-6 antibody can enhance the therapeutic antibody-induced ADCC activity mediated by mononuclear cells.

本研究的目的是研究抗kl -6抗体对治疗性抗体诱导的ADCC（抗体依赖性细胞毒性）活性的加性作用。采用FACS法检测4种肿瘤细胞系（SK-BR-3、ZR75-1、PC3、YMB-S）中HER2和KL-6的表达，发现HER2和KL-6在YMB-S细胞中均高表达。因此，采用Cr-51释放法检测抗KL-6抗体对曲妥珠单抗诱导的PBMCs ADCC活性的影响。抗kl -6抗体作用于YMB-S细胞后，细胞毒性呈效靶比增强。上述结果表明，抗KL-6抗体可增强单核细胞介导的治疗性抗体诱导的ADCC活性。

项目成果

Comparison of serum KL-6 versus bronchoalveolar lavage neutrophilia for the diagnosis of bronchiolitis obliterans in lung transplantation.

血清 KL-6 与支气管肺泡灌洗中性粒细胞增多症诊断肺移植闭塞性细支气管炎的比较。

• DOI: 10.1016/j.healun.2011.07.010
• 发表时间: 2011
• 期刊: J Heart Lung Transplant
• 作者: Ohshimo S;Bonella F;Sommerwerck U;Teschler H;Kamler M;Jakob HG;Kohno N;Guzman J;Costabel U.
• 通讯作者: Costabel U.

Serum KL-6 is a useful diagnostic and prognostic biomarker in idiopathic interstitial pneumonia in German patients.

血清 KL-6 是德国患者特发性间质性肺炎的有用诊断和预后生物标志物。

• 发表时间: 2011
• 作者: Horimasu Y;Ohshimo S;Ishikawa N;Tanaka S;Yoshioka K;Bonella F;Hattori N;Kohno N;Guzman J;Costabel U.
• 通讯作者: Costabel U.

Differences in serum SP-D levels between German and Japanese subjects are associated with SFTPD gene polymorphisms.

• DOI: 10.1186/1471-2350-15-4
• 发表时间: 2014-01-08
• 期刊: BMC medical genetics
• 作者: Horimasu Y;Hattori N;Ishikawa N;Tanaka S;Bonella F;Ohshimo S;Guzman J;Costabel U;Kohno N
• 通讯作者: Kohno N

Discovery and clinical application of KL-6, a circulating biomarker for interstitial lung diseases

间质性肺疾病循环生物标志物KL-6的发现及临床应用

• 发表时间: 2013
• 作者: Miyake K;Tani K;Kakiuchi S;Suzuka C;Toyoda Y;Kishi J;Tezuka T;Yuasa S;Hanibuchi M;Aono Y;Nishioka Y;Sone S;Kohno N
• 通讯作者: Kohno N

Difference of serum SP-D levels between German and Japanese are associated with SFTP gene polymorphisms.

德国和日本血清SP-D水平的差异与SFTP基因多态性有关。

• 发表时间: 2013
• 作者: Horimasu Y;Hattori N;Ishikawa N;Tanaka S;Bonella F;Ohshimo S;Guzman J;Costabel U; KohnoN.
• 通讯作者: KohnoN.