Antitumor activity by intracellular hyperthermia using magnetite

使用磁铁矿进行细胞内热疗的抗肿瘤活性

• 批准号: 11671365
• 负责人: WAKABAYASHI Toshihiko
• 金额: $ 2.18万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671365/
• 关键词: Hyperthermia; Magnetite; Brain tumor; Liposome; Immunoreaction; Hysteresis loss; 正電荷リポソーム; 熱ショックタンパク質; 免疫

Intratumoral effect of Carboxymethylcellulose(CMC)-magnetite, which newly produced, under hyperthermic condition was demonstrated in this study. CMC and magnetite particles were mingled and a stick type magnetite was developed to obtain higher hyperthermic effect. This type magnetite was injected stereotactically into the T9 glioma in the rat brain and these rats were applied to the high frequent magnetic field. Stick type CMC-magnetite spread among the tumor tissue after application into the magnetic field. This static magnetic field application also stimulates the magnetite particles and could produce heat enough to induce tumor necrosis. This study of hyperthermia also demonstrated assembling macrophages and lymphocytes just around the magnetite, which indicates possibility of heated magnetite to induce activation of immunoreaction in the brain

本研究证实了新生成的羧甲基纤维素(CMC)磁铁矿在高温条件下的瘤内效应。将CMC与磁铁矿颗粒混合，研制出棒状磁铁矿，以获得较高的热效应。将这种类型的磁铁矿立体定向注射到大鼠脑内T9胶质瘤内，并对其施加高频磁场。棒状CMC-磁铁矿作用于磁场后弥漫于肿瘤组织中。这种静态磁场的应用也会刺激磁铁矿颗粒，并可能产生足够的热量来导致肿瘤坏死。这项关于高温的研究还表明，巨噬细胞和淋巴细胞聚集在磁铁矿周围，这表明加热的磁铁矿可能诱导脑内免疫反应的激活。

项目成果

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Ito A 等人：“Augmentation of MHC I类抗原呈递通过热休克蛋白表达通过热疗”CancerImmunolImmunother..50.515-522(2001)

Wakabayashi T, Ohno T, Takemura A, Hatano H, Hatano N, Kajita Y, Yoshida J,: "Analysis of suitable hyperthermic condition of stick type magnetite for malignant glioma"Neuroimmunological Research. 11. 207-210 (1999)

Wakabayashi T，Ohno T，Takemura A，Hatano H，Hatano N，Kajita Y，Yoshida J，：“棒型磁铁矿治疗恶性胶质瘤的适宜高温条件分析”神经免疫学研究。

Wakabayashl T, Ohno T, Yoshida J, Yanase M, Shinkai M, Honda H, Takahashi M, KobayasM T: "Specific imaging and' intracellular hyperthermia for brain tumor using magnetite particle."Neuroimmunological Research. 11. 1401-145 (1999)

Wakabayashl T、Ohno T、Yoshida J、Yanase M、Shinkai M、Honda H、Takahashi M、KobayasM T：“使用磁铁矿颗粒对脑肿瘤进行特异性成像和细胞内热疗。”神经免疫学研究。

Ohno T, Takemura I M, Honda H,: "Intratumoral distribution of gelated type and stick type CMC-magnetite under hyperthermia for malignant glioma."Neuroimmunological Research. 11. 211-215 (1999)

Ohno T、Takemura I M、Honda H，：“恶性胶质瘤热疗下凝胶型和棒型 CMC-磁铁矿的瘤内分布。”神经免疫学研究。

Shinkai M, et al.: "Targeting hyperthermia for renal cell carcinorna using human MN antigen specific magnetoliposomes"Jpn J. Cancer Res.. 92. 1138-1145 (2001)

Shinkai M 等：“使用人 MN 抗原特异性磁脂质体靶向热疗治疗肾细胞癌”Jpn J. Cancer Res.. 92. 1138-1145 (2001)