Environmental Control of Patients with Asthma (Emphasis on Fungal Allergy)

对哮喘患者过敏原白色念珠菌的分析、交叉反应性及室内真菌菌群进行了研究。皮肤点刺试验证实白色念珠菌烯醇化酶(CAE)的致敏性。CAE与酿酒酵母有一些交叉反应表位。提示CAE上的IgE结合表位在蛋白的C末端附近存在。对隐球菌属、红酵母属和念珠菌属粗制抗原的IgE抗体进行了检测。在真菌过敏患者的血清中检测到。从室内空气样品中分离到哮喘患者以念珠菌、隐球菌属和红酵母属为主。室内空气颗粒物(2-5微米)是室外空气颗粒物的10倍。在房屋灰尘中经常检测到肺吸虫和尘螨。对哮喘患者进行环境调查时，需对室内空气中真菌菌群、颗粒物及室内粉尘进行全面检查，并对家庭进行除湿、清洗(含空调过滤器)、存放干粮、使用福田、使用取暖器具、清理落叶等家庭护理。

Analysis of Candida albicanof allergen, its crossreactivity and indoor fungal flora in patients with asthma were studied.1. Allergenic potency of Candida albicansenolase (CAE) was confirmed using a skin-prick test.2. CAE shares some crossreacting epitopes with Saccharomyces cerevisiaeenolase.3. It is suggested that IgE binding epitopes on CAE exist near the C-terminal of the protein.4. The IgE antibodies for crude antigens prepared from Cryptococcus spp., Rhodotorula spp.and Candida spp. were detected in the patients'sera with fungal allergy.5. From indoor air sample, Candida SPP., Cryptococcus spp.and Rhodotorula spp.were dominantly isolated in patients with Asthma.6. Particle counts (2-5 mum) of indoor air were ten times of the one in outdoor air.7. Trogium pulsatorium as well as mites were frequently detected in house dust.8. We found that the survey for environment of the patients with Asthma need total examination of fungal flora and particles in indoor air, and mite in house dust.Home Care for family were carried out about dehumidification of indoor air, cleaning (including filters of an air conditioner), storage of dry foods, using Futon, using heating apparatus and cleaning fallen leaves etc..