Histology and in situ hybridization histochemistry of the intracellular signal transduction system in neurological mutant animals.

神经突变动物细胞内信号转导系统的组织学和原位杂交组织化学。

• 批准号: 06680746
• 负责人: ABE Hiroshi
• 金额: $ 1.47万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680746/
• 关键词: Mutant mouse; Protein phosphatases; Brain; Morphological abnormalities; In situ hybridization; Nissl stain; Neuron; 蛋白質ホスファターゼ; リーラー・マウス; スタゲラ-・マウス; Swaying・マウス; スンクス; 小脳皮質; 組織構築

Neurological mutant mice were investigated to observe morphological abnormalities, such as, degeneration of neurons, disruptions of laminations, abnormal neuronal processes, and abnormal synapse or neuronal circuit formation. We also investigated the localization of neuronal protein and genes, to understand the functional significance of these substances in neurons.Neocortex of dreher mutant mice and hippocampus of weaver mice were observed by histological methods. Various abnormalities were found, such as, aggregation of ectopic Pukinje cells. Morphological abnormalities were not confined to cerebellum, but they were found in various locations in central nervous systems. These reports will be necessary for future study by immunohistopchemistry and in situ hybridization histochemistry.We used in situ hybridization histochemistry to search for the expression of mRNAs of protein phosphatases type 2Abeta, 2B,2Cbeta, in Purkinje cells of normal mice, and staggerer (sg/sg) and reeler (rl/rl) mutant mice, two strains with known Purkinje cell disorders. The expression of the mRNAs was comparable in the normal and reeler Purkinje cells, but considerably reduced in the staggerer Purkinje cells. We interpret this finding as indicating : (a) the staggerer mutant gene may directly affect the phosphatase component of the protein phosphorylation-dephosphorylation cycle in the sg/sg Punkinje cells ; or (b) the reduced mRNA expression may be a secondary phenomenon, resulting from abnormal Purkinje cell function due to the lack of synaptic input.

研究神经突变小鼠以观察形态异常，例如神经元变性、层压破坏、神经元过程异常以及突触或神经元回路形成异常。我们还研究了神经元蛋白和基因的定位，以了解这些物质在神经元中的功能意义。通过组织学方法观察dreher突变小鼠的新皮质和weaver小鼠的海马。发现了各种异常，例如异位普肯野细胞聚集。形态异常不仅限于小脑，还存在于中枢神经系统的多个部位。这些报告对于免疫组织化学和原位杂交组织化学的未来研究是必要的。我们使用原位杂交组织化学来寻找正常小鼠浦肯野细胞以及staggerer (sg/sg)和reeler (rl/rl)突变小鼠的2Abeta、2B、2Cbeta型蛋白磷酸酶mRNA的表达，这两种品系具有已知的 浦肯野细胞疾病。 mRNA 的表达在正常浦肯野细胞和卷曲浦肯野细胞中相当，但在交错浦肯野细胞中显着降低。我们将这一发现解释为：(a) 交错突变基因可能直接影响 sg/sg Punkinje 细胞中蛋白质磷酸化-去磷酸化循环的磷酸酶成分； (b) mRNA 表达减少可能是继发现象，是由于缺乏突触输入而导致浦肯野细胞功能异常所致。

项目成果

郭 煌 他: "スンクス中枢神経系の層構築について." 解剖学雑誌. 69. 546- (1994)

郭 H. 等人：“论 Suncus 中枢神经系统的分层”，《解剖学杂志》69. 546-（1994）。

M.Sekiguchi: "The abnormal distribution of mossy fiber bundles and morphological abnormalities in hippocampal formation of dreher^J (dr^J/dr^J) mouse." Developmental Brain Research. (in press).

M.Sekiguchi：“dreher^J (dr^J/dr^J) 小鼠海马结构中苔藓纤维束的异常分布和形态异常。”

H. Guo: "Protein phosphatase mRNA exression in Purkinje cells of staggerer and reeler mutant mice" Molecular Brain Research. 33. 121-126 (1995)

H.Guo：“staggerer 和 reeler 突变小鼠浦肯野细胞中的蛋白磷酸酶 mRNA 表达”分子脑研究。

Masaki Sekiguchi et al.: "Abnormal morphogenesis of midbrain and cerekellum in swaying,a mutation of segment polarity gene,WNT-1" Neuroscience Research. S.19. S125- (1994)

Masaki Sekiguchi等人：“中脑和小脑摇摆时的形态发生异常，节段极性基因突变，WNT-1”神经科学研究。

M. Sekiguchi: "The abnormal distribution of mossy fiber bundles and morphological abnormalities in hippocampal formation of dreher^J (dr^J/dr^J) mouse" Developmental Brain Research. (in press).

M. Sekiguchi：“dreher^J (dr^J/dr^J)小鼠海马结构中苔藓纤维束的异常分布和形态异常”发育脑研究。