Competitive analysis of radiation induced gene expression between thyroid cell and fibroblast

1)甲状腺是辐射敏感器官之一。在广岛和长崎或切尔诺贝利地区的HIBAKUSHA筛查中观察到甲状腺肿瘤的高发病率。为了研究辐射敏感性的差异，我们比较分析了甲状腺细胞和成纤维细胞在辐射暴露后JNK的活性。结果发现，放射线照射（2-5Gy）后，甲状腺细胞JNK活性增强2-3倍，而成纤维细胞则无明显增强。进一步的研究表明辐射诱导的JNK活性是通过蛋白激酶C调节的。这一结果已发表在BBRC（244，1998）。2）为了研究辐射诱导表达的基因，我们用系列分析基因表达方法分析了基因库。分析了来自未暴露电池的12016个标签和来自10戈伊暴露电池的14020个标签。我们进一步继续分析暴露细胞和未暴露细胞的标签。

1) Thyroid gland is known to be one of radiation sensitive organs. High incidence of thyroid tumor is observed in the screening of HIBAKUSHA in Hiroshima and Nagasaki or Chernobyl region. To examine the difference of radiation sensitivity, we comparatively analyzed JNK activity following exposure to radiation between thyroid cells and fibroblasts. We found the JNK activity was 2-3 fold induction in thyroid cells following radiation exposure (2-5Gy), but no induction in fibroblasts. Further study revealed the radiation-induced JNK activity was mcdiated by protein kinase C.The paper described the results was published in BBRC (244, 1998).2) To investigate the genes of which expressions are induced by irradiation, we analyzed the repertoire of the genes using Serial Analysis Gene expression method. 12016 tags from no- exposed cell and 14020 tags from 10 Gy exposed cells have been analyzed. We further continue to analyze the tags of exposed cells and non-exposed cells.