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Fundamental study of neuronal cell differentiation system by transfection with glial fibrillary acidic protein gene.

胶质纤维酸性蛋白基因转染神经细胞分化系统的基础研究。

基本信息

批准号：
04670859
负责人：
WAKABAYASHI Toshihiko
金额：
$ 1.09万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670859/
关键词：
GFAP Gene transfection,Brain tumor

项目摘要

Glial fibrillary acidic protein (GFAP) is one of the intermediate filaments which was found in mature normal astrocytes and differentiated glioma cells It seems to stabilize the astrocyte's cytoskeleton and help maintain astrocyte cell shape together with other cytoskeletal components such as microfilament and microtubule, however its tissue-specific role is not clear. To verify the effect of GAFP expression in brain tumor cell, transfer of GFAP gene into human medulloblastoma cell line (DAOY-1) which dose not express GFAP was performed by means of liposomes. After tranfection, we studied the alteration of chracteristics in GFAP transfected cells. Cell growth, morphology and sensitivity of cytokine or anticancer drugs were compared between GFAP transfected and non-transfected cells. As a result, the GFAP gene transfected cells demonstrated that, 1) Growth inhibition, 2) Increase of sensitivity to some anticancer drugs such as ACNU and Cisplatinum, 3) High expression of tumor related cell surface antigen such as G-22, which is known as one of the intercellular adhesion molecule, 4) although morphological changes was not apparent. These results suggest that GFAP gene transfected cell has a possibility to be more sensitive for the anticancer drugs, and it is expected the clinical application for the treatment of malignant brain tumors.

胶质细胞酸性蛋白（GFAP）是一种存在于成熟的正常星形胶质细胞和分化的胶质瘤细胞中的中间纤维，它似乎与其他细胞骨架成分如微丝和微管一起稳定星形胶质细胞的细胞骨架，帮助维持星形胶质细胞的形状，但其组织特异性作用尚不清楚。为了验证GFAP基因在脑肿瘤细胞中表达的效果，我们用脂质体将GFAP基因导入不表达GFAP的人髓母细胞瘤细胞系（DAOY-1）。转染GFAP后，我们观察了GFAP转染细胞特性的变化。比较GFAP转染和未转染细胞的生长、形态和细胞因子或抗癌药物的敏感性。结果显示，GFAP基因转染的细胞表现出：1）生长抑制，2）对某些抗癌药物如ACNU和顺铂的敏感性增加，3）肿瘤相关细胞表面抗原如G-22的高表达，G-22是已知的细胞间粘附分子之一，4）尽管形态学变化不明显。提示GFAP基因转染的细胞对抗癌药物有更高的敏感性，有望在临床上应用于恶性脑肿瘤的治疗。