Environmental Control of Patients with Asthma (Emphasis on Fungal Allergy)

哮喘患者的环境控制（重点关注真菌过敏）

• 批准号: 06672320
• 负责人: ISHIGURO Ayako
• 金额: $ 1.34万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06672320/
• 关键词: Environmental Control; Home Care; Candida albicans; Asthma; IgE antibody; Enolase; Cell Wall Mannan; Crossreactivity; エノラーゼ; 細胞壁マンナン; イムノブロット; エピトープ

Analysis of Candida albicanof allergen, its crossreactivity and indoor fungal flora in patients with asthma were studied.1. Allergenic potency of Candida albicansenolase (CAE) was confirmed using a skin-prick test.2. CAE shares some crossreacting epitopes with Saccharomyces cerevisiaeenolase.3. It is suggested that IgE binding epitopes on CAE exist near the C-terminal of the protein.4. The IgE antibodies for crude antigens prepared from Cryptococcus spp., Rhodotorula spp.and Candida spp. were detected in the patients'sera with fungal allergy.5. From indoor air sample, Candida SPP., Cryptococcus spp.and Rhodotorula spp.were dominantly isolated in patients with Asthma.6. Particle counts (2-5 mum) of indoor air were ten times of the one in outdoor air.7. Trogium pulsatorium as well as mites were frequently detected in house dust.8. We found that the survey for environment of the patients with Asthma need total examination of fungal flora and particles in indoor air, and mite in house dust.Home Care for family were carried out about dehumidification of indoor air, cleaning (including filters of an air conditioner), storage of dry foods, using Futon, using heating apparatus and cleaning fallen leaves etc..

对哮喘患者白色念珠菌过敏原、交叉反应性及室内真菌菌群进行了分析。皮肤点刺试验证实了白色念珠菌酵素醇酶（CAE）的致敏效力。CAE与酿酒酵母菌烯醇酶具有一些交叉反应的表位。这表明CAE上的IgE结合表位存在于该蛋白的c端附近。在真菌过敏患者血清中检测隐球菌、红酵母和念珠菌粗抗原的IgE抗体。从哮喘患者的室内空气样本中，主要分离到念珠菌、隐球菌和红酵母。室内空气微粒数（2 ~ 5 μ m）是室外空气微粒数的10倍。在室内粉尘中常检出脉状曲螨和螨虫。我们发现哮喘患者的环境调查需要全面检查室内空气中的真菌菌群和颗粒，以及室内灰尘中的螨虫。对家庭进行了室内空气除湿、清洁（包括空调过滤器）、储存干粮、使用蒲团、使用加热器具和清洁落叶等家庭护理。

项目成果

神戸俊夫: "カンジダアルビカンス細胞壁マンナンのアレルゲン性に関する研究" 日本細菌学会誌. 51. 104- (1996)

神户敏夫：“白色念珠菌细胞壁甘露聚糖致敏性的研究”日本细菌学会杂志 51. 104- (1996)。

A.Ishiguro: "YEAST FLORA IN THE INDOOR AIR/HOUSE DUST AND ALLERGENIC ACTIVITY OF YEAST SPECIES" The 7th International Conference on Indoor Air Quality and Climate (Abstract). (In press). 6

A.Ishiguro：“室内空气/房屋灰尘中的酵母菌群和酵母菌种的过敏活性”第七届室内空气质量和气候国际会议（摘要）。

Ishiguro Ayako: "YEAST FLORA IN THE INDOOR AIR/HOUSE DUST AND ALLERGENIC ACTIVITY OF YEAST SPECIES" The 7th International Conference on Indoor Air Quality and Climate (Abstract). (In press). (1996)

石黑绫子：“室内空气/房屋灰尘中的酵母菌群和酵母菌种的过敏活性”第七届室内空气质量和气候国际会议（摘要）。

K.Ito: "Characterization of IgE binding epitopes on Candida albicans of enolase" J.of Clinical Experimental of Allergy. 25. 529-535 (1995)

K.Ito：“白色念珠菌烯醇酶 IgE 结合表位的表征”J.of Clinical Experimental of Allergy。

K.Ito: "Detection of IgE antibody against candida albicans enolase and its cross-reactivity to Saccharomyces cerevisiae enolase" Clinical Experimental of Allergy. 25(in press). (1995)

K.Ito：“白色念珠菌烯醇化酶 IgE 抗体的检测及其与酿酒酵母烯醇化酶的交叉反应”过敏临床实验。