Inhalant Allergen Control of Patients with Asthma (Emphasis on Airborne Yeasts)

哮喘患者的吸入性过敏原控制（重点是空气中的酵母菌）

• 批准号: 08672672
• 负责人: ISHIGURO Ayako
• 金额: $ 1.41万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672672/
• 关键词: Environmental Control; Home Care; Candida albicans; Asthma; IgE antibody; Airborne yeasts; Cell Wall Mannan; Crossreactivity; エノラーゼ; IgG抗体

1. Inhalant allergen control (emphasis on fungi) of patients with asthma was performed. Case MT (F,19y.) did not need hospitalization after environmental control and has not experienced severe attack. Caes NS(M,10y.) did not need inhaler but his %FEV 1.0 level is still low (52-79%) for two years.1) Particles counts (2.0mu, 5.0mu) and airborne fungi in the indoor air were tend to increase May compare to October.2) From indoor air sample, Candida spp., Cryptococcus spp. and Rhodotorula spp. were dominantly isolated.3) Wiping with a damp cloth and a chemical cloth, and using a vacuum cleaner were effective for elimination of fungi in the indoor by stamp agar plats.4) PFR of Case NS was increased for two yasrs. When his PFR was below the 200L asthma attack was suggested.2. Crossreactivity of IgE level between Candida albicans and airborne yeasts were studied by immunoblot test and inhibition test.1) IgE antibodies to the 2-ME extract and phosphomannoprotein fractions of C.albicans were present in the sera of 98.0% and 96.8% of patients.2) It is suggest that C.albicans mannoproteins are responsible for the crossreactivity among 12 yeasts species at the IgE level.

1.对哮喘患者进行吸入性过敏原控制（重点是真菌）。MT病例（女性，19岁）环境控制后无需住院，未发生严重发作。病例NS（男性，10岁）不需要吸入器，但他的% FEV1.0水平仍然很低（52-79%），持续两年。1）室内空气中的颗粒物计数（2.0 μ，5.0 μ）和空气中的真菌与5月相比有增加的趋势。2）从室内空气样品中，念珠菌属，隐球菌属和红酵母属（Rhodotorula spp.）3）用湿布、化学布擦拭和真空吸尘器对室内真菌的清除效果较好; 4）病例NS的PFR持续2年升高。当PFR低于200 L时，哮喘发作停止。本文用免疫印迹法和抑制试验研究了白念珠菌与空气传播的酵母菌之间的IgE水平的交叉反应性。1）98.0%和96.8%的患者血清中存在白念珠菌2-ME提取物和磷酸甘露糖蛋白组分的IgE抗体。2）白念珠菌甘露糖蛋白是12种酵母菌之间IgE水平交叉反应的主要原因。

项目成果

石黒彩子: "アレルギー疾患患児の環境整備" 木村看護教育振興財団看護研究集録. 4. 1-5 (1997)

石黑绫子：《过敏性疾病儿童的环境改善》木村护理教育振兴财团护理研究记录4. 1-5 (1997)。

T.Kanbe: "A crossreactivity at the immunoglobulin E level of the cell wall mannoproteins of Candida albicans with other pathogenic Candida and airborne yeast species" Clinical and Experimental Allergy. 27. 1449-1457 (1997)

T.Kanbe：“白色念珠菌细胞壁甘露糖蛋白与其他致病性念珠菌和空气传播酵母菌种在免疫球蛋白 E 水平上的交叉反应”临床和实验过敏。

Ayako Ishiguro: "Detection of IgG Antibodies Specific for Candida albicans Antigens in Children with Acute Lymphoblastic Leukemia" Jpn.J.Med.Mycol.37(3). 167-173 (1996)

Ayako Ishiguro：“急性淋巴细胞白血病儿童白色念珠菌抗原特异性 IgG 抗体的检测”Jpn.J.Med.Mycol.37(3)。

A.Ishiguro: "Environmental control of patients with asthmaemphasis in the indoor air-" J.of Japanese Society of Pediatric Nursing. 5 : (2). 94-99 (1996)

A.Ishiguro：“哮喘患者的环境控制重点是室内空气——”日本儿科护理学会杂志。

石黒彩子: "喘息児宅の訪問調査と指導-室内環境整備を中心として-" 日本小児看護研究会会誌. 5(2). 49-52 (1996)

石黑绫子：“哮喘儿童家庭访问调查和指导 - 重点改善室内环境”日本儿科护理研究会杂志 5（2）（1996）。