The Study on KL-6 as Morbidity Ornamentation Factor and Prognosis Factor of Idiopathic Interstitial Pneumonia.

2005年研究了血清KL-6水平作为特发性间质性肺炎或肺癌患者临床预后因素的意义以及纯化的KL-6分子对上皮细胞行为的生物学效应。作为特发性间质性肺炎的预后因素，提交了一项多机构合作研究的总结果，该研究已进行了数年。KL-6 （MUC1）的胞外结构域具有60 bp (s)的重复排列（串联重复），且串联重复的频率具有多态性。研究了串联重复序列的频率是否对肺癌患者的生存有影响。结果表明，腺癌患者MUC1的长度明显长于健康对照组，且MUC1长度较长的腺癌患者的生存率明显低于MUC1长度较短的腺癌患者。采用免疫组织化学（ABC法）检测MUC1在胎儿肺中的表达水平。使用7种与MUC1不同表位反应的单克隆抗体。我们发现在抗体的抗原决定因子中有不同的表达模式。KL-6和NCL-MUC1在胚胎期（13 ~ 28周）均有表达。另一方面，HFMG2和H9在早期胚胎肺气道尖端部分呈阳性染色，在外周支气管中消失，仅在肺泡上皮细胞中发现。为探讨12种不同灵长类动物肺组织中存在KL-6的可能性，测定了血清中KL-6的含量。猩猩、黑猩猩和大猩猩血清中均存在KL-6，日本猴血清中不存在KL-6。原来KL-6是在进化过程中获得的，存在于人类体内。

The significance of serum KL-6 level as a clinical prognosis factor in patients with idiopathic interstitial pneumonia or lung cancer and the biological effects of purified KL-6 molecule on the behavior of epithelial cells were examined in 2005.As a prognostic factor of idiopathic interstitial pneumonia, the total results of a multi-institutional co-operative study, which had been studied for several years, were submitted. The extra-cellular domain of KL-6 (MUC1) has the repetitive arrangement of 60 bp (s)(tandem repeat), and the frequency of the tandem repeat shows polymorphism. Whether or not the frequency of the tandem repeat has effects on the survival of lung cancer patients was examined. As the results, the length of MUC1 was significantly longer in adenocarcinoma patients than healthy controls, and adenocarcinoma patients with longer MUC1 significantly showed poorer survival than those with shorter MUC1. The expression level of MUC1 was examined in the fetal lung using immunohistochemistry (the ABC method). Seven monoclonal antibodies which react with different epitopes on MUC1 were used. We found that there were different expression patterns among the antigenic determinants of the antibodies. Both KL-6 and NCL-MUC1 were found throughout the examined embryonic period (from 13 to 28 weeks). On the other hand, HFMG2 and H9 showed positive staining on the tip part of airways of the early embryonic lung, and they disappered from the peripheral bronchi, but were only found on the alveolar epithelial cells. To investigate the possibility of the existence of KL-6 in the lung from 12 different species of primate, the serum level of KL-6 was studied. KL-6 existed in sera from orangutan, chimpanzee and gorilla, but not in those of Japanese monkey. It turned out that KL-6 were gained in the process of evolution and it exists in humans.