Induction of anti-tumor immunity by intracellular hyperthermia using magnetite

利用磁铁矿进行细胞内高温诱导抗肿瘤免疫

• 批准号: 14370430
• 负责人: WAKABAYASHI Toshihiko
• 金额: $ 8.32万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370430/
• 关键词: Hyperthermia; Magnetite; Brain tumor; Liposome; Anti-tumor immunity; HSP70; Magnetic field

Magnetite particles of nanometer size are involved in an increasing number of biological and medical applications. The unique feature of magnetite particles is their reaction to a magnetic force. Hysteresis loss in the alternative magnetic field is a very important feature of magnetic particles, because it enables effective hyperthermia. Using magnetite cationic liposomes (MCLs), induction of antitumor immunity to glioma by intracellular hyperthermia was investigated in the central nervous system. MCLs, which have a positive surface charge, were used as heating mediators for intracellular hyperthermia. T-9 rat glioma tissue was formed subcutaneously on femur of female F344 rats, and MCLs was injected into the tumor mass. After that, the rats were received irradiation with an alternating magnetic field (AMP). As a result, the pathological investigation demonstrated effectiveness in attacking glioma immunologically even in the brain and significant prolonging of the survival time. In conclusion, this method of hyperthermic treatment may induce antitumor immunity and can effect on glioma even in the central nervous system as an antitumor vaccination.

纳米大小的磁铁矿颗粒越来越多地应用于生物和医学领域。磁铁矿粒子的独特之处在于它们对磁力的反应。磁滞损失在交替磁场是磁性粒子的一个非常重要的特征，因为它可以实现有效的热疗。利用磁铁矿阳离子脂质体（mcl），研究了中枢神经系统细胞内热疗诱导胶质瘤抗肿瘤免疫的作用。mcl具有正的表面电荷，被用作细胞内热疗的加热介质。雌性F344大鼠股骨皮下形成T-9大鼠胶质瘤组织，肿瘤块内注射mcl。然后，用交变磁场（AMP）照射大鼠。结果，病理调查显示免疫攻击胶质瘤的有效性，甚至在大脑和显著延长生存时间。综上所述，这种热疗方法可以诱导抗肿瘤免疫，甚至可以作为抗肿瘤疫苗对中枢神经系统的胶质瘤产生作用。

项目成果

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