The Study on KL-6 as Morbidity Ornamentation Factor and Prognosis Factor of Idiopathic Interstitial Pneumonia.

2005年，我们检测了血清KL-6水平作为特发性间质性肺炎或肺癌患者的临床预后因子的意义，以及纯化的KL-6分子对上皮细胞行为的生物学效应。作为特发性间质性肺炎的预后因子，我们提交了一项多机构合作研究的总体结果。KL-6胞外区（MUC 1）具有60 bp的重复序列（串联重复序列），其频率呈现多态性。研究了串联重复序列的频率是否对肺癌患者的生存有影响。结果，腺癌患者的MUC 1长度显著长于健康对照，MUC 1较长的腺癌患者的生存率显著低于MUC 1较短的腺癌患者。采用免疫组化ABC法检测胎肺MUC 1的表达水平。使用与MUC 1上的不同表位反应的七种单克隆抗体。我们发现，有不同的表达模式之间的抗原决定簇的抗体。KL-6和NCL-MUC 1在整个胚胎期（13 - 28周）都有表达。另一方面，HFMG 2和H9在早期胚胎肺的气道尖端部分显示阳性染色，并且它们从外周支气管消失，但仅在肺泡上皮细胞上发现。为探讨KL-6在12种不同灵长类动物肺组织中存在的可能性，研究了血清中KL-6的水平。KL-6在猩猩、黑猩猩和大猩猩血清中存在，而在日本猴血清中不存在。原来KL-6是在进化过程中获得的，它存在于人类体内。

The significance of serum KL-6 level as a clinical prognosis factor in patients with idiopathic interstitial pneumonia or lung cancer and the biological effects of purified KL-6 molecule on the behavior of epithelial cells were examined in 2005.As a prognostic factor of idiopathic interstitial pneumonia, the total results of a multi-institutional co-operative study, which had been studied for several years, were submitted. The extra-cellular domain of KL-6 (MUC1) has the repetitive arrangement of 60 bp (s)(tandem repeat), and the frequency of the tandem repeat shows polymorphism. Whether or not the frequency of the tandem repeat has effects on the survival of lung cancer patients was examined. As the results, the length of MUC1 was significantly longer in adenocarcinoma patients than healthy controls, and adenocarcinoma patients with longer MUC1 significantly showed poorer survival than those with shorter MUC1. The expression level of MUC1 was examined in the fetal lung using immunohistochemistry (the ABC method). Seven monoclonal antibodies which react with different epitopes on MUC1 were used. We found that there were different expression patterns among the antigenic determinants of the antibodies. Both KL-6 and NCL-MUC1 were found throughout the examined embryonic period (from 13 to 28 weeks). On the other hand, HFMG2 and H9 showed positive staining on the tip part of airways of the early embryonic lung, and they disappered from the peripheral bronchi, but were only found on the alveolar epithelial cells. To investigate the possibility of the existence of KL-6 in the lung from 12 different species of primate, the serum level of KL-6 was studied. KL-6 existed in sera from orangutan, chimpanzee and gorilla, but not in those of Japanese monkey. It turned out that KL-6 were gained in the process of evolution and it exists in humans.