Effective induction of antiglioma cytotoxic T cells by coadministration of heat-induction antigen by means of bio-nanoparticles dendritic cells

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As it is well known that heat-induction particles enhance the stimulatory activity of dendritic cells (DCs), we hypothesized that DCs in the presence of the heat-induction antigens would provide particularly effective antitumor activity against glioma cells by stimulating cytotoxic T cell. As a fundamental study, a rat glioma cell line T-98 was heated with bio-nano particles, magnetite, under the alternative magnetic field to continue the adequate concentration level of heat-induction antigens around the tumor cells, then cocultured with syngeneic bone marrow-derived DCs and naive splenic T cells. The T-98 cells were almost completely killed cocluture with DCs and T cells, and strong tumor-specific cytotoxic T lymphocyte (CTL) activity was induced. As a pre-clinical study, human DCs accumulated from peripheral blood CD14 positive monocyte was activated with IL-2 and GM-CSF and analyzed the expression of cytokine level in culture. Remarkable elevation of expression level of interleukin-12 and IFN-gamma could be observed in culture which may activate T lymphocytes. These result suggest that heat-induction antigen would stimulate DCs and induce antitumor immunity against glioma by coculturing with T lymphocytes which may act as displaying lytic activity against glioma.