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The Study on KL-6 as Morbidity Ornamentation Factor and Prognosis Factor of Idiopathic Interstitial Pneumonia.

KL-6作为特发性间质性肺炎发病影响因素和预后因素的研究。

基本信息

批准号：
14370197
负责人：
KOHNO Nobuoki
金额：
$ 8.83万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370197/
关键词：
MUC1 idiopathic interstitial pneumonia lung cancer prognostic factor MUC1 polymorphism tandem repeat embryo lung primates 突発性間質性肺炎特発性間質性 KL-6 MUCl 肺胞II型上皮 Protease サイトカイン線維芽細胞ヒト肺胞上皮細胞アポトーシス

项目摘要

The significance of serum KL-6 level as a clinical prognosis factor in patients with idiopathic interstitial pneumonia or lung cancer and the biological effects of purified KL-6 molecule on the behavior of epithelial cells were examined in 2005.As a prognostic factor of idiopathic interstitial pneumonia, the total results of a multi-institutional co-operative study, which had been studied for several years, were submitted. The extra-cellular domain of KL-6 (MUC1) has the repetitive arrangement of 60 bp (s)(tandem repeat), and the frequency of the tandem repeat shows polymorphism. Whether or not the frequency of the tandem repeat has effects on the survival of lung cancer patients was examined. As the results, the length of MUC1 was significantly longer in adenocarcinoma patients than healthy controls, and adenocarcinoma patients with longer MUC1 significantly showed poorer survival than those with shorter MUC1. The expression level of MUC1 was examined in the fetal lung using immunohistochemistry (the ABC method). Seven monoclonal antibodies which react with different epitopes on MUC1 were used. We found that there were different expression patterns among the antigenic determinants of the antibodies. Both KL-6 and NCL-MUC1 were found throughout the examined embryonic period (from 13 to 28 weeks). On the other hand, HFMG2 and H9 showed positive staining on the tip part of airways of the early embryonic lung, and they disappered from the peripheral bronchi, but were only found on the alveolar epithelial cells. To investigate the possibility of the existence of KL-6 in the lung from 12 different species of primate, the serum level of KL-6 was studied. KL-6 existed in sera from orangutan, chimpanzee and gorilla, but not in those of Japanese monkey. It turned out that KL-6 were gained in the process of evolution and it exists in humans.

2005年对血清KL-6水平作为特发性间质性肺炎或肺癌患者临床预后因子的意义及纯化的KL-6分子对上皮细胞生物学行为的影响进行了研究。作为特发性间质性肺炎的预后因子，多机构合作研究已进行了多年。KL-6胞外区(MUc1)具有60bp的重复排列(S)(串联重复)，串联重复频率呈多态。研究了串联重复频率是否对肺癌患者的生存有影响。结果表明，腺癌患者的MUC1长度明显长于健康对照组，而MUC1长度较长的腺癌患者的生存率明显低于MUC1较短的患者。免疫组织化学ABC法检测胎鼠肺组织中MUC1的表达水平。使用7种与MUC1不同表位反应的单抗。我们发现抗体的抗原决定簇之间存在着不同的表达模式。KL-6和NCL-MUC1在整个胚胎期(13~28周)均有表达。而HFMG2和H9在早期胚胎肺的气道顶端呈阳性染色，在周围支气管消失，但仅见于肺泡上皮细胞。为探讨12种灵长类动物肺组织中存在KL-6的可能性，对12种灵长类动物血清中KL-6水平进行了研究。KL-6存在于猩猩、黑猩猩和大猩猩的血清中，而不存在于日本猴的血清中。事实证明，KL-6是在进化过程中获得的，它存在于人类体内。