Basic study for genetic therapy by analysis of intracellular signal transduction in thyroid carcinoma cell
甲状腺癌细胞胞内信号转导分析的基因治疗基础研究
基本信息
- 批准号:06671039
- 负责人:
- 金额:$ 1.34万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1994
- 资助国家:日本
- 起止时间:1994 至 1995
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1) Previous epidemiology study shows thyroid tissue is prone to radiation induced tumorigenesis. To investigate whether the tissue specific intracellular responses to irradiation in thyroid cells is existed or not, we have done the several studies. To this purpose, we used primary cultured thyroid cell that has normal genetic background, and some cell lines established from thyroid carcinoma that have genetic defects, for instance, p53 mutation. The survival rates after 2 Gy irradiation were 18% and >58% in primary thyroid cell and thyroid carcinoma cell line, respectively. G1 arrest after irradiation has been shown only in primary thyroid cell but not in thyroid carcinoma cells. It suggests G1arrest is depend on p53 status. We can find apoptosis is rare event in thyroid cells after radiation exposure. Next, we checked the change of the expression of the p53 down stream genes (p21/WAF1, Bax and FAS/APO1) after radiation. Although the expression of p21/WAF1 gene was increased, no change of expression was observed in Bax and FAS/APO gene. These results suggest there is thyroid tissue specific response after irradiation. We published these data on Cancer Res.2) We developed semi-automated measurement apparatus of urinary iodine. We published these data on Clinical Chemistry.
1)以往的流行病学研究表明,甲状腺组织容易发生辐射诱发的肿瘤。为了探讨甲状腺细胞是否存在组织特异性的细胞内辐射反应,我们进行了几项研究。为此,我们使用了具有正常遗传背景的原代培养甲状腺细胞,以及从甲状腺癌中建立的具有遗传缺陷(例如,p53突变)的细胞系。原代甲状腺细胞和甲状腺癌细胞株经2戈伊照射后的存活率分别为18%和>58%。照射后仅原代甲状腺细胞出现G1期阻滞,而甲状腺癌细胞未见G1期阻滞。提示G1期阻滞依赖于p53状态。我们发现放射线照射后甲状腺细胞很少发生凋亡。然后检测p53下游基因(p21/WAF 1、Bax和FAS/APO 1)在辐射后的表达变化。p21/WAF 1基因表达增强,Bax和FAS/APO基因表达无明显变化。结果提示,照射后甲状腺组织有特异性反应。2)研制了半自动尿碘测定仪。我们在临床化学上发表了这些数据。
项目成果
期刊论文数量(48)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Yamashita S: "A report on the 1994 Chernobyl Sasakawa Project Workshop." Sasakawa Memorial Health Foundation, 322 (1994)
Yamashita S:“1994 年切尔诺贝利笹川项目研讨会的报告。”
- DOI:
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- 影响因子:0
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- 通讯作者:
Maeda, H et.al.: "A single missense mutation in codon 918 of the RET proto-oncogene in sporadic medullary thyroid carcinomas" Endocrine J.42. 245-250 (1995)
Maeda, H 等人:“散发性甲状腺髓样癌中 RET 原癌基因密码子 918 中的单一错义突变”Endocrine J.42。
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- 影响因子:0
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- 通讯作者:
Matuso T et al: "Inhibition of epidermal growth factor binding system by ionizing radiation in A431 human squamous carcinoma cells." Cancer Lett. 89. 153-159 (1995)
Matuso T 等人:“电离辐射对 A431 人鳞状癌细胞中表皮生长因子结合系统的抑制。”
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- 影响因子:0
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Hosoda,C.: "Establishment and characterization of a malignant epitheloid hmangioendothelioma from mouse thyroid tumor." Endocrine J.42. 341-350 (1995)
Hosoda,C.:“小鼠甲状腺肿瘤恶性上皮样血管内皮瘤的建立和表征。”
- DOI:
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- 影响因子:0
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- 通讯作者:
Tsuda K,etal.: "Automated measurement of urinary iodine with use of ultraviolet irradiation" Clin Chem.41. 581-585 (1995)
Tsuda K,etal.:“利用紫外线照射自动测量尿碘”Clin Chem.41。
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- 影响因子:0
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{{ truncateString('NAMBA Hiroyuki', 18)}}的其他基金
Thrombomodulins's effects in ocular tissue and their clinical administration.
血栓调节蛋白对眼组织的影响及其临床给药。
- 批准号:
23791964 - 财政年份:2011
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
In vivo model mouse study to examine the effect of alteration of endogenous endocrine status in thyroid tumorigenesis
体内模型小鼠研究探讨内源性内分泌状态改变对甲状腺肿瘤发生的影响
- 批准号:
18591030 - 财政年份:2006
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular Epidemiological Study of Thyroid Disease in Radiation Contaminated Area
辐射污染地区甲状腺疾病的分子流行病学研究
- 批准号:
15256004 - 财政年份:2003
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Thyroid cancer study for establishment of prognostic marker and molecular targeting therapy using gene mutation analysis.
使用基因突变分析建立预后标志物和分子靶向治疗的甲状腺癌研究。
- 批准号:
15590981 - 财政年份:2003
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Comprehensive Study of Carcinogemesis and Molecular Target Therapy in Thyroid Cancer
甲状腺癌致癌与分子靶向治疗的综合研究
- 批准号:
15390295 - 财政年份:2003
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Competitive analysis of radiation induced gene expression between thyroid cell and fibroblast
甲状腺细胞与成纤维细胞辐射诱导基因表达的竞争分析
- 批准号:
09680529 - 财政年份:1997
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of signal transduction in thyroid cancer cell
甲状腺癌细胞信号转导分析
- 批准号:
04671490 - 财政年份:1992
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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识别受辐射甲状腺中癌症风险的辐射特征
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16K08714 - 财政年份:2016
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辐射诱发甲状腺癌模型的建立及抗氧化剂对甲状腺DNA损伤的抑制作用分析
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26870440 - 财政年份:2014
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25460470 - 财政年份:2013
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